IgG [H&L] Secondary Antibody | IgG secondary antibody
Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]
Reactivity
Rabbit
Applications
Flow Cytometry, Functional Assay
Synonyms
IgG [H&L]; Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat]; IgG secondary antibody
Host
Goat
Reactivity
Rabbit
Concentration
1.0 mg/ml (by UV absorbance at 280 nm). OD566nm/OD280nm ratio: 2.0 (varies by lot)
Applicable Applications for IgG secondary antibody
Immunomicroscopy, Flow Cytometry (FC/FACS), Other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity.
Application Notes
The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.
Immunogen
Rabbit IgG whole molecule
Absorption Wavelength
488 nm
Storage Buffer
0.02 M Potassium Phosphate,0.15 M Sodium Chloride, pH 7.2
Emission Wavelength
575 nm
Stabilizer
10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free
Preparation and Storage
Store vial at 4 degree C prior to opening. DO NOT FREEZE. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product is stable at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. Expiration date for properly stored material is six (6) months from date of opening.
Related Product Information for IgG secondary antibody
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins.
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Product Notes
The IgG (Catalog #AAA417974) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Phycoerythrin Conjugated Affinity Purified anti-Rabbit IgG [H&L] [Goat] reacts with Rabbit and may cross-react with other species as described in the data sheet. AAA Biotech's IgG [H&L] can be used in a range of immunoassay formats including, but not limited to, Immunomicroscopy, Flow Cytometry (FC/FACS), Other antibody based fluorescent assays requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications. Researchers should empirically determine the suitability of the IgG for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG [H&L], Secondary Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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