Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "ECL" - showing 1-50
Western Blot (WB)
(The recombinant proteins and tissue lysate were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Crystallin alpha A antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. This antibody is not shown cross-activity about Crystallin alpha B.A : Recombinant human Crystallin alpha A proteinB : Recombinant human Crystallin alpha B proteinC : Mouse eye tissue lysate)
A crystallin A, Monoclonal Antibody (Cat# AAA200007)
Full Name
A crystallin A antibody
Gene Names
CRYAA; CRYA1; HSPB4; CTRCT9
Reactivity
Human
Applications
EIA, WB
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of 293 and HeLa cells, using Phospho-AKT1-T450 antibody at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 1min.)
AKT1-T450, Antibody (Cat# AAA125441)
Full Name
Phospho-AKT1-T450 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$260/0.05 mL | $385/0.1 mL | $570/0.2 mL
Western Blot (WB)
(The recombinant human synuclein family (alpha-,beta- and gamma-) (each 20ng), Mouse brain and Rat brain (30ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human alpha,beta-synuclein (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Alpha, Beta - Synuclein, Monoclonal Antibody (Cat# AAA200043)
Full Name
Alpha, Beta - Synuclein antibody
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AKT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
AKT1, Polyclonal Antibody (Cat# AAA9125011)
Full Name
AKT1 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-AKT1-T308 antibody at 1:2000 dilution. HeLa cells were treated by 10% FBS for after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Enhanced Kit.Exposure time: 90s.)
AKT1-T308, Antibody (Cat# AAA128342)
Full Name
Phospho-AKT1-T308 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H4-K20 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
H4K20me3, Antibody (Cat# AAA126181)
Full Name
Histone H4K20me3 Polyclonal Antibody
Gene Names
HIST2H4A; H4; H4/n; H4F2; H4FN; FO108; HIST2H4
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using AKT1 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
AKT1, Polyclonal Antibody (Cat# AAA9127855)
Full Name
AKT1 Polyclonal Antibody
Gene Names
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL
Western Blot (WB)
(The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate)
Adiponectin, Monoclonal Antibody (Cat# AAA200027)
Full Name
Adiponectin antibody
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Reactivity
Human, Mouse
Applications
EIA, WB, FC/FACS, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(The Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant hGH (21-217aa) Lane 2.: 20kDa hGH (27-202aa))
Growth Hormone, Monoclonal Antibody (Cat# AAA200008)
Full Name
Growth Hormone Antibody
Gene Names
GH1; GH; GHN; GH-N; GHB5; hGH-N; IGHD1B
Reactivity
Human, Mouse
Applications
EIA, WB
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(The recombinant human synuclein family (alpha-, beta- and gamma-) and alpha-synuclein domains (1-60, 1-95, 61-140 and 96-140) proteins were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-alpha-Synuclein (61-95 aa) antibody (1:1,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Alpha-Synuclein, Monoclonal Antibody (Cat# AAA200019)
Full Name
Alpha-Synuclein
Gene Names
SNCA; PD1; NACP; PARK1; PARK4
Reactivity
Human
Applications
EIA, WB, Recombinant protein,Cell lysate
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(The recombinant proteins and mouse liver lysates were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human adiponectin antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Arrows indicate the oligomer and monomer of mAcrp30 protein in mouse liver. * gAcrp30 : globular domain of adiponectin)
Adiponectin, Monoclonal Antibody (Cat# AAA200018)
Full Name
Adiponectin antibody
Gene Names
ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
Reactivity
Human, Mouse
Applications
EIA, WB, FC/FACS
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CD2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
CD2, Polyclonal Antibody (Cat# AAA9127010)
Full Name
CD2
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human
Applications
WB, IHC
