Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Goat IgG Fc " - showing 6000-6050
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808380)
Full Name
N-terminal Arginylation Antibody, Clone 4A9: ATTO 594
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$580/0.1 mg | $2,385/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Cdc25C expression in K562 cell lysate (MBS179634).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDC25C monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CDC25C )
Cdc25C, Monoclonal Antibody (Cat# AAA179634)
Full Name
Anti-Cdc25C Rabbit Monoclonal Antibody
Gene Names
CDC25C; CDC25; PPP1R60
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Catenin gamma expression in (1) A431 cell lysate; (2) NIH 3T3 cell lysate; (3) C6 cell lysate (MBS179760).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JUP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for JUP )
Catenin gamma, Monoclonal Antibody (Cat# AAA179760)
Full Name
Anti-Catenin gamma Rabbit Monoclonal Antibody
Gene Names
JUP; DP3; PDGB; PKGB; CTNNG; DPIII
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing HeLa cells stained with AP22318a(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22318a, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CKS2, Polyclonal Antibody (Cat# AAA9231257)
Full Name
CKS2 Antibody (N-Term)
Gene Names
CKS2; CKSHS2
Reactivity
Human
Predicted: Bovine, Mouse
Predicted: Bovine, Mouse
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Caspase-6 expression in MCF-7 cell lysate (MBS179872).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CASP6 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CASP6 )
Caspase-6, Monoclonal Antibody (Cat# AAA179872)
Full Name
Anti-Caspase-6 Rabbit Monoclonal Antibody
Gene Names
CASP6; MCH2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Calreticulin expression in (1)HeLa cell lysate; (2)HepG2 cell lysate (MBS179484).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CALR )
Calreticulin, Monoclonal Antibody (Cat# AAA179484)
Full Name
Anti-Calreticulin Rabbit Monoclonal Antibody
Gene Names
CALR; RO; CRT; SSA; cC1qR; HEL-S-99n
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MMP12 expression in WI-38 cell lysate (MBS179681).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MMP12 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MMP12 )
MMP12, Monoclonal Antibody (Cat# AAA179681)
Full Name
Anti-MMP12 Rabbit Monoclonal Antibody
Gene Names
MMP12; ME; HME; MME; MMP-12
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Nanog expression in NCCIT cell lysate (MBS179148).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NANOG monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NANOG )
Nanog, Monoclonal Antibody (Cat# AAA179148)
Full Name
Anti-Nanog Rabbit Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of NSE expression in HeLa cell lysate (MBS179914).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ENO2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ENO2 )
NSE, Monoclonal Antibody (Cat# AAA179914)
Full Name
Anti-NSE Rabbit Monoclonal Antibody
Gene Names
ENO2; NSE; HEL-S-279
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human brain carcinoma, using ATP citrate lyase Antibody (MBS179835)ACLY was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ACLY Antibody (MBS179835)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
ATP citrate lyase, Monoclonal Antibody (Cat# AAA179835)
Full Name
Anti-ATP citrate lyase Rabbit Monoclonal Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of RUNX1/RUNX2/RUNX3 expression in MOLT4 cell lysate (MBS179090).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RUNX1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RUNX1 )
RUNX1/RUNX2/RUNX3, Monoclonal Antibody (Cat# AAA179090)
Full Name
Anti-RUNX1/RUNX2/RUNX3 Rabbit Monoclonal Antibody
Gene Names
RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; CBF2alpha; AML1-EVI-1; PEBP2alpha
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PABPN1 expression in HeLa cell lysate (MBS179854).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PABPN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PABPN1 )
PABPN1, Monoclonal Antibody (Cat# AAA179854)
Full Name
Anti-PABPN1 Rabbit Monoclonal Antibody
Gene Names
PABPN1; OPMD; PAB2; PABII; PABP2; PABP-2
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CXCR7 expression in K562 cell lysate (MBS179877).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ACKR3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ACKR3 )
CXCR7, Monoclonal Antibody (Cat# AAA179877)
Full Name
Anti-CXCR7 Rabbit Monoclonal Antibody
Gene Names
ACKR3; RDC1; CXCR7; RDC-1; CMKOR1; CXC-R7; CXCR-7; GPR159
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Rho A + B + C Antibody (MBS179186)RHOA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RHOA Antibody (MBS179186)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Rho A + B + C, Monoclonal Antibody (Cat# AAA179186)
Full Name
Anti-Rho A + B + C Rabbit Monoclonal Antibody
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Survivin in Jurkat cell lysate (MBS179297).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BIRC5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BIRC5 )
Survivin, Monoclonal Antibody (Cat# AAA179297)
Full Name
Anti-Survivin Rabbit Monoclonal Antibody
Gene Names
BIRC5; API4; EPR-1
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western Blot analysis of cell line lysates (35ug/lane) using MBS6480911. HLA-B (arrow) was detected using MBS6480911.)
