Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
2006 results for "Goat IgG Isotype Control" - showing 500-550
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-TGF-alpha antibody tAb2 (Ab00614) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of tAb2 (Ab00614-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
TGFalpha, Monoclonal Recombinant Antibody (Cat# AAA488330)
Full Name
Anti-TGFalpha [tAb2]
Gene Names
TGFA; TFGA
Reactivity
Human
Applications
XRC, ITC, DB, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was mouse IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
EGFR, Monoclonal Antibody (Cat# AAA9231961)
Full Name
EGFR Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808801)
Full Name
Methylglyoxal Antibody, Clone 9E7: Biotin
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,255/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
4-Hydroxy-2-hexenal, Monoclonal Antibody (Cat# AAA808684)
Full Name
4-Hydroxy-2-hexenal Antibody, Clone 6F10: ATTO 488
Reactivity
Species Independent
Applications
WB, ICC, IF, EIA, FC/FACS
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,275/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808889)
Full Name
Dityrosine Antibody, Clone 10A6: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 (MBS488455) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488455, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488455)
Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808658)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of SLK using anti-SLK antibody (MBS1750680).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,Lane 2: human PANC-1 whole cell lysates,Lane 3: human 22RV1 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLK antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLK at approximately 200KD. The expected band size for SLK is at 143KD. )
SLK, Polyclonal Antibody (Cat# AAA1750680)
Full Name
Anti-SLK antibody
Gene Names
SLK; LOSK; STK2; se20-9; bA16H23.1
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from Raji,Ramos,Daudi cell line (from left to right), using CHRNA9 Antibody (N-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
CHRNA9, Polyclonal Antibody (Cat# AAA9231859)
Full Name
CHRNA9 Antibody (N-term)
Gene Names
CHRNA9; NACHRA9; HSA243342
Reactivity
Human
Predicted: Rat
Predicted: Rat
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(All lanes : PABP1 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 71 KDa Observed band size : 71 KDa Blocking/Dilution buffer : 1x TBST.)
PABP1, Monoclonal Antibody (Cat# AAA435141)
Full Name
PABP1 Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot detection of MECT1 / Torc1 in Hela and Jurkat lysates using MECT1 / Torc1 mouse mAb (1:1000 diluted).Predicted band size: 78KDa.Observed band size: 78KDa.)
MECT1/Torc1, Monoclonal Antibody (Cat# AAA475107)
Full Name
Anti-MECT1/Torc1 Mouse mAb
Gene Names
CRTC1; MECT1; TORC1; TORC-1; WAMTP1
Reactivity
Human
Applications
WB, FC/FACS, ICC, IP
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB)
(Figure 1. Western blot analysis of Cyclin B1 using anti-Cyclin B1 antibody (MBS176782).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.lane 1: recombinant human cyclin b1 protein 0.5ng.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin B1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cyclin B1 at approximately 39KD. The expected band size for Cyclin B1 is at 39KD. )
Cyclin B1, Polyclonal Antibody (Cat# AAA176782)
Full Name
Anti-Cyclin B1 Antibody
Gene Names
CCNB1; CCNB
Reactivity
Human
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808673)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5E8: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9F11. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808820)
Full Name
Methylglyoxal Antibody, Clone 9F11: FITC
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,235/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808607)
Full Name
Acrolein Antibody, Clone 10A10: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808605)
Full Name
Acrolein Antibody, Clone 10A10: HRP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$545/0.1 mg | $2,215/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of D2AP using anti-D2AP antibody (M01756). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysate, Lane 2: human A431 whole cell lysate, Lane 3: human 293T whole cell lysate, Lane 4: human U20S whole cell lysate, Lane 5: human HL-60 whole cell lysate, Lane 6: human MCF-7 whole cell lysate, Lane 7: human Hela whole cell lysate, Lane 8: human PANC-1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-D2AP antigen affinity purified monoclonal antibody at 0.5 ug/ml overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)
CD2AP, Monoclonal Antibody (Cat# AAA1752130)
Full Name
Anti-CD2AP Antibody (monoclonal, 5F8)
Gene Names
CD2AP; CMS
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PDE4D using anti-PDE4D antibody (MBS1750572).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDE4D antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDE4D at approximately 91KD. The expected band size for PDE4D is at 91KD. )
