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Type
Reactivity
Gene Name
Isotype
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Application
Clone
10000 results for "Goat IgG Fc " - showing 6050-6100
Western Blot (WB)
(Western blot analysis of CEBP alpha expression in U937 cell lysate (MBS179300).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBPA )
CEBP alpha, Monoclonal Antibody (Cat# AAA179300)
Full Name
Anti-CEBP alpha Rabbit Monoclonal Antibody
Gene Names
CEBPA; CEBP; C/EBP-alpha
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Catenin alpha 1 expression in HeLa cell lysate (MBS179706).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTNNA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTNNA1 )
Catenin alpha 1, Monoclonal Antibody (Cat# AAA179706)
Full Name
Anti-Catenin alpha 1 Rabbit Monoclonal Antibody
Gene Names
CTNNA1; MDPT2; CAP102
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-NF-Kappa B p65 (S529) expression in HeLa cell lysates treated with Calyculin A and TNF-alpha (MBS1750229).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RELA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RELA )
NF-Kappa B p65, Monoclonal Antibody (Cat# AAA1750229)
Full Name
Anti-Phospho-NF-Kappa B p65 (S529) Rabbit Monoclonal Antibody
Gene Names
RELA; p65; NFKB3
Reactivity
Human
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human prostate, using Desmin Antibody(MBS179775)DES was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-DES Antibody (MBS179775)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Desmin, Monoclonal Antibody (Cat# AAA179775)
Full Name
Anti-Desmin Rabbit Monoclonal Antibody
Gene Names
DES; CSM1; CSM2; CDCD3; CMD1F; LGMD1D; LGMD1E; LGMD2R
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of EGFR using anti-EGFR antibody (M00023-2).EGFR was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-EGFR Antibody (M00023-2) overnight at 4° C. Biotinylated goat anti Rabbit IgG IgG antibody was used as secondary antibody and incubated for 30 minutes at 37° C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. Figure 1. Western blot analysis of EGFR using anti-EGFR antibody (M00023-2).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EGFR. Previous Next )
EGFR (ErbB 1), Monoclonal Antibody (Cat# AAA1751899)
Full Name
Anti-EGFR (ErbB 1) Monoclonal Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using CD11b Antibody(MBS179139)ITGAM was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ITGAM Antibody (MBS179139)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CD11b, Monoclonal Antibody (Cat# AAA179139)
Full Name
Anti-CD11b Rabbit Monoclonal Antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil, using Ki67 Antibody (MBS179225)MKI67 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MKI67 Antibody (MBS179225)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Ki67, Monoclonal Antibody (Cat# AAA179225)
Full Name
Anti-Ki67 Rabbit Monoclonal Antibody
Gene Names
MKI67; KIA; MIB-; MIB-1; PPP1R105
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Hexokinase 1 Antibody(MBS179676)HK1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HK1 Antibody (MBS179676)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Hexokinase 1, Monoclonal Antibody (Cat# AAA179676)
Full Name
Anti-Hexokinase 1 Rabbit Monoclonal Antibody
Gene Names
HK1; HK; HKD; HKI; HXK1; RP79; HMSNR; HK1-ta; HK1-tb; HK1-tc; hexokinase
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD31 expression in THP1 cell lysate (MBS179680).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PECAM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PECAM1 )
CD31, Monoclonal Antibody (Cat# AAA179680)
Full Name
Anti-CD31 Rabbit Monoclonal Antibody
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CD11b using anti-CD11b antibody (MBS1750334).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: mouse spleen tissue lysates,Lane 4: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD11b antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD11b at approximately 170KD. The expected band size for CD11b is at 127KD. )
CD11b, Polyclonal Antibody (Cat# AAA1750334)
Full Name
Anti-CD11b Picoband antibody
Gene Names
ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Stathmin 1 expression in PC-12 cell lysate (MBS179585).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STMN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STMN1 )
Stathmin 1, Monoclonal Antibody (Cat# AAA179585)
Full Name
Anti-Stathmin 1 Rabbit Monoclonal Antibody
Gene Names
STMN1; Lag; SMN; OP18; PP17; PP19; PR22; LAP18; C1orf215
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Figure 2. IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (MBS1753594).ApoER2/LRP8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (MBS1753594) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # MBS176451) with DAB as the chromogen. )
ApoER2/LRP8, Polyclonal Antibody (Cat# AAA1753594)
Full Name
Anti-ApoER2/LRP8 Antibody
Gene Names
LRP8; MCI1; LRP-8; APOER2; HSZ75190
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(SOX1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the SOX1 antibody detected the SOX1 protein (arrow).)