HLA Class 1 Antigen B, Antibody (Cat# AAA6480911)
Full Name
HLA Class 1 Antigen B, NT (HLA Cass I Histocompatibility Antigen B-27 alpha Chain, HLA B Class I, HLA-B, HLAB, HLA-B27, HLA-B73, AS, MGC111087, MHC Class I Antigen B*27, SPDA1) (Biotin)
Gene Names
HLA-B; AS; HLAB; SPDA1
Applications
EIA, WB, IHC
Purity
Purified by Protein A and peptide affinity chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Immunofluorescence (IF)
(Immunofluorescence staining of A549 cells with MBS7108864 at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C.The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)
FCGR3B, Polyclonal Antibody (Cat# AAA7108864)
Full Name
FCGR3B Antibody
Gene Names
FCGR3B; CD16; FCG3; CD16A; CD16b; FCGR3; FCGR3A; FCR-10; FCRIII; FCRIIIb
Reactivity
Human
Applications
EIA, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PCSK2 Antibody (Center) in 293(lane 1), K562(lane 2) cell line lysates (35ug/lane). PCSK2 (arrow) was detected using the purified Pab.)
Proprotein Convertase 2, Polyclonal Antibody (Cat# AAA6494690)
Full Name
Proprotein Convertase 2 (PC2, KEX2 Like Endoprotease 2, KEX2-like Endoprotease 2, Neuroendocrine Convertase 2, NEC 2, NEC2, Prohormone Convertase 2, Proprotein Convertase Subtilisin/kexin Type 2, PC2, PCSK2, SPC2) (Biotin)
Gene Names
PCSK2; PC2; NEC2; SPC2; NEC 2; NEC-2
Reactivity
Human
Applications
WB, EIA
Purity
Purified by Ammonium Sulfate Precipitation.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western Blot analysis in mouse brain tissue lysates (35ug/lane) usingMBS6007220. This demonstrates that MBS6007220 detected the UTY protein (arrow).)
UTY, Polyclonal Antibody (Cat# AAA6362110)
Full Name
UTY, CT (UTY, Histone demethylase UTY, Ubiquitously-transcribed TPR protein on the Y chromosome, Ubiquitously-transcribed Y chromosome tetratricopeptide repeat protein) (MaxLight 550)
Gene Names
UTY; UTY1; DKFZp686L12190
Reactivity
Human
Applications
WB, IF, FC/FACS
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.1 mL | $3,945/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Argonaute 2 on different lysates using anti-Argonaute 2 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)
Argonaute 2, Monoclonal Antibody (Cat# AAA9436098)
Full Name
Argonaute 2 Antibody
Gene Names
AGO2; Q10; EIF2C2
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MEF2A+MEF2C expression in Raji cell lysate (MBS179567).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEF2C monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MEF2C )
MEF2A+MEF2C, Monoclonal Antibody (Cat# AAA179567)
Full Name
Anti-MEF2A+MEF2C Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ANGPTL4 Antibody (Center) in mouse liver tissue lysates (35ug/lane). ANGPTL4 (arrow) was detected using the purified Pab.)
ANGPTL4, Polyclonal Antibody (Cat# AAA6273483)
Full Name
ANGPTL4, ID (ANGPTL4, ARP4, HFARP, PGAR, Angiopoietin-related protein 4, Angiopoietin-like protein 4, Hepatic fibrinogen/angiopoietin-related protein) (MaxLight 490)
Gene Names
ANGPTL4; NL2; ARP4; FIAF; HARP; PGAR; HFARP; TGQTL; UNQ171; pp1158; ANGPTL2
Reactivity
Human, Mouse
Applications
FC/FACS, IHC, WB
Purity
Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Pricing
$900/0.1 mL | $4,035/5x0.1 mL
Western Blot (WB)
(Western blot analysis of DKK1 expression in HeLa cell lysate (MBS179397).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DKK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DKK1 )
DKK1, Monoclonal Antibody (Cat# AAA179397)
Full Name
Anti-DKK1 Rabbit Monoclonal Antibody
Gene Names
DKK1; SK; DKK-1
Reactivity
Human, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of GPC3 using anti-GPC3 antibody (M01922-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPC3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GPC3. )
Glypican 3, Monoclonal Antibody (Cat# AAA1752150)
Full Name
Anti-Glypican 3 Monoclonal Antibody
Gene Names
GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2
Reactivity
Human
Applications
FC/FACS, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of DNM2 using anti-DNM2 antibody (M01629).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNM2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DNM2. )
Dynamin 2, Monoclonal Antibody (Cat# AAA1752114)
Full Name
Anti-Dynamin 2 Monoclonal Antibody
Gene Names
DNM2; DYN2; CMT2M; DYNII; LCCS5; CMTDI1; CMTDIB; DI-CMTB
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of STAT1 expression in HeLa cell lysate (MBS179050).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-STAT1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for STAT1 )
STAT1, Monoclonal Antibody (Cat# AAA179050)
Full Name
Anti-STAT1 Rabbit Monoclonal Antibody
Gene Names
STAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91
Reactivity
Human, Mouse
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of PDHB using anti-PDHB antibody (M03998).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDHB antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDHB. )
Pyruvate Dehydrogenase E1 beta subunit, Monoclonal Antibody (Cat# AAA1752308)
Full Name
Anti-Pyruvate Dehydrogenase E1 beta subunit Monoclonal Antibody
Gene Names
PDHB; PDHBD; PHE1B; PDHE1B; PDHE1-B