PDE4D, Polyclonal Antibody (Cat# AAA1750572)
Full Name
Anti-PDE4D Picoband antibody
Gene Names
PDE4D; DPDE3; PDE43; STRK1; ACRDYS2; HSPDE4D; PDE4DN2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD5 and Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD45RC detected with Goat anti Mouse IgG1:RPE . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
CD45RC, Monoclonal Antibody (Cat# AAA225691)
Full Name
Mouse Anti Pig CD45RC: RPE
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$630/100 Tests | $2,680/5x100 Tests
Western Blot (WB)
(All lanes : Anti-CD74 Antibody at 1:1000 dilution Lane 1: Daudi whole cell lysates Lane 2: Raji whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG (H+L),Peroxidase conjugated at 1/5,000 dilution Predicted band size : 34 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
CD74, Monoclonal Antibody (Cat# AAA9232065)
Full Name
CD74 Antibody
Gene Names
CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Nitrotryptophan, Monoclonal Antibody (Cat# AAA808835)
Full Name
Nitrotryptophan Antibody, Clone 2D12: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Nitrotryptophan Monoclonal Antibody, Clone 2D12. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Nitrotryptophan Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Nitrotryptophan Antibody. (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Nitrotryptophan, Monoclonal Antibody (Cat# AAA808841)
Full Name
Nitrotryptophan Antibody, Clone 2D12: PerCP
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,270/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 2H2. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808575)
Full Name
Acrolein Antibody, Clone 2H2: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Testing Data
(Figure A. FITC conjugated Mouse anti Pig CD5 and Mouse IgG1 isotype control detected with Goat anti Mouse IgG1:RPE . Figure B. FITC conjugated Mouse anti Pig CD5 and Mouse anti Pig CD45RC detected with Goat anti Mouse IgG1:RPE . All experiments performed on red cell lysed porcine blood gated on lymphocytes in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.)
CD45RC, Monoclonal Antibody (Cat# AAA225690)
Full Name
Mouse Anti Pig CD45RC
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Testing Data
(1:1000 dilution of this antibody detected CHD3 on 40ug of cell lysates)
CHD3, Monoclonal Antibody (Cat# AAA9400325)
Full Name
CHD3 Monoclonal Antibody
Gene Names
CHD3; ZFH; Mi-2a; Mi2-ALPHA
Reactivity
Human, Porcine, Dog, Mouse, Rat
Applications
WB, ICC, IHC, FC/FACS
Purity
Purified by antigen-affinity chromatography.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
SDS-Page
(SDS-PAGE Analysis Purified PD-L2 Mouse Monoclonal Antibody (Z64P2D3*H4). Confirmation of Purity and Integrity of Antibody.)
PD-L2/PDCD1LG2/CD273, Monoclonal Antibody (Cat# AAA4381354)
Full Name
PD-L2/PDCD1LG2/CD273
Reactivity
Human
Applications
FC/FACS
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Methylglyoxal Monoclonal Antibody, Clone 9E7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Methylglyoxal Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Methylglyoxal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Methylglyoxal, Monoclonal Antibody (Cat# AAA808806)
Full Name
Methylglyoxal Antibody, Clone 9E7: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of HOXA11 using anti-HOXA11 antibody (MBS177415). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. lane 1: Recombinant Human HOXA11 Protein 0.5ng. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HOXA11 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HOXA11 at approximately 38KD. The expected band size for HOXA11 is at 38KD. )
Homeobox protein Hox-A11, Polyclonal Antibody (Cat# AAA177415)
Full Name
Anti-HOXA11 Antibody
Gene Names
HOXA11; HOX1; HOX1I
Reactivity
Human, Mouse, Rat. No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Dityrosine Monoclonal Antibody, Clone 10A6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Dityrosine Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Localization: Cytoplasmic. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Dityrosine Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Dityrosine, Monoclonal Antibody (Cat# AAA808896)
Full Name
Dityrosine Antibody, Clone 10A6: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CHRNA3 using anti-CHRNA3 antibody (MBS1750735).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,Lane 2: human MDA-MB-453 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human SK-OV-3 whole cell lysates,Lane 6: human PANC-1 whole cell lysates,Lane 7: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHRNA3 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CHRNA3 at approximately 60KD. The expected band size for CHRNA3 is at 57KD. )
CHRNA3, Polyclonal Antibody (Cat# AAA1750735)
Full Name
Anti-CHRNA3 Picoband Antibody
Gene Names
CHRNA3; LNCR2; PAOD2; NACHRA3
