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Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "Goat IgG Fc " - showing 5850-5900
Western Blot (WB)
(Western blot analysis of p62/SQSTM1 expression in SKBR-3 cell lysate (MBS179250).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SQSTM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SQSTM1 )
p62/SQSTM1, Monoclonal Antibody (Cat# AAA179250)
Full Name
Anti-p62/SQSTM1 Rabbit Monoclonal Antibody
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Aurora A expression in HepG2 cell lysate (MBS179219).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AURKA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AURKA )
Aurora A, Monoclonal Antibody (Cat# AAA179219)
Full Name
Anti-Aurora A Rabbit Monoclonal Antibody
Gene Names
AURKA; AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; PPP1R47
Reactivity
Human, Mouse
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MUC1 expression in T47 D cell lysate (MBS179174).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MUC1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MUC1 )
MUC1, Monoclonal Antibody (Cat# AAA179174)
Full Name
Anti-MUC1 Rabbit Monoclonal Antibody
Gene Names
MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(ICC staining ADFP in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ADFP, Monoclonal Antibody (Cat# AAA9436272)
Full Name
ADFP Antibody
Gene Names
PLIN2; ADFP; ADRP
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, FC
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Western Blot (WB)
(GAS1 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane) usingMBS644674.This demonstrates that MBS644674 detected the GAS1 antibody detected the GAS1 protein (arrow).)
GAS1, Polyclonal Antibody (Cat# AAA6301268)
Full Name
GAS1, NT (GAS1, Growth arrest-specific protein 1) (PE)
Reactivity
Human, Mouse
Applications
EIA, WB, IF, IHC
Purity
Purified by Protein A/G affinity chromatography
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
IgG Fc protein, Immunoglobulin (Cat# AAA5308120)
Full Name
Purified native IgG Fc protein (Goat)
Applications
EIA, WB
Purity
> 95% pure
Pricing
$500/1 mg | $2,240/5x1 mg
IgG Fc, F(ab)'2 Fragment, Secondary Antibody (Cat# AAA689528)
Full Name
Goat anti-Human IgG Fc, F(ab)'2 Fragment (min x w/ Human IgA/IgM, Bovine, Horse, Mouse, Rabbit Serum)
Reactivity
Human.
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum immunoglobulins
light chains on all human immunoglobulins
human IgA or IgM
serum proteins from bovine, mouse, or rabbit
Cross Reactivity: Based on IEP, no reactivity is observed to:
non-immunoglobulin human serum immunoglobulins
light chains on all human immunoglobulins
human IgA or IgM
serum proteins from bovine, mouse, or rabbit
Applications
WB, EIA
Purity
> 90% based on SDS-PAGE Small amounts of intact IgG may be present.
Affinity purified using solid phase Human IgG.
Affinity purified using solid phase Human IgG.
Pricing
$220/0.5 mg | $850/5x0.5 mg
Western Blot (WB)
(Western blot analysis of AMPK beta 1 expression in HeLa cell lysate (MBS179983).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRKAB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRKAB1 )
AMPK beta 1, Monoclonal Antibody (Cat# AAA179983)
Full Name
Anti-AMPK beta 1 Rabbit Monoclonal Antibody
Gene Names
PRKAB1; AMPK; HAMPKb
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of S100 expression in (1) Human skeletal muscle lysate; (2) RAW 2647 cell lysate (MBS179861).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for S100A1 )
S100, Monoclonal Antibody (Cat# AAA179861)
Full Name
Anti-S100 Rabbit Monoclonal Antibody
Gene Names
S100A1; S100; S100A; S100-alpha
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PUMA expression in K562 cell lysate (MBS1750030).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BBC3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BBC3 )
PUMA, Monoclonal Antibody (Cat# AAA1750030)
Full Name
Anti-PUMA Rabbit Monoclonal Antibody
Gene Names
BBC3; JFY1; PUMA; JFY-1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of NCAM expression in SH-SY5Y cell lysate (MBS179171).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NCAM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NCAM1 )
NCAM, Monoclonal Antibody (Cat# AAA179171)
Full Name
Anti-NCAM Rabbit Monoclonal Antibody
Gene Names
NCAM1; CD56; NCAM; MSK39
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rho A expression in (1) HeLa cell lysate; (2) NIH/3T3 cell lysate (MBS179185).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RHOA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RHOA )
Rho A, Monoclonal Antibody (Cat# AAA179185)
Full Name
Anti-Rho A Rabbit Monoclonal Antibody
Gene Names
RHOA; ARHA; ARH12; RHO12; RHOH12
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of TCR alpha using anti-TCR alpha antibody.Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours.Lane 1: recombinant human TCR alpha protein 1ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TCR alpha antigen affinity purified polyclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TCR alpha at approximately 16KD. The expected band size for TCR alpha is at 16KD.)
