Flow Cytometry (FC/FACS)
(Flow cytometry analysis of PBMC, staining CD14. The sample was incubated with the primary antibody at 1:100 for 30 min, at 4 deg C. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Gating Strategy: Lymphocyte)
Mouse CD14 Monoclonal Antibody | anti-CD14 antibody
CD14 antibody
Applications
ELISA, Western Blot
Purity
Purified by Protein A affinity chromatography
Synonyms
CD14; Monoclonal Antibody; CD14 antibody; Mouse Monoclonal CD14 antibody; Monoclonal CD14 antibody; Anti-CD14 antibody; monocyte differentiation antigen CD14 antibody; Myeloid cell-specific leucine-rich glycoprotein antibody; anti-CD14 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
AT87H7
Purity/Purification
Purified by Protein A affinity chromatography
Form/Format
Liquid form in Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide and 10% Glycerol
Concentration
1 mg/ml (varies by lot)
Sequence Length
373
Applicable Applications for anti-CD14 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
WB: 1:3000
ICC: 1:200
IF: 1:200
ICC: 1:200
IF: 1:200
Immunogen
CD14 antibody was raised in Mouse using recombinant human CD14 (20-349aa) purified from E Coli as the immunogen
Preparation and Storage
Store at 4 degree C for short term storage. For long term storage, aliquot and store at-20 degree C.
Avoid repeat freeze/thaw cycles.
Avoid repeat freeze/thaw cycles.
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of PBMC, staining CD14. The sample was incubated with the primary antibody at 1:100 for 30 min, at 4 deg C. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Gating Strategy: Lymphocyte)
Flow Cytometry (FC/FACS)
(Flow cytometry analysis of PBMC, staining CD14. The sample was incubated with the primary antibody at 1:100 for 30 min, at 4 deg C. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Gating Strategy: Lymphocyte)
Related Product Information for anti-CD14 antibody
The protein encoded by this gene is a component of the innate immune system. CD14 exists in two forms, one anchored to the membrane by a glycosylphosphatidylinositol tail (mCD14), the other a soluble form (sCD14). Soluble CD14 either appears after shedding of mCD14 (48kDa) or is directly secreted from intracellular vesicles (56kDa).The x-ray crystal structure of human CD14 reveals a monomeric, bent solenoid structure containing a hydrophobic amino-terminal pocket. CD14 was the first described pattern recognition receptor. CD14 acts as a co-receptor (along with the Toll-like receptor TLR4 and MD-2) for the detection of bacterial lipopolysaccharide (LPS). CD14 can bind LPS only in the presence of lipopolysaccharide-binding protein (LBP). Although LPS is considered its main ligand, CD14 also recognizes other pathogen-associated molecular patterns such as lipoteichoic acid.
Product Categories/Family for anti-CD14 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,667 Da
NCBI Official Full Name
CD14
NCBI Official Synonym Full Names
CD14 molecule
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
CD14
Uniprot Description
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().
Research Articles on CD14
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Product Notes
The CD14 cd14 (Catalog #AAA5310736) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). WB: 1:3000 ICC: 1:200 IF: 1:200. Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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