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Gene Name
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233 results for " adaptive immunity" - showing 1-50
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA700611)
Full Name
Bovine Interleukin 2, IL-2 ELISA Kit
Gene Names
IL2; IL-2; TCGF
Reactivity
Bovine
Pricing
$455/48-Strip-Wells | $640/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,650/5x96-Strip-Wells | $5,045/10x96-Strip-Wells
Flow Cytometry (FC/FACS)
( Flow-cytometry using the anti-Fc gamma receptor III antibody 3G8 (Ab00754) Human peripheral blood leukocytes were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of 3G8 (Ab00754-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)
Fc gamma receptor III, Monoclonal Recombinant Antibody (Cat# AAA488350)
Full Name
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
FC/FACS, SPR, IP, IHC, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA700332)
Full Name
Rat Interleukin 2, IL-2 ELISA Kit
Gene Names
IL2; TCGF
Reactivity
Rat
Pricing
$345/48-Strip-Wells | $475/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,050/5x96-Strip-Wells | $3,915/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA701474)
Full Name
Pig interleukin 2, IL-2 ELISA Kit
Gene Names
IL2; IL-2; TCGF; POIL2
Reactivity
Pig
Pricing
$425/48-Strip-Wells | $595/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,555/5x96-Strip-Wells | $4,865/10x96-Strip-Wells
Western Blot (WB)
(Leptin Receptor (LEPR) Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the LEPR antibody detected the LEPR protein (arrow).)
Leptin Receptor (LEPR), Polyclonal Antibody (Cat# AAA9204850)
Full Name
Leptin Receptor (LEPR) Antibody (N-term)
Gene Names
LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
Reactivity
Human
Applications
WB, EIA, FC/FACS, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
interleukin 2, ELISA Kit (Cat# AAA704522)
Full Name
Mouse Interleukin 2, IL-2 ELISA Kit
Gene Names
Il2; Il-2
Reactivity
Mouse
Pricing
$345/48-Strip-Wells | $475/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,050/5x96-Strip-Wells | $3,915/10x96-Strip-Wells
Western Blot (WB)
CTLA4, Polyclonal Antibody (Cat# AAA540634)
Full Name
CTLA4 Antibody
Gene Names
CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human, Mouse, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488443)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD90/Thy1 using anti- CD90/Thy1 antibody (MBS1750721).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse brain tissue lysates,Lane 2: mouse thymus tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: rat thymus tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD90/Thy1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 26KD. The expected band size for CD90/Thy1 is at 18KD. )
CD90/Thy1, Polyclonal Antibody (Cat# AAA1750721)
Full Name
Anti-CD90/Thy1 Picoband Antibody
Gene Names
Thy1; T25; CD90; Thy-1; Thy1.1; Thy1.2; Thy-1.2
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFAT2 using anti-NFAT2 antibody (MBS1750373).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates,Lane 2: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFAT2 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFAT2 at approximately 101KD. The expected band size for NFAT2 is at 101KD. )
NFAT2, Polyclonal Antibody (Cat# AAA1750373)
Full Name
Anti-NFAT2 Picoband Antibody
Gene Names
NFATC1; NFAT2; NFATc; NF-ATC; NF-ATc1.2
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Fc gamma receptor III, Monoclonal Recombinant Antibody (Cat# AAA488349)
Full Name
Anti-Fc gamma receptor III [3G8]
Reactivity
Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, Squirrel Monkey, Sooty Mangabey, Cotton-topped Tamarin, Marmoset
Applications
FC/FACS, SPR, IP, IHC, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 (MBS488444) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488444, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488444)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
( Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galiximab) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD80, Monoclonal Recombinant Antibody (Cat# AAA488348)
Full Name
Anti-CD80 [IDEC-114 (Galiximab)]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, FC/FACS, WB, EIA, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Figure 4. IHC analysis of CTLA4 using anti-CTLA4 antibody (MBS1750305).