SOX1, Polyclonal Antibody (Cat# AAA6349908)
Full Name
SOX1, NT (SOX1, Transcription factor SOX-1) (MaxLight 405)
Reactivity
Human
Applications
FC/FACS, WB
Purity
Purified by Protein A and peptide affinity chromatography
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
IgG [H&L], Secondary Antibody (Cat# AAA417853)
Full Name
Cy5 Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey]
Reactivity
Goat
Applications
FC/FACS
Pricing
$290/0.25 mg
IgG [H&L], Secondary Antibody (Cat# AAA418004)
Full Name
Texas Red Conjugated Affinity Purified anti-Mouse IgG [H&L] [Goat]
Reactivity
Mouse
Applications
FC/FACS
Pricing
$265/0.25 mg
IgG [H&L], Secondary Antibody (Cat# AAA417974)
Full Name
Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]
Reactivity
Rabbit
Applications
FC/FACS
Pricing
$290/0.25 mg
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of PBMC, staining CD14. The sample was incubated with the primary antibody at 1:100 for 30 min, at 4 deg C. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Gating Strategy: Lymphocyte)
CD14, Monoclonal Antibody (Cat# AAA5310736)
Full Name
CD14 antibody
Applications
EIA, WB
Purity
Purified by Protein A affinity chromatography
Pricing
$420/0.1 mL | $1,880/5x0.1 mL
Western Blot (WB)
(Western blot analysis of VAMP2 expression in mouse brain lysate (MBS179828).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAMP2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VAMP2 )
VAMP2, Monoclonal Antibody (Cat# AAA179828)
Full Name
Anti-VAMP2 Rabbit Monoclonal Antibody
Gene Names
VAMP2; SYB2; VAMP-2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Niemann Pick C1 on SiHa cell lysate using anti-Niemann Pick C1 antibody at 1/500 dilution.)
Niemann Pick C1, Monoclonal Antibody (Cat# AAA9436577)
Full Name
Niemann Pick C1 Antibody
Gene Names
NPC1; NPC
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of beta II Tubulin expression in C6 cell lysate (MBS1750112).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBB4B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TUBB4B )
beta II Tubulin, Monoclonal Antibody (Cat# AAA1750112)
Full Name
Anti-beta II Tubulin Rabbit Monoclonal Antibody
Gene Names
TUBB4B; Beta2; TUBB2; TUBB2C
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PAX7 expression in HeLa lysate (MBS179473).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAX7 )
PAX7, Monoclonal Antibody (Cat# AAA179473)
Full Name
Anti-PAX7 Rabbit Monoclonal Antibody
Gene Names
PAX7; HUP1; RMS2; PAX7B
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Nesprin 1 on different lysates using anti-Nesprin 1 antibody at 1/500 dilution. Positive control: Lane 1: A549 Lane 2: Mouse spleen tissue)
Nesprin 1, Monoclonal Antibody (Cat# AAA9436550)
Full Name
Nesprin 1 Antibody
Gene Names
SYNE1; 8B; CPG2; ARCA1; EDMD4; MYNE1; Nesp1; SCAR8; C6orf98; dJ45H2.2
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Caspase-14 expression in Human skin lysate (MBS1750040).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP14 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CASP14 )
Caspase-14, Monoclonal Antibody (Cat# AAA1750040)
Full Name
Anti-Caspase-14 Rabbit Monoclonal Antibody
Gene Names
CASP14; ARCI12
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Smad5 expression in HEK293 cell lysate (MBS179652).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMAD5 )
Smad5, Monoclonal Antibody (Cat# AAA179652)
Full Name
Anti-Smad5 Rabbit Monoclonal Antibody
Gene Names
SMAD5; DWFC; JV5-1; MADH5
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PKA 2 beta expression in human fetal brain lysate (MBS1750031).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAR2B monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAR2B )
PKA 2 beta, Monoclonal Antibody (Cat# AAA1750031)
Full Name
Anti-PKA 2 beta Rabbit Monoclonal Antibody
Gene Names
PRKAR2B; PRKAR2; RII-BETA
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PIAS1+PIAS2+PIAS3 expression in HepG2 cell lysate (MBS179723).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PIAS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PIAS1 )
PIAS1+PIAS2+PIAS3, Monoclonal Antibody (Cat# AAA179723)
Full Name
Anti-PIAS1+PIAS2+PIAS3 Rabbit Monoclonal Antibody
Gene Names
PIAS1; GBP; ZMIZ3; DDXBP1; GU/RH-II
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human kidney, using Tyrosine Hydroxylase Antibody (MBS179765)TH was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TH Antibody (MBS179765)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Tyrosine Hydroxylase, Monoclonal Antibody (Cat# AAA179765)
Full Name