TCR alpha/TRAC, Polyclonal Antibody (Cat# AAA1751487)
Full Name
Anti-TCR alpha/TRAC Antibody
Gene Names
TRAC; TRA; IMD7; TCRA; TRCA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, ICC, FC/FACS, EIA
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of ARRB1 expression in 293T cell lysate (MBS179807).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARRB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARRB1 )
ARRB1/Beta Arrestin 1, Monoclonal Antibody (Cat# AAA179807)
Full Name
Anti-ARRB1/Beta Arrestin 1 Rabbit Monoclonal Antibody
Gene Names
ARRB1; ARB1; ARR1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human thyroid, using TTF1 Antibody(MBS179627)NKX2-1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-NKX2-1 Antibody (MBS179627)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
TTF1, Monoclonal Antibody (Cat# AAA179627)
Full Name
Anti-TTF1 Rabbit Monoclonal Antibody
Gene Names
NKX2-1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; NMTC1; T/EBP; TITF1; TTF-1; NKX2.1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using PCNA Antibody(MBS179129)PCNA was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PCNA Antibody (MBS179129)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
PCNA, Monoclonal Antibody (Cat# AAA179129)
Full Name
Anti-PCNA Rabbit Monoclonal Antibody
Gene Names
PCNA; ATLD2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of p63 expression in A431 cell lysate (MBS179157).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP63 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP63 )
p63, Monoclonal Antibody (Cat# AAA179157)
Full Name
Anti-p63 Rabbit Monoclonal Antibody
Gene Names
TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Bax in (1) HeLa cell lysate;(2) RAW2647 Whole Cell Lysate (MBS179170).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAX monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAX )
Bax, Monoclonal Antibody (Cat# AAA179170)
Full Name
Anti-Bax Rabbit Monoclonal Antibody
Gene Names
BAX; BCL2L4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Laminin beta 1 expression in A431 cell lysate (MBS179990).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LAMB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LAMB1 )
Laminin beta 1, Monoclonal Antibody (Cat# AAA179990)
Full Name
Anti-Laminin beta 1 Rabbit Monoclonal Antibody
Gene Names
LAMB1; CLM; LIS5
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ICAM1 expression in Raji cell lysate (MBS179159).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICAM1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ICAM1 )
ICAM1/Cd54, Monoclonal Antibody (Cat# AAA179159)
Full Name
Anti-ICAM1/Cd54 Rabbit Monoclonal Antibody
Gene Names
ICAM1; BB2; CD54; P3.58
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of HDAC7 expression in HeLa cell lysate (MBS179763).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC7 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HDAC7 )
HDAC7/Histone Deacetylase 7, Monoclonal Antibody (Cat# AAA179763)
Full Name
Anti-HDAC7/Histone Deacetylase 7 Rabbit Monoclonal Antibody
Gene Names
HDAC7; HD7; HD7A; HDAC7A
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of ASK1 expression in HeLa cell lysates (MBS179491).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MAP3K5 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MAP3K5 )
ASK1, Monoclonal Antibody (Cat# AAA179491)
Full Name
Anti-ASK1 Rabbit Monoclonal Antibody
Gene Names
MAP3K5; ASK1; MEKK5; MAPKKK5
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Rad21 expression in HeLa cell lysate (MBS179755).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAD21 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAD21 )
Rad21, Monoclonal Antibody (Cat# AAA179755)
Full Name
Anti-Rad21 Rabbit Monoclonal Antibody
Gene Names
RAD21; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Prion Protein expression in Hunam fetal brain lysate (MBS179855).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRNP monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRNP )
Prion Protein, Monoclonal Antibody (Cat# AAA179855)
Full Name
Anti-Prion Protein Rabbit Monoclonal Antibody
Gene Names
PRNP; CJD; GSS; PrP; ASCR; KURU; PRIP; PrPc; CD230; AltPrP; p27-30; PrP27-30; PrP33-35C
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of TMS1 expression in U937 cell lysate (MBS179287).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PYCARD monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PYCARD )
TMS1, Monoclonal Antibody (Cat# AAA179287)
Full Name
Anti-TMS1 Rabbit Monoclonal Antibody
Gene Names
PYCARD; ASC; TMS; TMS1; CARD5; TMS-1
Reactivity
Human
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(IGKV A18 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the IGKVA18 antibody detected the IGKVA18 protein (arrow).)
