Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Goat IgG Fc " - showing 6150-6200
Western Blot (WB)
(Figure 1. Western blot analysis of KCNQ2 using anti-KCNQ2 antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SH-SY5Y whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat brain tissue lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNQ2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNQ2 at approximately 97 kDa. The expected band size for KCNQ2 is at 97 kDa.)
KCNQ2, Polyclonal Antibody (Cat# AAA1753421)
Full Name
Anti-KCNQ2 Antibody
Gene Names
KCNQ2; EBN; BFNC; EBN1; ENB1; BFNS1; EIEE7; HNSPC; KV7.2; KCNA11; KVEBN1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
IgG, Polyclonal Secondary Antibody (Cat# AAA669062)
Full Name
FITC conjugated Goat anti Mouse IgG (H+L)
Reactivity
Mouse
Applications
FC/FACS, IF
Purity
Purified
Affinity Chromatography
Affinity Chromatography
Pricing
$200/0.1 mL | $845/5x0.1 mL
IgG [H&L], Secondary Antibody (Cat# AAA417984)
Full Name
Texas Red Conjugated Affinity Purified anti-Cat IgG [H&L] [Goat]
Reactivity
Cat
Applications
FC/FACS
Pricing
$265/0.25 mg
Testing Data
IgG Fab, Secondary Antibody (Cat# AAA679271)
Full Name
Goat Anti-Mouse IgG Fab-AP
Reactivity
Mouse IgG Fc, IgM, and IgA; may react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on mouse IgG Fab covalently linked to agarose
Pricing
$235/1 mL | $905/5x1 mL
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808385)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$575/0.1 mg | $2,355/5x0.1 mg
Western Blot (WB)
(Western blot analysis of AFP expression in HepG2 cell lysate (MBS179349).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AFP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AFP )
AFP/Alpha 1 Fetoprotein, Monoclonal Antibody (Cat# AAA179349)
Full Name
Anti-AFP/Alpha 1 Fetoprotein Rabbit Monoclonal Antibody
Gene Names
AFP; AFPD; FETA; HPAFP
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of TG using anti-TG antibody (M00359-3).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TG antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TG. )
Thyroglobulin, Monoclonal Antibody (Cat# AAA1751948)
Full Name
Anti-Thyroglobulin Monoclonal Antibody
Gene Names
TG; TGN; AITD3
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD10 expression in Jurkat lysate (MBS1750003).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MME monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MME )
CD10, Monoclonal Antibody (Cat# AAA1750003)
Full Name
Anti-CD10 Rabbit Monoclonal Antibody
Gene Names
MME; NEP; SFE; CD10; CALLA; CMT2T; SCA43
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Histone H4 (acetyl K16) antibody. Counter stained with hematoxylin.)
Histone H4, Monoclonal Antibody (Cat# AAA9436854)
Full Name
Histone H4 (Acetyl K16) Antibody
Gene Names
HIST2H4B; H4/o
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Atg4C expression in Jurkat cell lysate (MBS1750116).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATG4C monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATG4C )
Atg4C, Monoclonal Antibody (Cat# AAA1750116)
Full Name
Anti-Atg4C Rabbit Monoclonal Antibody
Gene Names
ATG4C; APG4C; AUTL1; AUTL3; APG4-C
Reactivity
Human
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Bid expression in Jurkat cell lysate (MBS179430).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BID monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BID )
Bid, Monoclonal Antibody (Cat# AAA179430)
Full Name
Anti-Bid Rabbit Monoclonal Antibody
Gene Names
BID; FP497
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Galectin 3 expression in MCF-7 cell lysate (MBS179393).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGALS3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LGALS3 )
Galectin 3, Monoclonal Antibody (Cat# AAA179393)
Full Name
Anti-Galectin 3 Rabbit Monoclonal Antibody
Gene Names
LGALS3; L31; GAL3; MAC2; CBP35; GALBP; GALIG; LGALS2
Reactivity
Human, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of pro Caspase 3 in HeLa cell lysate (MBS179268).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CASP3 )
pro Caspase 3, Monoclonal Antibody (Cat# AAA179268)
Full Name
Anti-pro Caspase 3 Rabbit Monoclonal Antibody
Gene Names
CASP3; CPP32; SCA-1; CPP32B
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using MHC class 1 Antibody(MBS179178)HLA-A was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HLA-A Antibody (MBS179178)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
MHC class 1, Monoclonal Antibody (Cat# AAA179178)
Full Name
Anti-MHC class 1 Rabbit Monoclonal Antibody
Gene Names
HLA-A; HLAA
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of Human CLU in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human CLU monoclonal antibody (1:300) at 37 degree C 1 hour. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.)