Anti-Tyrosine Hydroxylase Rabbit Monoclonal Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of TCF7L2 expression in Jurkat cell lysate (MBS179320).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCF7L2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCF7L2 )
TCF7L2, Monoclonal Antibody (Cat# AAA179320)
Full Name
Anti-TCF7L2 Rabbit Monoclonal Antibody
Gene Names
TCF7L2; TCF4; TCF-4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD59 expression in HUVEC cell lysate (MBS179488).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD59 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD59 )
CD59, Monoclonal Antibody (Cat# AAA179488)
Full Name
Anti-CD59 Rabbit Monoclonal Antibody
Gene Names
CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20
Reactivity
Human
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of HDAC8 expression in HeLa cell lysate (MBS179749).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC8 )
HDAC8/Histone Deacetylase 8, Monoclonal Antibody (Cat# AAA179749)
Full Name
Anti-HDAC8/Histone Deacetylase 8 Rabbit Monoclonal Antibody
Gene Names
HDAC8; HD8; WTS; RPD3; CDA07; CDLS5; MRXS6; HDACL1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho- Erk1 (T202/Y204) + Erk2 (T185/Y187) expression in A431 cell lysate treated with EGF (MBS1750209).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK3 )
Erk1, Monoclonal Antibody (Cat# AAA1750209)
Full Name
Anti-Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) Rabbit Monoclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PRP19 expression in (1) HepG2 cell lysate; (2) NIH/3T3 cell lysate (MBS179853).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRPF19 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRPF19 )
PRP19, Monoclonal Antibody (Cat# AAA179853)
Full Name
Anti-PRP19 Rabbit Monoclonal Antibody
Gene Names
PRPF19; PSO4; SNEV; PRP19; UBOX4; hPSO4; NMP200
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22340a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22340a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
MGEA5, Polyclonal Antibody (Cat# AAA9231287)
Full Name
MGEA5 Antibody (N-Term)
Gene Names
MGEA5; OGA; MEA5; NCOAT
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
IHC, FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of PARK7 expression in HeLa cell lysate (MBS179440).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARK7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PARK7 )
PARK7/Dj 1, Monoclonal Antibody (Cat# AAA179440)
Full Name
Anti-PARK7/Dj 1 Rabbit Monoclonal Antibody
Gene Names
PARK7; DJ1; DJ-1; GATD2; HEL-S-67p
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human tonsil, using CD86 Antibody (MBS179198)CD86 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD86 Antibody (MBS179198)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CD86/B7 2, Monoclonal Antibody (Cat# AAA179198)
Full Name
Anti-CD86/B7 2 Rabbit Monoclonal Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p38 Antibody(MBS179165)MAPK14 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MAPK14 Antibody (MBS179165)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
p38, Monoclonal Antibody (Cat# AAA179165)
Full Name
Anti-p38 Rabbit Monoclonal Antibody
Gene Names
MAPK14; RK; p38; CSBP; EXIP; Mxi2; CSBP1; CSBP2; CSPB1; PRKM14; PRKM15; SAPK2A; p38ALPHA
Reactivity
Human, Mouse, Rat
Applications
ChIP, FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of using Integrin beta1 expression in 293T cell lysate (MBS179448).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITGB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ITGB1 )
Integrin beta 1, Monoclonal Antibody (Cat# AAA179448)
Full Name
Anti-Integrin beta 1 Rabbit Monoclonal Antibody
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
(Figure 1:Apoptosis induced in Jurkat T leukemia cells by Killer TRAILTM (MBS566041) is completely blocked by mouse MAb to TRAIL (human) (2E5) in a concentration range of 0.5-1.5µg/ml. Concentration of MAb 2E5 required for inhibition may vary depending of the cell type studied and the concentration of soluble recombinant human TRAIL used. The neutralizing activity of MAb 2E5 has been confirmed with various sources of soluble recombinant human TRAIL including KillerTRAILTM. Method: Jurkat cells (5x104) were treated with KillerTRAILTM (300ng/ml) in the presence or absence of the MAb to TRAIL (human) (2E5) or a control IgG1 mouse monoclonal antibody. After 16 hours apoptosis was evaluated by forward/sideward scatter (FSC/SSC) combined with propidium iodide (PI) staining analyzed by flow cytometry.")
