Mouse CD3 Monoclonal Antibody | anti-CD3 antibody

MOUSE ANTI HUMAN CD3:Low Endotoxin

Cat. Num. AAA211260

• Gene Names: CD3G; T3G; IMD17; CD3-GAMMA
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Synonyms: CD3; Monoclonal Antibody; MOUSE ANTI HUMAN CD3:Low Endotoxin; anti-CD3 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: UCHT1
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1mg/ml (varies by lot)
• Sequence Length: 182

• Applicable Applications for anti-CD3 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 100ul of whole blood. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC (MBS211022))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750 (MBS219122P750))

Testing Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. High power)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3 (MBS219122))

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405 (MBS219122A405))

Testing Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. High power)

Testing Data

(ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 (MBS215051) as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 (MBS213023) as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)

Testing Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. Low power)

Testing Data

(Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE (MBS213832))

Testing Data

(Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody (MBS235411) as a detection reagent. Medium power)

NCBI and Uniprot Product Information

• NCBI GI #: 4557429
• NCBI GeneID: 917
• NCBI Accession #: NP_000064.1
• NCBI GenBank Nucleotide #: NM_000073.2
• Molecular Weight: 20,469 Da
• NCBI Official Full Name: T-cell surface glycoprotein CD3 gamma chain
• NCBI Official Synonym Full Names: CD3g molecule, gamma (CD3-TCR complex)
• NCBI Official Symbol: CD3G
• NCBI Official Synonym Symbols: T3G; IMD17; CD3-GAMMA
• NCBI Protein Information: T-cell surface glycoprotein CD3 gamma chain; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); T-cell antigen receptor complex, gamma subunit of T3; T-cell receptor T3 gamma chain
• UniProt Protein Name: T-cell surface glycoprotein CD3 gamma chain
• Protein Family: CD302 antigen
• UniProt Gene Name: CD3G
• UniProt Synonym Gene Names: T3G
• UniProt Entry Name: CD3G_HUMAN

NCBI Description

The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency. [provided by RefSeq, Jul 2008]

Uniprot Description

CD3G: The CD3 complex mediates signal transduction.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; alpha-beta T cell receptor complex

Molecular Function: transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; T cell receptor binding

Biological Process: protein transport; regulation of immune response; cell surface receptor linked signal transduction; T cell activation; T cell costimulation; establishment and/or maintenance of cell polarity; innate immune response; protein complex assembly; T cell receptor signaling pathway

Disease: Immunodeficiency 17