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(The Recombinant Human HSP90 (50ng) and Cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human HSP90antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection systemLane 1. : Hsp90 recombinant protein Lane 2. : 293T cell lysate Lane 3. : HeLa cell lysate Lane 4. : HepG2 cell lysate)
Hsp90, Monoclonal Antibody (Cat# AAA200047)
Full Name
Hsp90 antibody
Gene Names
HSP90AA1; EL52; HSPN; LAP2; HSP86; HSPC1; HSPCA; Hsp89; Hsp90; LAP-2; HSP89A; HSP90A; HSP90N; Hsp103; HSPCAL1; HSPCAL4; HEL-S-65p
Reactivity
Human,
Applications
EIA, Cell lysate, IHC
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of rat brain, using PELI1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
PELI1, Polyclonal Antibody (Cat# AAA9128859)
Full Name
PELI1 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Cell lysates of NIH-3T3, HepG2, Jurkat, HeLa and SH-SY5Y (each 40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CRP (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. )
CRP, Monoclonal Antibody (Cat# AAA200044)
Full Name
CRP antibody
Gene Names
CRP; PTX1
Reactivity
Human, Mouse
Applications
EIA, WB, ICC, IF, FC/FACS
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
Histidine Decarboxylase, Polyclonal Antibody (Cat# AAA535374)
Full Name
Histidine Decarboxylase antibody
Reactivity
Dog, Guinea Pig, Human, Mouse, Rat
Applications
IF
Purity
Whole antiserum
Pricing
$1,010/0.05 mL | $4,535/5x0.05 mL
Matrix Metalloproteinase-7, Active Protein (Cat# AAA142412)
Full Name
Recombinant Human Matrix Metalloproteinase-7
Gene Names
MMP7; MMP-7; MPSL1; PUMP-1
Purity
Greater than 95.0% as determined by(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.02 mg | $5,300/1 mg | $180/0.005 mg | $23,620/5x1 mg
Testing Data
Epidermal Growth Factor Receptor (EGFR), phospho-Tyr 845 + Positive Control, Monoclonal Antibody (Cat# AAA311323)
Full Name
MAb to EGFR pTyr 845
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61
Reactivity
Human
Applications
EIA/ELISA, Western Blot
Purity
Thiophilic adsorption and size exclusion chromatography
Pricing
$910/0.1 mg | $3,945/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NeuN antibody (MBS125033) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
NeuN, Polyclonal Antibody (Cat# AAA125033)
Full Name
NeuN Polyclonal Antibody
Gene Names
RBFOX3; FOX3; NEUN; FOX-3; HRNBP3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using IRAK4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IRAK4, Polyclonal Antibody (Cat# AAA9125999)
Full Name
IRAK4 Polyclonal Antibody
Gene Names
IRAK4; IPD1; REN64; IRAK-4; NY-REN-64
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200
) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
H3K9me3, Antibody (Cat# AAA126185)
Full Name
Histone H3K9me3 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
DB, WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of Mouse spleen, using (MBS126857) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
CAMP, Polyclonal Antibody (Cat# AAA126857)
Full Name
CAMP Polyclonal Antibody
Gene Names
CAMP; LL37; CAP18; CRAMP; HSD26; CAP-18; FALL39; FALL-39
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using MMP9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) ( MBS128200 ) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)
MMP9, Polyclonal Antibody (Cat# AAA125192)
Full Name
MMP9 Polyclonal Antibody
Gene Names
MMP9; GELB; CLG4B; MMP-9; MANDP2
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using STAMBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
STAMBP, Polyclonal Antibody (Cat# AAA9126436)
Full Name
STAMBP Polyclonal Antibody
Gene Names
STAMBP; AMSH; MICCAP
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TFEB antibody (MBS9125929) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
TFEB, Polyclonal Antibody (Cat# AAA9125929)
Full Name
TFEB Polyclonal Antibody
Gene Names
TFEB; TCFEB; BHLHE35; ALPHATFEB
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of CD68 using anti- CD68 antibody Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Spleen Tissue Lysate Lane 2: Mouse Spleen Tissue Lysate Lane 3: Mouse RAW246.7 Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD68 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.)
