Rabbit anti-Human DRAM1 Polyclonal Antibody | anti-DRAM1 antibody

DRAM1, NT (DRAM, DNA Damage-regulated Autophagy Modulator Protein 1, Damage-regulated Autophagy Modulator)

Cat. Num. AAA649575

• Gene Names: DRAM1; DRAM
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DRAM1; Polyclonal Antibody; NT (DRAM; DNA Damage-regulated Autophagy Modulator Protein 1; Damage-regulated Autophagy Modulator); Anti -DRAM1; anti-DRAM1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human DRAM. Species sequence homology: mouse and rat
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-DRAM1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 0.5-1ug/ml; Immunohistochemistry: 2.5ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 3ug/ml
• Immunogen: Synthetic peptide corresponding to 16aa from near the N-terminal of human DRAM (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with DRAM antibody at 3ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Western blot analysis of DRAM in K562 cell lysate with DRAM antibody at (A) 0.5, (B) 1 and (C) 2ug/ml.)

Related Product Information for anti-DRAM1 antibody

Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.

Product Categories/Family for anti-DRAM1 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 110825978
• NCBI GeneID: 55332
• NCBI Accession #: NP_060840.2
• NCBI GenBank Nucleotide #: NM_018370.2
• UniProt Accession #: Q8N682; Q7L3E3; Q9NUN1
• Molecular Weight: 26,253 Da
• NCBI Official Full Name: DNA damage-regulated autophagy modulator protein 1
• NCBI Official Synonym Full Names: DNA-damage regulated autophagy modulator 1
• NCBI Official Symbol: DRAM1
• NCBI Official Synonym Symbols: DRAM
• NCBI Protein Information: DNA damage-regulated autophagy modulator protein 1
• UniProt Protein Name: DNA damage-regulated autophagy modulator protein 1
• Protein Family: DNA damage-regulated autophagy modulator protein
• UniProt Gene Name: DRAM1
• UniProt Synonym Gene Names: DRAM
• UniProt Entry Name: DRAM1_HUMAN

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy. Ref.3 Ref.4

Subcellular location: Lysosome membrane; Multi-pass membrane protein Ref.3.

Induction: By p53/TP53 and p73/TP73. Directly activated by p53/TP53. Significantly down-regulated in tumor cell lines by methylation-dependent transcriptional silencing. Ref.3 Ref.4

Sequence similarities: Belongs to the DRAM/TMEM150 family.

Sequence caution: The sequence BAA92091.1 differs from that shown. Reason: Erroneous initiation.