IGKV A18, Polyclonal Antibody (Cat# AAA6309562)
Full Name
IGKV A18, CT (IGKV A18) (PE)
Reactivity
Human
Applications
WB, FC
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Pricing
$880/0.2 mL | $3,945/5x0.2 mL
Western Blot (WB)
(Western blot analysis of p63 on human kidney lysates using anti-p63 antibody at 1/1, 000 dilution.)
p63, Monoclonal Antibody (Cat# AAA9435750)
Full Name
p63 Antibody
Gene Names
TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)
Reactivity
Human, Mouse, Rat
Applications
WB, ICC, IF, IHC, FC
Purity
ProA affinity purified
Pricing
$325/0.05 mL | $445/0.1 mL | $1,860/5x0.1 mL
Testing Data
IgG Fab, Secondary Antibody (Cat# AAA679270)
Full Name
Goat Anti-Mouse IgG Fab-FITC
Reactivity
Mouse IgG Fc, IgM, and IgA; may react with immunoglobulins from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on mouse IgG Fab covalently linked to agarose
Pricing
$180/1 mg | $665/5x1 mg
Western Blot (WB)
(Western blot analysis of SLC2A2 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). SLC2A2 (arrow) was detected using the purified Pab.)
GLUT2, Polyclonal Antibody (Cat# AAA6478112)
Full Name
GLUT2, NT (Solute Carrier Family 2, Facilitated Glucose Transporter Member 2, Glucose Transporter Type 2, Liver, GLUT-2, SLC2A2) (Biotin)
Gene Names
SLC2A2; GLUT2
Reactivity
Human
Applications
EIA, WB, FC/FACS
Purity
Purified by Protein A Affinity Chromatography.
Pricing
$890/0.2 mL | $3,990/5x0.2 mL
Western Blot (WB)
(The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human VIM antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1 : Jurkat cell lysate Lane 2 : K562 cell lysate Lane 3 : 293T cell lysate Lane 4 : A549 cell lysate Lane 5 : SK-OV-3 cell lysate Lane 6 : HeLa cell lysate)
VIM, Monoclonal Antibody (Cat# AAA200459)
Full Name
VIM antibody
Reactivity
Human
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-A affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Tyrosine Hydroxylase expression in PC12 cell lysate (MBS179764).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TH monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TH )
Tyrosine Hydroxylase, Monoclonal Antibody (Cat# AAA179764)
Full Name
Anti-Tyrosine Hydroxylase Rabbit Monoclonal Antibody
Gene Names
TH; TYH; DYT14; DYT5b
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of PLXNA1 using anti-PLXNA1 antibody (M06377).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLXNA1 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4° C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLXNA1. )
Plexin A1, Monoclonal Antibody (Cat# AAA1752377)
Full Name
Anti-Plexin A1 Monoclonal Antibody
Gene Names
PLXNA1; NOV; NOVP; PLXN1; PLEXIN-A1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, ICC, WB
Purity
Affinity-Chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
CD74, Monoclonal Antibody (Cat# AAA808468)
Full Name
CD74 Antibody, Clone 3D7: ATTO 488
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PAI1 expression in HepG2 cell lysate (MBS179401).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINE1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SERPINE1 )
PAI1, Monoclonal Antibody (Cat# AAA179401)
Full Name
Anti-PAI1 Rabbit Monoclonal Antibody
Gene Names
SERPINE1; PAI; PAI1; PAI-1; PLANH1
Reactivity
Human
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CD81 expression in Ramos cell lysate (MBS179620).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD81 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD81 )
CD81, Monoclonal Antibody (Cat# AAA179620)
Full Name
Anti-CD81 Rabbit Monoclonal Antibody
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of GR expression in A431 cell lysate (MBS179346).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR3C1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NR3C1 )
GR, Monoclonal Antibody (Cat# AAA179346)
Full Name
Anti-GR Rabbit Monoclonal Antibody
Gene Names
NR3C1; GR; GCR; GRL; GCCR; GCRST
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Neutrophil Elastase expression in HL60 cell lysate (MBS179704).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ELANE monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ELANE )
Neutrophil Elastase, Monoclonal Antibody (Cat# AAA179704)
Full Name
Anti-Neutrophil Elastase Rabbit Monoclonal Antibody
Gene Names
ELANE; GE; NE; HLE; HNE; ELA2; SCN1; PMN-E
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of CEBP beta expression in MCF-7 cell lysate (MBS179553).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CEBPB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CEBPB )
CEBP beta, Monoclonal Antibody (Cat# AAA179553)
Full Name
Anti-CEBP beta Rabbit Monoclonal Antibody
Gene Names
CEBPB; TCF5; IL6DBP; NF-IL6; C/EBP-beta