Clusterin, Monoclonal Antibody (Cat# AAA8105243)
Full Name
Anti-Clusterin Antibody, Mouse Monoclonal
Gene Names
CLU; CLI; AAG4; APOJ; CLU1; CLU2; KUB1; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2
Reactivity
Human
Applications
WB, EIA, FC/FACS/FCM, ICC, IF
Purity
Protein A
Pricing
$190/0.05 mL | $240/0.1 mL | $280/0.2 mL | $1,110/5x0.2 mL
Western Blot (WB)
(Western blot analysis of VAMP8 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate; (3) PC-12 cell lysate (MBS179830).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VAMP8 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VAMP8 )
VAMP8/Endobrevin, Monoclonal Antibody (Cat# AAA179830)
Full Name
Anti-VAMP8/Endobrevin Rabbit Monoclonal Antibody
Gene Names
VAMP8; EDB; VAMP-8
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Pig SLA Class II D Q followed by Goat anti Mouse IgG (H/L):FITC .)
SLA CLASS II DQ, Monoclonal Antibody (Cat# AAA225941)
Full Name
MOUSE ANTI PIG SLA CLASS II DQ
Reactivity
Pig
Applications
FC/FACS, IHC, IHC
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Western Blot (WB)
(Western Blot (WB) analysis of Bad Rabbit mAb at 1:500 dilutionLane1:MCF7 whole cell lysate)
Bad, Monoclonal Antibody (Cat# AAA3014057)
Full Name
Bad Rabbit Monoclonal Antibody
Gene Names
BAD; BBC2; BCL2L8
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
Protein A affinity purified
Pricing
$355/0.05 mL | $470/0.1 mL | $1,855/5x0.1 mL
Rhodamine, Polyclonal Antibody (Cat# AAA8548443)
Full Name
Rhodamine (TRITC) AffiniPure Goat Rabbit IgG
Reactivity
Rabbit
Applications
ICC, IF, FC/FACS
Purity
Affinity Purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
IgG, Secondary Antibody (Cat# AAA689695)
Full Name
Goat anti-Rat IgG, Fc-specific, secondary antibody, Conjugated to SureLight R-Phycoerythrin
Reactivity
Rat
Applications
WB, EIA
Purity
Affinity purified using solid phase biotin
Pricing
$200/0.1 mg | $745/5x0.1 mg
IgG [H&L], Secondary Antibody (Cat# AAA417855)
Full Name
Cy5 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat]
Reactivity
Hamster
Applications
FC/FACS
Pricing
$290/0.25 mg
IgG [H&L], Secondary Antibody (Cat# AAA417873)
Full Name
Cy3 Conjugated Affinity Purified anti-Rat IgG [H&L] [Goat]
Reactivity
Rat
Applications
FC/FACS
Pricing
$290/0.25 mg
IgG [H&L], Secondary Antibody (Cat# AAA417854)
Full Name
Cy2 Conjugated Affinity Purified anti-Hamster IgG [H&L] [Goat]
Reactivity
Hamster
Applications
FC/FACS
Pricing
$290/0.25 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing huuman peripheral blood lymphocytes stained with CD7 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CD7, Monoclonal Antibody (Cat# AAA9231264)
Full Name
Human CD7 Antibody
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Western Blot (WB) analysis of YAP Rabbit mAb at 1:1000 dilutionLane1:HepG2 whole cell lysate)
YAP, Monoclonal Antibody (Cat# AAA3014137)
Full Name
YAP Rabbit Monoclonal Antibody
Gene Names
YAP1; YAP; YKI; COB1; YAP2; YAP65
Reactivity
Human
Applications
WB, ICC, IF, IHC, FC/FACS
Purity
Protein A affinity purified
Pricing
$355/0.05 mL | $470/0.1 mL | $1,855/5x0.1 mL
Western Blot (WB)
(Western blot analysis of 293 cell line lysates (35ug/lane) using C0107-05D. DH6 (arrow) was detected using C0107-05D.)