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, ICC, IP, IF, IHC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CUL3 using anti-CUL3 antibody (M00747).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CUL3 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CUL3. )
Cullin 3, Monoclonal Antibody (Cat# AAA1752017)
Full Name
Anti-Cullin 3 Monoclonal Antibody
Gene Names
CUL3; CUL-3; PHA2E
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ATF4 expression in (1) HeLa cell lysate; (2) 3T3 cell lysate (MBS179291).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATF4 )
ATF4, Monoclonal Antibody (Cat# AAA179291)
Full Name
Anti-ATF4 Rabbit Monoclonal Antibody
Gene Names
ATF4; CREB2; TXREB; CREB-2; TAXREB67
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using CDX2 Antibody(MBS179481)CDX2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CDX2 Antibody (MBS179481)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
CDX2, Monoclonal Antibody (Cat# AAA179481)
Full Name
Anti-CDX2 Rabbit Monoclonal Antibody
Gene Names
CDX2; CDX3; CDX-3; CDX2/AS
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Calpain 1 expression in Human fetal kidney lysate (MBS179774).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAPN1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAPN1 )
Calpain 1, Monoclonal Antibody (Cat# AAA179774)
Full Name
Anti-Calpain 1 Rabbit Monoclonal Antibody
Gene Names
CAPN1; CANP; muCL; CANP1; SPG76; CANPL1; muCANP
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ErbB (HER4) expression in (1) Human brain lysate; (2) MCF-7 cell lysate (MBS179247).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ERBB4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ERBB4 )
ErbB (HER4), Monoclonal Antibody (Cat# AAA179247)
Full Name
Anti-ErbB (HER4) Rabbit Monoclonal Antibody
Gene Names
ERBB4; HER4; ALS19; p180erbB4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of VCP expression in HeLa cell lysate (MBS179388).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for VCP )
VCP, Monoclonal Antibody (Cat# AAA179388)
Full Name
Anti-VCP Rabbit Monoclonal Antibody
Gene Names
VCP; p97; TERA; CDC48
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MEK5 expression in Hela cell lysate (MBS179884).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP2K7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP2K7 )
MEK7, Monoclonal Antibody (Cat# AAA179884)
Full Name
Anti-MEK7 Rabbit Monoclonal Antibody
Gene Names
MAP2K7; MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of JNK3 expression in HeLa lysate (MBS1750010).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAPK10 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAPK10 )
JNK3, Monoclonal Antibody (Cat# AAA1750010)
Full Name
Anti-JNK3 Rabbit Monoclonal Antibody
Gene Names
MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of FGF2 expression in K562 cell lysate (MBS179123).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGF2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FGF2 )
FGF2/Fgf Basic, Monoclonal Antibody (Cat# AAA179123)
Full Name
Anti-FGF2/Fgf Basic Rabbit Monoclonal Antibody
Gene Names
FGF2; BFGF; FGFB; FGF-2; HBGF-2
Reactivity
Human
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22343b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22343b, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
KCNH1, Polyclonal Antibody (Cat# AAA9231291)
Full Name
KCNH1 Antibody (C-Term)
Gene Names
KCNH1; EAG; EAG1; ZLS1; TMBTS; h-eag; hEAG1; Kv10.1
Reactivity
Human
Predicted: Human
Predicted: Human
Applications
FC/FACS, WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of SERCA2 expression in HeLa cell lysate (MBS179578).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP2A2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ATP2A2 )
SERCA2, Monoclonal Antibody (Cat# AAA179578)
Full Name
Anti-SERCA2 Rabbit Monoclonal Antibody
Gene Names
ATP2A2; DD; DAR; ATP2B; SERCA2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using Myosin light chain kinase Antibody(MBS179722)MYLK was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MYLK Antibody (MBS179722)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Myosin light chain kinase, Monoclonal Antibody (Cat# AAA179722)
Full Name
Anti-Myosin light chain kinase Rabbit Monoclonal Antibody
Gene Names
MYLK; KRP; AAT7; MLCK; MLCK1; MYLK1; smMLCK; MLCK108; MLCK210; MSTP083
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of STIL/SIL using anti-STIL/SIL antibody (MBS1753683).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat testis tissue lysatesLane 2: rat C6 whole cell lysatesLane 3: rat PC-12 whole cell lysatesLane 4: mouse NIH/3T3 whole cell lysatesLane 5: human Hela whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-STIL/SIL antigen affinity purified polyclonal antibody (Catalog # MBS1753683) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460 ) with Tanon 5200 system. A specific band was detected for STIL/SIL at approximately 170KD. The expected band size for STIL/SIL is at 170KD. )
STIL/SIL, Polyclonal Antibody (Cat# AAA1753683)
Full Name
Anti-STIL/SIL Antibody
Gene Names
STIL; SIL; MCPH7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, FC, EIA
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PAX7 on Hela cells lysates using anti-PAX7 antibody at 1/1, 000 dilution.)