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon, using Musashi 1 Antibody (MBS1750038)MSI1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-MSI1 Antibody (MBS1750038)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Musashi 1, Monoclonal Antibody (Cat# AAA1750038)
Full Name
Anti-Musashi 1 Rabbit Monoclonal Antibody
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of MLH1 in 293T cell lysate (MBS179130).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLH1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLH1 )
MLH1, Monoclonal Antibody (Cat# AAA179130)
Full Name
Anti-MLH1 Rabbit Monoclonal Antibody
Gene Names
MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of SMC3 expression in HeLa cell lysate (MBS179770).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SMC3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SMC3 )
SMC3, Monoclonal Antibody (Cat# AAA179770)
Full Name
Anti-SMC3 Rabbit Monoclonal Antibody
Gene Names
SMC3; BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Cytokeratin 17 expression in HACAT cell lysate (MBS179823).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT17 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT17 )
Cytokeratin 17, Monoclonal Antibody (Cat# AAA179823)
Full Name
Anti-Cytokeratin 17 Rabbit Monoclonal Antibody
Gene Names
KRT17; PC; K17; PC2; 39.1; CK-17; PCHC1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human colon carcinome, using GSK3 beta Antibody(MBS179455)GSK3B was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-GSK3B Antibody (MBS179455)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
GSK3 beta, Monoclonal Antibody (Cat# AAA179455)
Full Name
Anti-GSK3 beta Rabbit Monoclonal Antibody
Reactivity
Human, Mouse
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Lamin A/C expression in HeLa whole cell lysates (MBS179325).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LMNA monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LMNA )
Lamin A/C, Monoclonal Antibody (Cat# AAA179325)
Full Name
Anti-Lamin A/C Rabbit Monoclonal Antibody
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemistry staining of rat liver, with induced hepatocellular damage, using MBS6253681.)
CD68, Monoclonal Antibody (Cat# AAA6253681)
Full Name
CD68 (DKFZp686M18236, GP110, Macrophage Antigen CD68 (Microsialin), Macrosialin, Scavenger Receptor Class D Member 1, SCARD1) (MaxLight 490)
Applications
FC/FACS, IF, IHC, IP, WB
Purity
Purified by protein A affinity chromatography from tissue culture supernatant.
Pricing
$865/0.1 mL | $3,885/5x0.1 mL
Ig Fc, Secondary Antibody (Cat# AAA679299)
Full Name
Goat Anti-Human Ig Fc-BIOT
Reactivity
Human IgG Fab; may react with immunoglobulins from other species and the Fc region of other human immunoglobulins
Applications
EIA
Purity
Affinity chromatography
Pricing
$185/0.5 mg | $680/5x0.5 mg
Testing Data
IgG Fc, Secondary Antibody (Cat# AAA679292)
Full Name
Goat Anti-Human IgG Fc, Multi-Species SP ads-PE
Reactivity
Human IgG Fab, IgM, and IgA; rabbit, mouse, rat, bovine, horse, hamster, goat, sheep, chicken, and guinea pig serum proteins (SP); may react with IgG from other species
Applications
EIA, FLISA
Purity
Affinity chromatography on human IgG Fc covalently linked to agarose
Pricing
$335/0.25 mg | $1,370/5x0.25 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Bovine CD21 and RPE conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Bovine CD21 and Mouse anti Bovine WC4 detected with Goat anti Mouse IgG1:RPE . All experiments performed on bovine red blood cell lysed blood gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
WC4, Monoclonal Antibody (Cat# AAA225844)
Full Name
Mouse Anti Bovine WC4
Reactivity
Bovine
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A.
Pricing
$405/0.1 mg | $1,660/5x0.1 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing huuman peripheral blood monocytes stained with CD14 antibody(green line). The cells were icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:50 dilution) for 60min at 37 degree C. The secondary antibody used was Goat Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40min at 37 degree C. Isotype control antibody (blue line) was mouse IgG2b (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
CD14, Monoclonal Antibody (Cat# AAA9231265)
Full Name
Human CD14 Antibody
Reactivity
Human
Applications
FC/FACS, EIA
Purity
Purified through a protein G column, followed by dialysis against PBS.
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Cytokeratin 16 expression in HaCat cell lysate (MBS179958).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KRT16 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KRT16 )
Cytokeratin 16, Monoclonal Antibody (Cat# AAA179958)
Full Name
Anti-Cytokeratin 16 Rabbit Monoclonal Antibody
Gene Names
KRT16; K16; PC1; CK16; K1CP; NEPPK; FNEPPK; KRT16A
Reactivity
Human
Applications
FC/FACS, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
