Western Blot
(Western Blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody MBS9126034 at 1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1uM) at 37°C for 18 hours.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1s.)
Rabbit H3-K9 Antibody | anti-H3K9ac antibody
Acetyl-Histone H3-K9
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:50 - 1:200
ChIP: 1:20 - 1:100
ChIPseq: 1:20 - 1:50
Western Blot
(Western Blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody MBS9126034 at 1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1uM) at 37°C for 18 hours.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1s.)
Western Blot
(Western Blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody MBS9126034 at 1000 dilution. Both NIH/3T3 cells and C6 cells were treated by TSA (1uM) at 37°C for 18 hours.Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) at 10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1s.)
Testing Data
(Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K9ac antibody (MBS9126034) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Testing Data
(Chromatin immunoprecipitation analysis of extracts of 293 cell line, using H3K9ac antibody (MBS9126034) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (MBS9126034) atdilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody (MBS9126034) atdilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (MBS9126034) at dilutionof 1:200 (40x lens))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody (MBS9126034) at dilutionof 1:200 (40x lens))
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-HistoneH3-K9 antibody (MBS9126034) at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-HistoneH3-K9 antibody (MBS9126034) at dilution of 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (MBS9126034) at dilutionof 1:200 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody (MBS9126034) at dilutionof 1:200 (40x lens).)
Immunofluroescence (IF)
(Immunofluorescence analysis of HeLa cellsusing Acetyl-Histone H3-K9 antibody(MBS9126034) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.)
Immunofluroescence (IF)
(Immunofluorescence analysis of HeLa cellsusing Acetyl-Histone H3-K9 antibody(MBS9126034) at dilution of 1:100 (40x lens). HeLa cells were treated by TSA (1 uM) at 37°C for 18 hours (left). Blue: DAPI for nuclear staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3cells using Acetyl-Histone H3-K9 antibody (MBS9126034) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at37°C for 18 hours (left). Blue: DAPI fornuclear staining)
Immunofluorescence (IF)
(Immunofluorescence analysis of NIH/3T3cells using Acetyl-Histone H3-K9 antibody (MBS9126034) at dilution of 1:100 (40x lens). NIH/3T3 cells were treated by TSA (1 uM) at37°C for 18 hours (left). Blue: DAPI fornuclear staining)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (MBS9216034) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit(RM00020)Exposure time: 10s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody (MBS9216034) at 1:1000 dilution.HeLa cells and NIH/3T3 cells and C6 cells were treated by TSA (1 uM) at 37? for 18 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit(RM00020)Exposure time: 10s.)
NCBI and Uniprot Product Information
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Product Notes
The H3K9ac (Catalog #AAA9126034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's H3-K9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), ChIP, ChIPseq. WB: 1:500 - 1:2000 IHC: 1:50 - 1:200 IF: 1:50 - 1:200 IP: 1:50 - 1:200 ChIP: 1:20 - 1:100 ChIPseq: 1:20 - 1:50. Researchers should empirically determine the suitability of the H3K9ac for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "H3-K9, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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