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Flow Cytometry (FC/FACS) (Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9207717 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Rabbit anti-Human FBXL17 Isoform 2 Polyclonal Antibody | anti-FBXL17 antibody

FBXL17 Isoform 2 Antibody (C-term)

Gene Names
FBXL17; Fbl17; Fbx13; FBXO13
Reactivity
Human
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
FBXL17 Isoform 2; Polyclonal Antibody; FBXL17 Isoform 2 Antibody (C-term); F-box/LRR-repeat protein 17; F-box and leucine-rich repeat protein 17; F-box only protein 13; FBXL17; FBL17; FBX13; FBXO13; anti-FBXL17 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This FBXL17 Isoform 2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 262-297 amino acids from the C-terminal region of human FBXL17 Isoform 2.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
701
Applicable Applications for anti-FBXL17 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
Application Notes
FC~~1:25
IHC~~1:25
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9207717 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of MCF-7 cells using FBXL17 Isoform 2 Antibody (C-term)(green) compared to an isotype control of rabbit IgG(blue). MBS9207717 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. kidney section using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. kidney section using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded R. kidney section using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded R. kidney section using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded M. kidney section using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded M. kidney section using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

Western Blot (WB)

(Western blot analysis of lysate from MCF-7 cell line, using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)

Western Blot (WB) (Western blot analysis of lysate from MCF-7 cell line, using FBXL17 Isoform 2 Antibody (C-term). MBS9207717 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.)
Related Product Information for anti-FBXL17 antibody
Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex (By similarity).
Product Categories/Family for anti-FBXL17 antibody
References
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Schmutz J.,et al.Nature 431:268-274(2004).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
75695
NCBI Official Full Name
F-box/LRR-repeat protein 17
NCBI Official Synonym Full Names
F-box and leucine-rich repeat protein 17
NCBI Official Symbol
FBXL17
NCBI Official Synonym Symbols
Fbl17; Fbx13; FBXO13
NCBI Protein Information
F-box/LRR-repeat protein 17
UniProt Protein Name
F-box/LRR-repeat protein 17
Protein Family
UniProt Gene Name
FBXL17
UniProt Synonym Gene Names
FBL17; FBX13; FBXO13
UniProt Entry Name
FXL17_HUMAN

NCBI Description

Members of the F-box protein family, such as FBXL17, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]

Uniprot Description

FBXL17: Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 5q21.3

Research Articles on FBXL17

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Product Notes

The FBXL17 fbxl17 (Catalog #AAA9207717) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FBXL17 Isoform 2 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FBXL17 Isoform 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC). FC~~1:25 IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the FBXL17 fbxl17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FBXL17 Isoform 2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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