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from human heart and skeletal muscle tissue lysate (from left to right), using FABP3 Antibody. It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
FABP3, Polyclonal Antibody (Cat# AAA9231617)
Full Name
FABP3 Antibody
Gene Names
FABP3; MDGI; FABP11; H-FABP; M-FABP; O-FABP
Reactivity
Human
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9207717 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
FBXL17 Isoform 2, Polyclonal Antibody (Cat# AAA9207717)
Full Name
FBXL17 Isoform 2 Antibody (C-term)
Gene Names
FBXL17; Fbl17; Fbx13; FBXO13
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of HepG2 cells using (Mouse) Epcam Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). It was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)
Epcam, Polyclonal Antibody (Cat# AAA9232059)
Full Name
(Mouse) Epcam Antibody (C-term)
Gene Names
Epcam; EGP; Ly74; gp40; CD326; EGP-2; TROP1; Egp314; Ep-CAM; EpCAM1; Tacsd1; GA733-2; Tacstd1
Reactivity
Human, Mouse
Applications
WB, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. brain section using PIP4K2A Antibody (C-term). MBS9202730 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
PIP4K2A, Polyclonal Antibody (Cat# AAA9202730)
Full Name
PIP4K2A Antibody (C-term)
Gene Names
PIP4K2A; PIPK; PI5P4KA; PIP5K2A; PIP5KIIA; PIP5KII-alpha
Reactivity
Human (Predicted Reactivity: Pig)
Applications
EIA, IHC, FC/FACS, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Flow cytometric analysis of 2% paraformaldehyde-fixed THP1 (Human acute monocytic leukemia cell line) cells labeling CSTF3 with M25222 at 1/200 dilution (red) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
CSTF3, Monoclonal Antibody (Cat# AAA435130)
Full Name
CSTF3 (734) Mouse mAb
Gene Names
CSTF3; CSTF-77
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data
(Immunocytochemistry stain of Hela using TORC1 antibody (1:300).)
TORC1, Monoclonal Antibody (Cat# AAA857757)
Full Name
TORC1 (MECT1) Antibody
Gene Names
CRTC1; MECT1; TORC1; TORC-1; WAMTP1
Reactivity
Human
Applications
WB, FC/FACS, ICC, IP
Purity
Affinity purified
Pricing
$330/0.1 mg | $1,425/5x0.1 mg | $570/0.1 mL (Biotin) | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(All lanes : IF4A3 Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 47 KDa Observed band size : 47 KDa Blocking/Dilution buffer : 1x TBST.)
IF4A3, Monoclonal Antibody (Cat# AAA435125)
Full Name
IF4A3 Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-STRADA Antibody (C-term) at 1:1000 dilutionLane 1: 293T whole cell lysatesLane 2: human brain lysatesLane 3: mouse brain lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilutionPredicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
STRADA, Polyclonal Antibody (Cat# AAA9231944)
Full Name
STRADA Antibody (C-term)
Gene Names
STRADA; LYK5; PMSE; Stlk; STRAD; NY-BR-96; STRAD alpha
Reactivity
Human, Mouse
Applications
WB, IHC, IF, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with EpCAM Rabbit Recombinant Monoclonal Antibody (EGP40/2571R).)
Ep-CAM/CD326, Antibody (Cat# AAA4381073)
Full Name
Ep-CAM/CD326 (Extracellular Domain) (Epithelial Marker)
Gene Names
EPCAM; ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; EGP314; HNPCC8; TACSTD1
Reactivity
Human
Applications
FC/FACS, IHC
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5E8. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808680)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5E8: RPE
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$555/0.1 mg | $2,260/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Acrolein Monoclonal Antibody, Clone 10A10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Acrolein Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Acrolein Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Acrolein, Monoclonal Antibody (Cat# AAA808593)
Full Name
Acrolein Antibody, Clone 10A10: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808647)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: ATTO 390
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$560/0.1 mg | $2,280/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody, Clone 5D9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Hexanoyl-Lysine adduct Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Hexanoyl-Lysine adduct Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Hexanoyl-Lysine adduct, Monoclonal Antibody (Cat# AAA808655)
Full Name
Hexanoyl-Lysine adduct Antibody, Clone 5D9: Alkaline Phosphatase
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$550/0.1 mg | $2,245/5x0.1 mg
Immunocytochemistry/Immunofluorescence (ICC/IF)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Malondialdehyde Monoclonal Antibody, Clone 6H6. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-Malondialdehyde Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 uM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alex Fluor 633 F-Actin stain. (E,F) Malondialdehyde Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.)
