<
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NeuN antibody (MBS125033) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Rabbit NeuN Polyclonal Antibody | anti-RBFOX3 antibody
NeuN Polyclonal Antibody
Gene Names
RBFOX3; FOX3; NEUN; FOX-3; HRNBP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
NeuN; Polyclonal Antibody; NeuN Polyclonal Antibody; RBFOX3: FLJ56884; FLJ58356; FOX3; HRNBP3; RBFOX3; NEUN; FOX-3; anti-RBFOX3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence Length
312
Applicable Applications for anti-RBFOX3 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF 1:50 - 1:200
IHC: 1:50 - 1:200
IF 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human NeuN (NP_001076044.1).
Preparation and Storage
Store at -20°C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NeuN antibody (MBS125033) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using NeuN antibody (MBS125033) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using NeuN antibody (MBS125033) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat brain using NeuN antibody (MBS125033) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using NeuN antibody (MBS125033) at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using NeuN antibody (MBS125033) at dilution of 1:100 (40x lens).)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using NeuN antibody (MBS125033) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of HeLa cells using NeuN antibody (MBS125033) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U-251MG cells using NeuN antibody (MBS125033) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of U-251MG cells using NeuN antibody (MBS125033) at dilution of 1:100. Blue: DAPI for nuclear staining.)
Related Product Information for anti-RBFOX3 antibody
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
Product Categories/Family for anti-RBFOX3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated MW: 33kDa/35kDa
Observed MW: 50kDa
Observed MW: 50kDa
NCBI Official Full Name
RNA binding protein fox-1 homolog 3
NCBI Official Synonym Full Names
RNA binding protein, fox-1 homolog (C. elegans) 3
NCBI Official Symbol
RBFOX3
NCBI Official Synonym Symbols
FOX3; NEUN; FOX-3; HRNBP3
NCBI Protein Information
RNA binding protein fox-1 homolog 3; fox-1 homolog C; neuronal nuclei; hexaribonucleotide binding protein 3
UniProt Protein Name
RNA binding protein fox-1 homolog 3
Protein Family
UniProt Gene Name
RBFOX3
UniProt Entry Name
RFOX3_HUMAN
Uniprot Description
NeuN: RNA-binding protein that regulates alternative splicing events.
Protein type: RNA splicing; RNA processing; DNA-binding
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; perikaryon; nucleus
Molecular Function: DNA binding; RNA binding; nucleotide binding
Biological Process: RNA splicing; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome
Research Articles on RBFOX3
Similar Products
Product Notes
The RBFOX3 rbfox3 (Catalog #AAA125033) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NeuN Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's NeuN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF 1:50 - 1:200. Researchers should empirically determine the suitability of the RBFOX3 rbfox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NeuN, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