Cadherin 6, Polyclonal Antibody (Cat# AAA6466383)
Full Name
Cadherin 6, CT (K-Cadherin, Kidney Cadherin, CDH6, Cadherin-6) (APC)
Gene Names
CDH6; CAD6; KCAD
Reactivity
Human
Applications
WB, FC/FACS, IHC
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of BGN using anti-BGN antibody (M02090).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BGN antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BGN. )
Biglycan, Monoclonal Antibody (Cat# AAA1752170)
Full Name
Anti-Biglycan Monoclonal Antibody
Gene Names
BGN; PGI; MRLS; DSPG1; PG-S1; SEMDX; SLRR1A
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p16INK4A antibody. Counter stained with hematoxylin.)
p16INK4A, Monoclonal Antibody (Cat# AAA9435662)
Full Name
p16INK4A Antibody
Gene Names
CDKN2A; ARF; MLM; P14; P16; P19; CMM2; INK4; MTS1; TP16; CDK4I; CDKN2; INK4A; MTS-1; P14ARF; P19ARF; P16INK4; P16INK4A; P16-INK4A
Reactivity
Human
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of S100 alpha 6 on different lysates using anti-S100 alpha 6 antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: Mouse lung)
S100 alpha 6, Monoclonal Antibody (Cat# AAA9436088)
Full Name
S100 alpha 6 Antibody
Gene Names
S100A6; 2A9; PRA; 5B10; CABP; CACY
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of MUC5B using anti-MUC5B antibody (M00719).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC5B antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MUC5B. )
MUC5B, Monoclonal Antibody (Cat# AAA1752012)
Full Name
Anti-MUC5B Monoclonal Antibody
Gene Names
MUC5B; MG1; MUC5; MUC9; MUC-5B
Reactivity
Human
Applications
FC/FACS, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Bcl-2 expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179054).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BCL2 )
Bcl-2, Monoclonal Antibody (Cat# AAA179054)
Full Name
Anti-Bcl-2 Rabbit Monoclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of HP1 alpha expression in SH-SY5Y cell lysate (MBS179889).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CBX5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CBX5 )
HP1 alpha, Monoclonal Antibody (Cat# AAA179889)
Full Name
Anti-HP1 alpha Rabbit Monoclonal Antibody
Gene Names
CBX5; HP1; HP1A; HEL25
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of HSPA4 expression in HepG2 cell lysate (MBS179975).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPA4 )
HSPA4, Monoclonal Antibody (Cat# AAA179975)
Full Name
Anti-HSPA4 Rabbit Monoclonal Antibody
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HEL-S-5a; HS24/P52
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of STAT5A/B expression in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) PC12 cell lysate (MBS179548).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT5A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT5A )
STAT5A/B, Monoclonal Antibody (Cat# AAA179548)
Full Name
Anti-STAT5A/B Rabbit Monoclonal Antibody
Gene Names
STAT5A; MGF; STAT5
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SMAD4 expression in (1) SH-SY5Y cell lysate; (2) NIH/3T3 cell lysate (MBS179078).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMAD4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMAD4 )
Smad4, Monoclonal Antibody (Cat# AAA179078)
Full Name
Anti-Smad4 Rabbit Monoclonal Antibody
Gene Names
SMAD4; JIP; DPC4; MADH4; MYHRS
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Hsp20 expression in mouse skeletal muscle lysate (MBS1750103).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPB6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HSPB6 )
Hsp20, Monoclonal Antibody (Cat# AAA1750103)
Full Name
Anti-Hsp20 Rabbit Monoclonal Antibody
Gene Names
HSPB6; HEL55; Hsp20; PPP1R91
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-POLR2A (S5) expression in (1) HeLa cell lysate; (2) HeLa cell lysate treated with Lambda Phosphatase (MBS1750248).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-POLR2A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for POLR2A )
POLR2A, Monoclonal Antibody (Cat# AAA1750248)
Full Name
Anti-Phospho-POLR2A (S5) Rabbit Monoclonal Antibody
Gene Names
POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD3D expression in Jurkat cell lysate (MBS1750014).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD3D monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD3D )
CD3D, Monoclonal Antibody (Cat# AAA1750014)
Full Name
Anti-CD3D Rabbit Monoclonal Antibody
Gene Names
CD3D; T3D; IMD19; CD3-DELTA
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Calreticulin on different lysates using anti-Calreticulin antibody at 1/1, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: HL-60)