Malondialdehyde, Monoclonal Antibody (Cat# AAA808754)
Full Name
Malondialdehyde Antibody, Clone 6H6
Reactivity
Species Independent
Applications
WB, ICC, IF, FC/FACS, EIA
Purity
Protein G Purified
Pricing
$505/0.1 mg | $2,050/5x0.1 mg
Testing Data
(Published customer image: Ex vivo recognition of yeast particles and live fungi by inflammatory cells. A) Representative flow-cytometric analysis, gated on Ly-6G+ neutrophils, after coincubation of BIOgel-elicited inflammatory cells from wild type or dectin-1-deficient (Clec7a-/-) 129S6/SvEv interaction with serum-opsonized or non-opsonized zymosan. Positive staining for the A405-labelled zymosan identifies the neutrophils that are associated zymosan and only these cells exhibit conversion of APF, the ROS reporter. B) Representsative flow-cytometric analysis of CD11b and dectin-1 expression by inflammatory neutrophils and monocyte/M˜. Data represents specific receptor staining (shaded histograms) and isotype control staining (bold lines). Data representative of 2 independent experiments and consistent with previous experiments with thioglycollate. C) Inflammatory cells were loaded with APF and then incubated with serum-opsonized or non-opsonized A405-labelled zymosan or Pacific Orange-labelled C. albicans for 15 minutes. After this time the association of the inflammatory cells with zymosan was measured by flow-cytometry (upper panels) and in those cells that were interacting with zymosan the evidence for fluorescent conversion of APF was also quantified (lower panels). Data is derived from three independent experiments and the data derived from the use of dectin-1-deficient cells is shown relative to wild type cells (100%) as mean+/-95% confidence interval (raw representative data from one of the 3 independent experiments are shown in the Figure S1). The impact of complement osponization (˜C') and the use of different fungal particle used (˜F') were assessed by Two-way ANOVA (˜I' = Interaction statistic). Samples in which the 95% confidence intervals do not overlap with the mean wildtype are specific indicated with a # symbol. Differences in impairment of response observed with dectin-1-deficient cells were further analysed by Bonferroni post-tests. P values derived from individual Bonferroni post-tests are indicated with bracketed pairs of samples.From: McDonald JU, Rosas M, Brown GD, Jones SA, Taylor PR (2012) Differential Dependencies of Monocytes and Neutrophils on Dectin-1, Dectin-2 and Complement for the Recognition of Fungal Particles in Inflammation. PLoS ONE 7(9): e45781.)
DECTIN-1, Monoclonal Antibody (Cat# AAA215799)
Full Name
RAT ANTI MOUSE DECTIN-1:RPE
Gene Names
Clec7a; BGR; beta-GR; Clecsf12
Applications
FC/FACS
Pricing
$255/25 Tests | $995/5x25 Tests
Testing Data
(Staining of porcine peripheral blood lymphocytes with Mouse anti Porcine CD46 .)
CD46, Monoclonal Antibody (Cat# AAA225653)
Full Name
Mouse Anti Pig CD46
Gene Names
CD46; MCP
Reactivity
Pig
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$355/0.1 mg | $1,435/5x0.1 mg
Testing Data
(1:1000 dilution of this antibody detected ACLY on 40ug of cell lysates)
ATP-Citrate Lyase, Monoclonal Antibody (Cat# AAA9400155)
Full Name
ATP-Citrate Lyase(C-term) Monoclonal Antibody
Gene Names
ACLY; ACL; ATPCL; CLATP
Reactivity
Human, Mouse, Rat, Porcine
Applications
WB, ICC, FC/FACS
Purity
Purified by antigen-affinity chromatography.
Pricing
$305/0.1 mL | $1,230/5x0.1 mL
SDS-Page
(SDS-PAGE Analysis Purified CD8 Mouse Monoclonal Antibody (UCHT4). Confirmation of Purity and Integrity of Antibody.)
CD8A, Monoclonal Antibody (Cat# AAA4381399)
Full Name
CD8A (Cytotoxic- & Suppressor T-Cell Marker)
Gene Names
CD8A; CD8; p32; Leu2
Reactivity
Human
Applications
FC/FACS
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-BAZ1B monoclonal antibody. )
BAZ1B, Monoclonal Antibody (Cat# AAA120064)
Full Name
Mouse monoclonal antibody Anti-Human BAZ1B
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
Western Blot (WB)
(Western blot analysis of lysates from mouse NIH/3T3,BA/F3,mouse C2C12,rat C6 cell line (from left to right),using Mouse Mapk3 Antibody (C-term). It was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
Mapk3, Polyclonal Antibody (Cat# AAA9231614)
Full Name
Mouse Mapk3 Antibody (C-term)
Gene Names
Mapk3; p44; Erk1; Ert2; Mnk1; Erk-1; Esrk1; Prkm3; Mtap2k; p44erk1; p44mapk
Reactivity
Mouse, Rat
Predicted: Human
Predicted: Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