CD68, Polyclonal Antibody (Cat# AAA175328)
Full Name
Anti-CD68 antibody
Gene Names
Cd68; Lamp4; gp110; Scard1
Reactivity
Mouse, Rat
Applications
WB, IHC, IHC
Purity
Immunogen affinity purified.
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(The Mouse tissue lysate (40ug) was resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human FABP7 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: Testis tissue lysate)
FABP7, Monoclonal Antibody (Cat# AAA200130)
Full Name
FABP7 antibody
Gene Names
FABP7; MRG; BLBP; FABPB; B-FABP
Reactivity
Human, Mouse
Applications
EIA, WB, ICC, IF
Purity
By protein-G affinity chromatography
Pricing
$260/0.05 mL | $345/0.1 mL | $1,300/5x0.1 mL
CYTOSINE DEAMINASE, Polyclonal Antibody (Cat# AAA220797)
Full Name
SHEEP ANTI YEAST CYTOSINE DEAMINASE
Applications
EIA, WB
Pricing
$345/1 mL | $1,405/5x1 mL
Hypersensitive ECL Chemiluminescent Substrate, Reagent (Cat# AAA355702)
Full Name
Hypersensitive ECL Chemiluminescent Substrate
Pricing
$200/100 mL | $630/5x100 mL
Western Blot (WB)
(Western blot analysis of extracts of HeLa cells, using Phospho-STAT3-Y705 antibody (MBS125978) at 1:500 dilution.HeLa cells were treated by IFN-α (100 ng/ml) at 37°C for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.)
Stat3-Y705, Antibody (Cat# AAA125978)
Full Name
Phospho-Stat3-Y705 Polyclonal Antibody
Gene Names
STAT3; APRF; HIES; ADMIO
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Figure 1. Western blot analysis of ABCB4 using anti-ABCB4 antibody (MBS177897).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: MCF-7 Whole Cell Lysate,Lane 2: SW620 Whole Cell Lysate,Lane 3: 22RV1 Whole Cell Lysate,Lane 4: SKOV Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCB4 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ABCB4 at approximately 142KD. The expected band size for ABCB4 is at 142KD. )
ABCB4, Polyclonal Antibody (Cat# AAA177897)
Full Name
Anti-ABCB4 Antibody
Gene Names
ABCB4; GBD1; ICP3; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-P, IF/ICC, FC/FACS
Purity
Immunogen Affinity Purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Exodus-2, Active Protein (Cat# AAA142039)
Full Name
Recombinant Human Exodus-2 (CCL21)
Gene Names
CCL21; ECL; SLC; CKb9; TCA4; 6Ckine; SCYA21
Purity
Greater than 97.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Pricing
$250/0.02 mg | $2,830/1 mg | $180/0.005 mg | $12,520/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts of normal 293T cells and 293T transfected with His-CEND1 protein,using His-Tag antibody (MBS125007) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) ( MBS128212 ) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 120s.)
His-Tag, Antibody (Cat# AAA125007)
Full Name
His-Tag Monoclonal Antibody
Applications
WB, IP
Purity
Affinity Purification
Pricing
$175/0.05 mL | $215/0.1 mL | $285/0.2 mL | $1,130/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using C3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
C3, Polyclonal Antibody (Cat# AAA9125960)
Full Name
C3
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using TriMethyl-Histone H3-K27 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
H3K27me3, Antibody (Cat# AAA126239)
Full Name
Histone H3K27me3 Polyclonal Antibody
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Reactivity
Human, Mouse, Rat, Other (Wide Range)
Applications
WB, IHC, IF, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using HMGCR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
HMGCR, Polyclonal Antibody (Cat# AAA127489)
Full Name
HMGCR Polyclonal Antibody
Gene Names
HMGCR; LDLCQ3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot
(Western Blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody MBS9126034 at 1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1uM) at 37°C for 18 hours.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1s.)