Calreticulin, Monoclonal Antibody (Cat# AAA9435660)
Full Name
Calreticulin Antibody
Gene Names
CALR; RO; CRT; SSA; cC1qR
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cytokeratin 18 expression in (1) HT-29 cell lysate;(2) human kidney lysate (MBS179637).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT18 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT18 )
Cytokeratin 18, Monoclonal Antibody (Cat# AAA179637)
Full Name
Anti-Cytokeratin 18 Rabbit Monoclonal Antibody
Gene Names
KRT18; K18; CK-18; CYK18
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PRC1 expression in HeLa cell lysate (MBS179153).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRC1 )
PRC1, Monoclonal Antibody (Cat# AAA179153)
Full Name
Anti-PRC1 Rabbit Monoclonal Antibody
Gene Names
PRC1; ASE1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CHD4 expression in 293T cell lysate (MBS179565).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHD4 )
CHD4, Monoclonal Antibody (Cat# AAA179565)
Full Name
Anti-CHD4 Rabbit Monoclonal Antibody
Gene Names
CHD4; CHD-4; Mi-2b; SIHIWES; Mi2-BETA
Reactivity
Human
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ADAR using anti-ADAR antibody (M00869).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAR antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAR. )
ADAR1, Monoclonal Antibody (Cat# AAA1752028)
Full Name
Anti-ADAR1 Monoclonal Antibody
Gene Names
ADAR; DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP
Reactivity
Human
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast cancer, using Cyclin B1 Antibody(MBS179438)CCNB1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CCNB1 Antibody (MBS179438)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Cyclin B1, Monoclonal Antibody (Cat# AAA179438)
Full Name
Anti-Cyclin B1 Rabbit Monoclonal Antibody
Gene Names
CCNB1; CCNB
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Annexin A2 expression in HeLa cell lysate (MBS179478).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANXA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ANXA2 )
Annexin A2, Monoclonal Antibody (Cat# AAA179478)
Full Name
Anti-Annexin A2 Rabbit Monoclonal Antibody
Gene Names
ANXA2; P36; ANX2; LIP2; LPC2; CAL1H; LPC2D; ANX2L4; PAP-IV; HEL-S-270
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cytokeratin 14 expression in A431 cell lysate (MBS179654).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT14 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT14 )
Cytokeratin 14, Monoclonal Antibody (Cat# AAA179654)
Full Name
Anti-Cytokeratin 14 Rabbit Monoclonal Antibody
Gene Names
KRT14; K14; NFJ; CK14; EBS3; EBS4
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PKM2 expression in HeLa cell lysate (MBS179577).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PKM monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PKM )
PKM2, Monoclonal Antibody (Cat# AAA179577)
Full Name
Anti-PKM2 Rabbit Monoclonal Antibody
Gene Names
PKM; PK3; TCB; OIP3; PKM2; CTHBP; THBP1; HEL-S-30
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast cancer, using BMP4 Antibody(MBS179262)BMP4 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BMP4 Antibody (MBS179262)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
BMP4, Monoclonal Antibody (Cat# AAA179262)
Full Name
Anti-BMP4 Rabbit Monoclonal Antibody
Gene Names
BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of c-Met expression in 293 cell lysate (MBS179672).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MET monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MET )
Met, Monoclonal Antibody (Cat# AAA179672)
Full Name
Anti-Met (c-Met) Rabbit Monoclonal Antibody
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met; DFNB97
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Integrin linked ILK using anti-Integrin linked ILK antibody (MBS1753563).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat heart tissue lysatesLane 2: rat PC-12 whole cell lysatesLane 3: mouse heart tissue lysatesLane 4: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Integrin linked ILK antigen affinity purified polyclonal antibody (Catalog # MBS1753563) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for Integrin linked ILK at approximately 51KD. The expected band size for Integrin linked ILK is at 51KD. )
Integrin linked ILK, Polyclonal Antibody (Cat# AAA1753563)
Full Name
Anti-Integrin linked ILK Antibody
Gene Names
ILK; P59; ILK-1; ILK-2; p59ILK
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, FC/FACS/FCM, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
