Mouse anti-Human, Mouse Adiponectin Monoclonal Antibody | anti-ADIPOQ antibody

Adiponectin antibody

Cat. Num. AAA200027

• Gene Names: ADIPOQ; ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence
• Purity: By protein-G affinity chromatography
• Synonyms: Adiponectin; Monoclonal Antibody; Adiponectin antibody; ADIPOQ; ACDC; ACRP30; APM1; GBP28; Adiponectin globular domain; Mouse; Adipocyte; C1q and collagen domain-containing protein; 30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; Adipose most abunda; 30 kDa adipocyte complement related protein; ACRP 30; Adipocyte C1q and collagen domain containing protein; Adipocyte complement related 30 kDa protein; Adipocyte complement related protein of 30 kDa; Adipose most abundant gene transcript 1; APM 1; GBP 28; Gelatin binding protein; ; anti-ADIPOQ antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 5H7
• Purity/Purification: By protein-G affinity chromatography
• Form/Format: Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 244

• Applicable Applications for anti-ADIPOQ antibody: ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:1,000 ~ 3,000.; Recommended starting dilution is 1:2,000. Please note that this antibody (5H7) has the specificity against collagen-like domain of adiponectin.
• Antigen Species: Human
• Immunogen: Recombinant human adiponectin (15-244aa) purified from E. coli
• Clone Note: Anti-human adiponectin mAb, clone 5H7, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Acrp30 protein.
• Preparation and Storage: Can be stored at 4 degree C.; For long term storage, aliquot and store at -20 degree C.; Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(The Cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Adiponectin antibody (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. : 293T cell lysate Lane 2. : Adiponectin collagen domain (15-107aa) transfected 293T cell lysate Lane 3. : Adiponectin Full domain (15-244aa) transfected 293T cell lysate Lane 4. : Adiponectin globular domain (108-244aa) transfected 293T cell lysate)

Immunofluorescence (IF)

(ICC/IF analysis of Adiponectin in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Adiponectin antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)

Related Product Information for anti-ADIPOQ antibody

Human Adiponectin, also referred to as AdipoQ, Acrp30, apm-1 or GBP28, is a secreted protein expressed exclusively in differentiated adipocyte (an adipokine family member). Adiponectin contains a modular structure comprising an N-terminal collagenous domain followed by a C-terminal globular domain (gAcrp30). Adiponectin plays a role in various physiological processes such as energy homeostasis and obesity. Plasma levels of adiponectin are reduced in obese humans, and decreased levels are associated with insulin resistance and hyperinsulinemia.

Product Categories/Family for anti-ADIPOQ antibody

Hormones & Cytokines

References

Maeda K. et al., (1996) Biochem Biophys Res Commun. 221:286-9.; Berg AH. et al., (2001) Nat Med; 7:947-53; Berg AH. et al., (2002) Trends Endocrinol Metab. 13: 84-89.; Yamauchi T. et al., (2002) Nat Med. 8:1288-95

NCBI and Uniprot Product Information

• NCBI GI #: 4757760
• NCBI GeneID: 9370
• NCBI Accession #: NP_004788
• NCBI GenBank Nucleotide #: NM_004797.3
• UniProt Accession #: Q15848; Q58EX9
• Molecular Weight: 26,414 Da
• NCBI Official Full Name: adiponectin
• NCBI Official Synonym Full Names: adiponectin, C1Q and collagen domain containing
• NCBI Official Symbol: ADIPOQ
• NCBI Official Synonym Symbols: ACDC; ADPN; APM1; APM-1; GBP28; ACRP30; ADIPQTL1
• NCBI Protein Information: adiponectin
• UniProt Protein Name: Adiponectin
• Protein Family: Adiponectin
• UniProt Gene Name: ADIPOQ
• UniProt Synonym Gene Names: ACDC; ACRP30; APM1; GBP28; ACRP30; apM-1

NCBI Description

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. Mutations in this gene are associated with adiponectin deficiency. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Apr 2010]

Uniprot Description

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.