PAX7, Monoclonal Antibody (Cat# AAA9435940)
Full Name
PAX7 Antibody
Gene Names
PAX7; HUP1; RMS2; PAX7B
Reactivity
Human, Mouse, Rat
Applications
WB, FC
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(Western Blot analysis in HepG2 and mouse C2C12 cell line lysates (35ug/lane) using MBS6348738_FC4. This demonstrates the SMAD3 antibody detected the SMAD3 protein (arrow).)
SMAD3, Polyclonal Antibody (Cat# AAA6348738)
Full Name
SMAD3 (S208) (SMAD3, MADH3, Mothers against decapentaplegic homolog 3, JV15-2, SMAD family member 3) (APC)
Gene Names
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$900/0.2 mL | $4,035/5x0.2 mL
IgG, Polyclonal Secondary Antibody (Cat# AAA669072)
Full Name
APC conjugated Goat anti Hamster IgG (H+L)
Reactivity
Hamster
Applications
FC/FACS, IF
Purity
Purified
Affinity Chromatography
Affinity Chromatography
Pricing
$200/0.1 mL | $845/5x0.1 mL
IgG [H&L], Secondary Antibody (Cat# AAA417990)
Full Name
Texas Red Conjugated Affinity Purified anti-Donkey IgG [H&L] [Goat]
Reactivity
Donkey
Applications
FC/FACS
Pricing
$265/0.25 mg
Immunoprecipitation (IP)
(BAK was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25232 of 10 ug. Western blot was performed from the immunoprecipitate using M25232 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction.Predicted band size : 24 KDaObserved band size : 24 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
BAK, Monoclonal Antibody (Cat# AAA435121)
Full Name
BAK Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ROR2 in 293 cell lysates (2 ug/lane), either nontransfected (Lane 1) or transiently transfected with the ROR2 gene (Lane 2) (Origene Technologies).)
ROR2, Polyclonal Antibody (Cat# AAA6498152)
Full Name
ROR2, NT (Tyrosine-protein Kinase Transmembrane Receptor ROR2, Neurotrophic Tyrosine Kinase, Receptor-related 2, NTRKR2) (Azide free) (HRP)
Gene Names
ROR2; BDB; BDB1; NTRKR2
Reactivity
Human
Applications
EIA, WB, IHC
Purity
Purified by Protein G Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of N-terminal Arginine-BSA showing detection of 67 kDa N-terminal Arginylation protein using Mouse Anti-N-terminal Arginylation Monoclonal Antibody, Clone 4A9 . Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA. Lane 3: RDHKH-BSA. Lane 4: REHKH-BSA. Lane 5: HKH-BSA. Lane 6: HKERD-BSA. Lane 7: HKRRE-BSA. Load: 0.5 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-N-terminal Arginylation Monoclonal Antibody at 1:1000 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 67 kDa. Other Band(s): 250kDa, 150kDa, 75kDa REHKH-BSA.)
Arginylation, Monoclonal Antibody (Cat# AAA808376)
Full Name
N-terminal Arginylation Antibody, Clone 4A9
Reactivity
Species Independent
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$535/0.1 mg | $2,160/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Glycogen synthase expression in HeLa cell lysate (MBS179969).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GYS1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GYS1 )
Glycogen synthase, Monoclonal Antibody (Cat# AAA179969)
Full Name
Anti-Glycogen synthase Rabbit Monoclonal Antibody
Gene Names
GYS1; GSY; GYS
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PPP1R1A expression in Rat brain lysate (MBS1750121).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP1R1A monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPP1R1A )
PPP1R1A, Monoclonal Antibody (Cat# AAA1750121)
Full Name
Anti-PPP1R1A Rabbit Monoclonal Antibody
Gene Names
PPP1R1A; I1; IPP1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Ras expression in (1) C6 cell lysate; (2) Jurkat cell lysate (MBS179103).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRAS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NRAS )
Ras, Monoclonal Antibody (Cat# AAA179103)
Full Name
Anti-Ras Rabbit Monoclonal Antibody
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
