Rabbit Erk1/2-T202/Y204 Antibody | anti-MAPK1/ERK2, MAPK3/ERK1 antibody

Phospho-Erk1/2-T202/Y204 Polyclonal Antibody

Cat. Num. AAA9127838

• Gene Names: MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: Erk1/2-T202/Y204; Phospho-Erk1/2-T202/Y204 Polyclonal Antibody; Phospho-ERK1/2-T202/Y204: ERK; p38; p40; p41; ERK2; ERT1; MAPK2; PRKM1; PRKM2; P42MAPK; p41mapk; anti-MAPK1/ERK2; MAPK3/ERK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 379

• Applicable Applications for anti-MAPK1/ERK2, MAPK3/ERK1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500 - 1:2000
• Species: Human
• Route: Synthetic Peptide
• Immunogen: A phospho specific peptide corresponding to residues surrounding T202/Y204 of human Erk1/2
• Calculated Molecular Weight: 42,44KD
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of Jurkat cells, using Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 antibody at 1:2000 dilution. Jurkat cells were treated by PMA/TPA (200nM) for 10 minutes.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit.Exposure time: 5s.)

Related Product Information for anti-MAPK1/ERK2, MAPK3/ERK1 antibody

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.

Product Categories/Family for anti-MAPK1/ERK2, MAPK3/ERK1 antibody

Phosphorylation

NCBI and Uniprot Product Information

• NCBI GI #: 232066
• NCBI GeneID: 5595
• UniProt Accession #: P27361; Q8NHX1; A8CZ58; B0LPG3
• Molecular Weight: 40,088 Da
• NCBI Official Full Name: Mitogen-activated protein kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 3
• NCBI Official Symbol: MAPK3
• NCBI Official Synonym Symbols: ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 3; MAPK 1; MAP kinase isoform p44; insulin-stimulated MAP2 kinase; extracellular signal-related kinase 1; extracellular signal-regulated kinase 1; microtubule-associated protein 2 kinase
• UniProt Protein Name: Mitogen-activated protein kinase 3
• UniProt Gene Name: MAPK3
• UniProt Synonym Gene Names: ERK1; PRKM3; MAP kinase 3; MAPK 3; ERK-1; p44-MAPK
• UniProt Entry Name: MK03_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

ERK1: a serine/threonine kinase of the GMGC group that plays a critical role in the regulation of cell growth and differentiation. ERK1 (MAPK3) and ERK2 (MAPK1) play central roles in MAPK cascades and are activated by a wide variety of extracellular signals including growth and neurotrophic factors, cytokines, hormones and neurotransmitters. Depending on the cellular context, MAPK cascades mediate diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. MAPK cascades also plays a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. Activation of MAP kinases occurs through phosphorylation of threonine and tyrosine residues at the sequence T*EY* by upstream MAP kinase kinases, MEK1 and -2. Phosphorylation of both the threonine and tyrosine are required for activity. This phosphorylation causes dramatic conformational changes, which enable full activation and interaction of MAPK1/ERK2 with its substrates.

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: Golgi apparatus; focal adhesion; mitochondrion; early endosome; nuclear envelope; caveola; pseudopodium; cytosol; microtubule cytoskeleton; nucleoplasm; cytoskeleton; late endosome; nucleus

Molecular Function: MAP kinase activity; protein binding; phosphotyrosine binding; ATP binding; phosphatase binding

Biological Process: axon guidance; viral reproduction; activation of MAPKK activity; positive regulation of histone phosphorylation; nerve growth factor receptor signaling pathway; DNA damage induced protein phosphorylation; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; sensory perception of pain; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; BMP signaling pathway; toll-like receptor 5 signaling pathway; response to exogenous dsRNA; regulation of transcription factor activity; small GTPase mediated signal transduction; lipopolysaccharide-mediated signaling pathway; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; transcription from RNA polymerase I promoter; cell cycle; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; organ morphogenesis; cartilage development; Ras protein signal transduction; insulin receptor signaling pathway; toll-like receptor signaling pathway; innate immune response; positive regulation of histone acetylation; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; toll-like receptor 9 signaling pathway; transcription initiation from RNA polymerase I promoter; vascular endothelial growth factor receptor signaling pathway; blood coagulation; phosphorylation; regulation of cytoskeleton organization and biogenesis