H3-K9, Antibody (Cat# AAA9126034)
Full Name
Acetyl-Histone H3-K9
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using CCAR2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
CCAR2, Polyclonal Antibody (Cat# AAA9125196)
Full Name
CCAR2 Polyclonal Antibody
Gene Names
CCAR2; DBC1; DBC-1; NET35; p30DBC; p30 DBC; KIAA1967
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Western Blot (WB)
(Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)
Erk1/2-T202/Y204, Antibody (Cat# AAA9127838)
Full Name
Phospho-Erk1/2-T202/Y204 Polyclonal Antibody
Gene Names
MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse pancreas, using FOXN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
FOXN3, Antibody (Cat# AAA9135025)
Full Name
FOXN3 Polyclonal Antibody
Gene Names
FOXN3; CHES1; PRO1635; C14orf116
Reactivity
Mouse
Applications
WB
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of A-549 cells, using TLR4 antibody (MBS129143) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5min.)
TLR4, Polyclonal Antibody (Cat# AAA129143)
Full Name
TLR4
Gene Names
TLR4; TOLL; CD284; TLR-4; ARMD10
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Hypersensitive ECL Chemiluminescent Substrate, Reagent (Cat# AAA355689)
Full Name
Hypersensitive ECL Chemiluminescent Substrate (Concentrated)
Pricing
$205/10 mL | $660/5x10 mL
Western Blot (WB)
(Western blot analysis of extracts from normal (control) and STAT3 knockout (KO) HeLa cells, using STAT3 antibody (A1192) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s)
STAT3, Polyclonal Antibody (Cat# AAA127578)
Full Name
STAT3 Polyclonal Antibody
Gene Names
STAT3; APRF; HIES
Applications
WB, IHC, IF
Purity
Affinity purification
Pricing
$210/0.02 mL | $265/0.05 mL | $380/0.1 mL | $540/0.2 mL | $2,285/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts ofvarious cell lines, using GPX4 antibody (MBS126523) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
GPX4, Polyclonal Antibody (Cat# AAA126523)
Full Name
GPX4 Polyclonal Antibody
Gene Names
GPX4; MCSP; GPx-4; PHGPx; snGPx; GSHPx-4; snPHGPx
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of NIH/3T3 cells, using Acetyl-Histone H3-K56 antibody (MBS9126071) at 1:1000 dilution.NIH/3T3 cells were treated by TSA (1 uM) at 37°C for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 180s)
H3-K56, Antibody (Cat# AAA9126071)
Full Name
Acetyl-Histone H3-K56
Gene Names
HIST3H3; H3t; H3.4; H3/g; H3FT
Applications
WB, IHC, IF, IP
Purity
Affinity purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant FOXP3, Human.)
Forkhead Box Protein P3, Polyclonal Antibody (Cat# AAA2032402)
Full Name
Forkhead Box Protein P3 (FOXP3) Polyclonal Antibody
Gene Names
FOXP3; JM2; AIID; IPEX; PIDX; XPID; DIETER
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$160/0.1 mL | $195/0.2 mL | $320/0.5 mL | $405/1 mL | $1,160/5x1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ATF4 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
ATF4, Polyclonal Antibody (Cat# AAA125836)
Full Name
ATF4 Polyclonal Antibody
Gene Names
ATF4; CREB2; TXREB; CREB-2; TAXREB67
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ATG5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
ATG5, Polyclonal Antibody (Cat# AAA125660)
Full Name
ATG5 Polyclonal Antibody
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Testing Data
(Representative western blot (1:1000) using HeLa cell lysate.)
GAPDH, Monoclonal Antibody (Cat# AAA330060)
Full Name
Anti-GAPDH mAb
Gene Names
Gapdh; Gapd
Reactivity
Recognize GAPDH from human, mouse, rat, rabbit, BL-21 Bacteria, Sf9 Insect cells, and Saccharomyces cerevisiae Yeast. GAPDH from other species may also be detectable.
Applications
EIA, DB, WB
Pricing
$300/0.1 mg | $1,185/1 mg | $5,095/5x1 mg