TRAIL, Monoclonal Antibody (Cat# AAA566327)
Full Name
TRAIL (human) monoclonal antibody (2E5)
Gene Names
TNFSF10; TL2; APO2L; CD253; TRAIL; Apo-2L
Reactivity
Human
Does not cross-react with mouse TRAIL
Does not cross-react with mouse TRAIL
Applications
FC/FACS, FUNC
Purity
Protein A-Affinity purified Sterile filtered
Pricing
$535/0.1 mg | $2,350/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Tigit using anti-Tigit antibody (MBS1753480).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysatesLane 2: rat plasma lysatesLane 3: mouse spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Tigit antigen affinity purified polyclonal antibody (Catalog # MBS1753480) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Tigit at approximately 30KD. The expected band size for Tigit is at 30KD. )
Tigit, Polyclonal Antibody (Cat# AAA1753480)
Full Name
Anti-Tigit Antibody
Gene Names
Tigit; Vstm3
Reactivity
Mouse, Rat
Applications
WB, IHC-P, FC, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG, Polyclonal Secondary Antibody (Cat# AAA669073)
Full Name
FITC conjugated Goat anti Hamster IgG (H+L)
Reactivity
Hamster
Applications
FC/FACS, IF
Purity
Purified
Affinity Chromatography
Affinity Chromatography
Pricing
$200/0.1 mL | $845/5x0.1 mL
IgG, Polyclonal Secondary Antibody (Cat# AAA669067)
Full Name
FITC conjugated Goat anti Rabbit IgG (H+L)
Reactivity
Rabbit
Applications
FC/FACS, IF
Purity
Purified
Affinity Chromatography
Affinity Chromatography
Pricing
$200/0.1 mL | $845/5x0.1 mL
Testing Data
IgG, Secondary Antibody (Cat# AAA679297)
Full Name
Goat F (ab')2 Anti-Human IgG, Mouse ads-HRP
Reactivity
Human IgM and IgA; mouse immunoglobulins and pooled sera; may react with IgG from other species
Applications
EIA, FLISA, FC/FACS
Pricing
$250/1 mL | $980/5x1 mL
IgG [H&L], Secondary Antibody (Cat# AAA417972)
Full Name
Phycoerythrin Conjugated Affinity Purified anti-Human IgG [H&L] [Goat]
Reactivity
Human
Applications
FC/FACS
Pricing
$290/0.25 mg
Flow Cytometry (FC/FACS)
(Fig.1: FLOW Cytometry analysis of CD31 in human PBMC using 0.5ug antibody per 10^6 cells. Green represents isotope control. Red represents anti-CD31 antibody (Clone: PECAM-1). Goat anti-mouse IgG conjugated with PE was used as secondary antibody.)
CD31, Monoclonal Antibody (Cat# AAA669024)
Full Name
Monoclonal Antibody to CD31 (Clone: PECAM-1)
Gene Names
PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM
Reactivity
Human
Applications
IHC, FC/FACS
Purity
Purified
Protein G Chromatography
Protein G Chromatography
Pricing
$195/0.1 mg | $140/0.025 mg | $835/5x0.1 mg
Western Blot (WB)
(Western blot analysis of AMPK gamma 1 expression in HEK293 cell lysate (MBS1750016).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAG1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAG1 )
AMPK gamma 1, Monoclonal Antibody (Cat# AAA1750016)
Full Name
Anti-AMPK gamma 1 Rabbit Monoclonal Antibody
Gene Names
PRKAG1; AMPKG
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Stat-1alpha/beta on different cell lysates using anti-Stat-1alpha/beta antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: MCF-7)
Stat-1 alpha/beta, Polyclonal Antibody (Cat# AAA9435427)
Full Name
Stat-1 alpha/beta Antibody
Gene Names
STAT1; CANDF7; ISGF-3; STAT91
Reactivity
Human, Mouse
Applications
WB, ICC, IHC, FC/FACS
Purity
Peptide affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SOX9 expression in SW480 cell lysate (MBS179166).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SOX9 )
SOX9, Monoclonal Antibody (Cat# AAA179166)
Full Name
Anti-SOX9 Rabbit Monoclonal Antibody
Gene Names
SOX9; CMD1; SRA1; CMPD1; SRXX2; SRXY10
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ALOX5 using anti-ALOX5 antibody (M00847).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ALOX5 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ALOX5. )
5 Lipoxygenase, Monoclonal Antibody (Cat# AAA1752025)
Full Name
Anti-5 Lipoxygenase Monoclonal Antibody
Gene Names
ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ADORA1 using anti-ADORA1 antibody (M01101) on A549 cell lysate.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADORA1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADORA1. )
ADORA1, Monoclonal Antibody (Cat# AAA1752055)
Full Name
Anti-ADORA1 Monoclonal Antibody
Gene Names
ADORA1; RDC7
Reactivity
Human
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling Pdx1 with AP22305c at 1/25 dilution, followed by Dylight 488-conjugated goat anti-rabbit IgG (NK179883) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight 554 Phalloidin (PD18466410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).)
BCL2L10, Polyclonal Antibody (Cat# AAA9231241)
Full Name
BCL2L10 Antibody (Center)
Gene Names
BCL2L10; Boo; Diva; BCL-B; bcl2-L-10
Reactivity
Human
Applications
FC/FACS, IF, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
