Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22347c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22347c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit GPX7 Polyclonal Antibody | anti-GPX7 antibody
GPX7 Antibody (Center)
Gene Names
GPX7; GPX6; CL683; GPx-7; NPGPx; GSHPx-7
Reactivity
HumanPredicted: Bovine
Applications
Flow Cytometry, Functional Assay, Western Blot, ELISA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Synonyms
GPX7; Polyclonal Antibody; GPX7 Antibody (Center); Purified Rabbit Polyclonal Antibody (Pab); Glutathione peroxidase 7; GPx-7; GSHPx-7; 1.11.1.9; CL683; GPX6; anti-GPX7 antibody
Host
Rabbit
Reactivity
Human
Predicted: Bovine
Predicted: Bovine
Clonality
Polyclonal
Isotype
Ig
Specificity
This GPX7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 73-107 amino acids from the Central region of human GPX7.
Purity/Purification
Purified through a protein A column, followed by peptide affinity purification.
Form/Format
Supplied in PBS with 0.09% (W/V) sodium azide.
Sequence Length
187
Applicable Applications for anti-GPX7 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)
Application Notes
FC/FACS: 1:25
WB: 1:2000
WB: 1:2000
Antigen Type
Synthesized Peptide
Antigen Source
Human
Tissue Location
Expressed in esophageal epithelial cells; expression is up-regulated after exposure to acidic bile acids
Cellular Location
Secreted.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22347c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22347c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing U-2 OS cells stained with AP22347c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22347c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot (WB)
(All lanes : Anti-GPX7 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: THP-1 whole cell lysateLane 3: RPMI 8226 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-GPX7 Antibody (Center) at 1:2000 dilutionLane 1: Hela whole cell lysateLane 2: THP-1 whole cell lysateLane 3: RPMI 8226 whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-GPX7 antibody
It protects esophageal epithelia from hydrogen peroxide- induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks.
Product Categories/Family for anti-GPX7 antibody
References
Gu S.,et al. Submitted (NOV-2000) to the EMBL/GenBank/DDBJ databases. Clark H.F.,et al.Genome Res. 13:2265-2270(2003). Ota T.,et al.Nat. Genet. 36:40-45(2004). Gregory S.G.,et al.Nature 441:315-321(2006). Barrow I.K.-P.,et al. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20996
NCBI Official Full Name
glutathione peroxidase 7
NCBI Official Synonym Full Names
glutathione peroxidase 7
NCBI Official Symbol
GPX7
NCBI Official Synonym Symbols
GPX6; CL683; GPx-7; NPGPx; GSHPx-7
NCBI Protein Information
glutathione peroxidase 7
UniProt Protein Name
Glutathione peroxidase 7
Protein Family
UniProt Gene Name
GPX7
UniProt Synonym Gene Names
GPX6; GPx-7; GSHPx-7
Uniprot Description
It protects esophageal epithelia from hydrogen peroxide-induced oxidative stress. It suppresses acidic bile acid-induced reactive oxigen species (ROS) and protects against oxidative DNA damage and double-strand breaks.
Research Articles on GPX7
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Product Notes
The GPX7 gpx7 (Catalog #AAA9231295) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GPX7 Antibody (Center) reacts with Human Predicted: Bovine and may cross-react with other species as described in the data sheet. AAA Biotech's GPX7 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). FC/FACS: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the GPX7 gpx7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPX7, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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