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Western Blot (WB) (The Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant hGH (21-217aa) Lane 2.: 20kDa hGH (27-202aa))

Mouse anti-Human, Mouse Growth Hormone Monoclonal Antibody | anti-GH1 antibody

Growth Hormone Antibody

Gene Names
GH1; GH; GHN; GH-N; GHB5; hGH-N; IGHD1B
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Neutralization Assay
Purity
By protein-G affinity chromatography
Synonyms
Growth Hormone; Monoclonal Antibody; Growth Hormone Antibody; GH1; GH; GH-N; Pituitary growth hormone; Growth hormone 1; Somatotropin; GHN; hGH-N; ; anti-GH1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2b, k
Clone Number
g3H5
Purity/Purification
By protein-G affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
217
Applicable Applications for anti-GH1 antibody
ELISA (EIA), Western Blot (WB), Neutralization
Application Notes
WB: 1:500 ~ 1,000.
Recommended starting dilution is 1:500.
Antigen Species
Human
Immunogen
Recombinant human growth hormone (27-217aa) purified from E. coli
Clone Note
Anti-human growth hormone mAb, clone g3H5, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant growth hormone.
Preparation and Storage
Can be stored at 4 degree C.
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant hGH (21-217aa) Lane 2.: 20kDa hGH (27-202aa))

Western Blot (WB) (The Recombinant protein (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Growth hormone antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant hGH (21-217aa) Lane 2.: 20kDa hGH (27-202aa))
Related Product Information for anti-GH1 antibody
Human growth hormone (hGH, somatotropin), a protein hormone from the anterior lobe of the pituitary gland, comprises a single polypeptide chain of 191 amino acid residues. The 20kDa variant form of human growth hormone (20kDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22kDa, 191 amino acid) by the deletion of amino acid residues 32-46. The growth hormone amino acid sequence varies considerably between species, and non-primate growth hormones have little activity in man. Main functions of hGH and 20kDa hGH are the stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues.
Product Categories/Family for anti-GH1 antibody
References
Wickens, et al., (1978) J. Biol Chem. 253 (7)2483-2495; Chang, et al., (1978) Nature. 275,617-624; Ullrich. et al., (1977) Science 196, 1313-1319.; Tsushima, et al., (2000) Endocr J. 47; S17-21; Horan, M. et al., (2003) Hum. Mutat.21 (4), 408-423

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
20,201 Da
NCBI Official Full Name
somatotropin isoform 1
NCBI Official Synonym Full Names
growth hormone 1
NCBI Official Symbol
GH1
NCBI Official Synonym Symbols
GH; GHN; GH-N; GHB5; hGH-N; IGHD1B
NCBI Protein Information
somatotropin
UniProt Protein Name
Somatotropin
Protein Family
UniProt Gene Name
GH1
UniProt Synonym Gene Names
GH; GH-N

NCBI Description

The protein encoded by this gene is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. [provided by RefSeq, Jul 2008]

Uniprot Description

Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.

Research Articles on GH1

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Product Notes

The GH1 gh1 (Catalog #AAA200008) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Growth Hormone Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Growth Hormone can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Neutralization. WB: 1:500 ~ 1,000. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the GH1 gh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Growth Hormone, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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