Western Blot (WB)
(WB: CHO cells transfected with hCAR were probed with MBS6120710 (0.2ug/ml). Arrow indicates CAR (46kD).)
Mouse CAR Monoclonal Antibody | anti-CAR antibody
CAR (Coxsackie Adenovirus Receptor) (Azide and Glycerol Free)
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
CAR; Monoclonal Antibody; CAR (Coxsackie Adenovirus Receptor) (Azide and Glycerol Free); anti-CAR antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
2T6
Specificity
Recognizes human CAR at ~46kD.
Species Crossreactivity: Mouse and Rat
Species Crossreactivity: Mouse and Rat
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in Tris-glycine. Azide and Glycerol free. Also available with sodium azide.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-CAR antibody
Immunocytochemistry (ICC), Western Blot (WB)
Application Notes
ICC: 1-2ug/ml shows positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.
WB: 1:500 detects CAR on 10ug of mouse small intestine lysates. Mouse small intestine lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with (HRP) goat anti-mouse and a chemiluminescence detection system.
WB: 1:500 detects CAR on 10ug of mouse small intestine lysates. Mouse small intestine lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunogen
Native human coxsackie virus adenovirus receptor protein.
Positive Control
Lung tissue
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WB: CHO cells transfected with hCAR were probed with MBS6120710 (0.2ug/ml). Arrow indicates CAR (46kD).)
Western Blot (WB)
(WB: CHO cells transfected with hCAR were probed with MBS6120710 (0.2ug/ml). Arrow indicates CAR (46kD).)
Product Categories/Family for anti-CAR antibody
References
1. Pickles, R., et. al., J. Virology 74: 6050-6057 (2000). 2. Tomko, R.P., et. al., PNAS 94: 3352-3356 (1997). 3. Bergelson, J., et. al., Science 275: 1320-1323 (1997). 4. Hsu, K.L., et. al., J. Virology 62: 1647-1652 (1988).
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Product Notes
The CAR (Catalog #AAA6120710) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CAR (Coxsackie Adenovirus Receptor) (Azide and Glycerol Free) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's CAR can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Western Blot (WB). ICC: 1-2ug/ml shows positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X. WB: 1:500 detects CAR on 10ug of mouse small intestine lysates. Mouse small intestine lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with (HRP) goat anti-mouse and a chemiluminescence detection system. Researchers should empirically determine the suitability of the CAR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CAR, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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