Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Mouse Arginylation Monoclonal Antibody
N-terminal Arginylation Antibody, Clone 4A9: PerCP
Reactivity
Species Independent
Applications
Western Blot, Flow Cytometry, Functional Assay
Purity
Protein G Purified
Synonyms
Arginylation; Monoclonal Antibody; N-terminal Arginylation Antibody; Clone 4A9: PerCP; Mouse Anti-N-terminal Arginylation Monoclonal IgG1; N-terminal Arginylation Antibody: PerCP; N-terminal Arginine Antibody; N-terminal Arginylated Antibody; N terminal Arginine Antibody; N terminal Arginylation Antibody; N terminal Arginylated Antibody; Amino-terminal Arginine Antibody; Amino-terminal Arginylation Antibody; Amino-terminal Arginylated Antibody; Amino terminal Arginine Antibody; Amino terminal Arginylation Antibody; Amino terminal Arginylated Antibody; anti-Arginylation antibody
Host
Mouse
Reactivity
Species Independent
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4A9
Specificity
Specific for N-terminal arginine next to glutamic acid. Does not detect N-terminal arginine next to aspartic acid or internal arginine residues.
Purity/Purification
Protein G Purified
Concentration
1 mg/ml (varies by lot)
Applicable Applications for anti-Arginylation antibody
Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000
FC/FACS: 1:50
FC/FACS: 1:50
Target
N-terminal Arginylation
Immunogen
Synthetic N-terminal arginylated peptide conjugated to KLH
Cellular Localization
Endoplasmic Reticulum
Conjugate
PerCP
Storage Buffer
PBS pH 7.4, 50% glycerol, 0.09% Sodium Azide
Preparation and Storage
Store at -20 degree C.
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42 degree C for 2 hours.)
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Heat stressed cells were subject to heat shock at 42 degree C for 2 hours.)
Related Product Information for anti-Arginylation antibody
Protein arginylation is the post-translational addition of arginine to proteins by arginyltransferase ATE1. Arginylation of proteins has been found to play an essential role in physiological pathways during embryogenesis and adulthood (1). Arginylation has also been shown to regulate cell stress responses, including ER stress, cytosolic misfolded proteins, and heat stress (2).
Product Categories/Family for anti-Arginylation antibody
References
1. Saha S. and Kashina A. (2011) Dev Biol. 385(1): 1-8. 2. Deka K., et al. (2016) Cell Death Discov. 2: 16074.
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Product Notes
The Arginylation (Catalog #AAA808391) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The N-terminal Arginylation Antibody, Clone 4A9: PerCP reacts with Species Independent and may cross-react with other species as described in the data sheet. AAA Biotech's Arginylation can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Flow Cytometry (FC/FACS). WB: 1:1000 FC/FACS: 1:50. Researchers should empirically determine the suitability of the Arginylation for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arginylation, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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