CTLA4 was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CTLA4 Antibody (MBS1750305) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
)
CTLA4, Polyclonal Antibody (Cat# AAA1750305)
Full Name
Anti-CTLA4 Picoband Antibody
Gene Names
Ctla4; Cd152; Ly-56; Ctla-4
Reactivity
Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(HIF1a (Hypoxia-inducible factor 1-alpha) is a subunit of a transcription factor, which functions as a master transcriptional regulator of the adaptive response to hypoxia. The protein chaperone heat shock protein 90 (Hsp90) is a major regulator of different transcription factors, including HIF1a, thus a binding ELISA assay was conducted to detect the interaction of HIF1a and HSP90. Briefly, recombinant human HIF1a were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to HSP90-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-HIF1a pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of of HIF1a and HSP90 was shown in Figure 1, and this effect was in a dose dependent manner.)
Hypoxia Inducible Factor 1 Alpha (HIF1a), Active Protein (Cat# AAA2097345)
Full Name
Active Hypoxia Inducible Factor 1 Alpha (HIF1a)
Gene Names
HIF1A; HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 95%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone PIN 1.1 . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 12 hours at 4 degree C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus. Endosome. Lysosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-CD74 Antibody. (C) Composite.)
CD74, Monoclonal Antibody (Cat# AAA8001876)
Full Name
CD74 Antibody
Gene Names
CD74; II; p33; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IF, IP, FC/FACS
Purity
Protein G Purified
Pricing
$240/0.025 mg | $860/5x0.025 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD166/ALCAM using anti- CD166/ALCAM antibody (MBS1750717).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates,Lane 3: A549 whole Cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- CD166/ALCAM antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD166/ALCAM at approximately 100KD. The expected band size for CD166/ALCAM is at 65KD. )
CD166/ALCAM, Polyclonal Antibody (Cat# AAA1750717)
Full Name
Anti-CD166/ALCAM Picoband Antibody
Gene Names
ALCAM; MEMD; CD166
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of ICOS using anti-ICOS antibody (MBS1750365).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse stomach tissue lysates,Lane 2: mouse spleen tissue lysates,Lane 3: mouse thymus tissue lysates,Lane 4: mouse small intestine tissue lysates,Lane 5: rat thymus tissue lysates,Lane 6: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICOS antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ICOS at approximately 20KD. The expected band size for ICOS is at 22KD. )
ICOS, Polyclonal Antibody (Cat# AAA1750365)
Full Name
Anti-ICOS Picoband antibody
Gene Names
Icos; H4; CCLP; AILIM; CRP-1; Ly115
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
SDS-Page
(SDS-PAGE Analysis Purified Complement 3d Mouse Monoclonal Antibody (C3D/2891). Confirmation of Integrity and Purity of Antibody.)
Complement 3d (C3d), Monoclonal Antibody (Cat# AAA4381315)
Full Name
Complement 3d (C3d) (Acute Humoral Rejection Marker)
Gene Names
C3; ASP; C3a; C3b; AHUS5; ARMD9; CPAMD1; HEL-S-62p
Reactivity
Human
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data
(Figure. The binding activity of IL4 with IL2Rg.The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IL4 and recombinant rat IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was shown in Figure 1, and this effect was in a dose dependent manner.)
Interleukin 4 (IL4), Active Protein (Cat# AAA2105071)
Full Name
Active Interleukin 4 (IL4)
Gene Names
Il4; Il4e12
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$240/0.01 mg | $515/0.05 mg | $795/0.1 mg | $985/0.2 mg | $1,920/0.5 mg | $2,845/1 mg | $6,960/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of CD40L using anti-CD40L antibody (MBS1750574).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD40L antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD40L at approximately 36KD. The expected band size for CD40L is at 29KD. )
CD40L, Polyclonal Antibody (Cat# AAA1750574)
Full Name
Anti-CD40L Picoband antibody
Gene Names
Cd40lg; IGM; IMD3; Ly62; TRAP; gp39; CD154; Cd40l; HIGM1; Ly-62; T-BAM; CD40-L; Tnfsf5; Tnlg8b
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD44 antibody Hermes-3 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Hermes-3 (MBS488447, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD44, Monoclonal Recombinant Antibody (Cat# AAA488447)
Full Name
Anti-CD44 [Hermes -3]
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human
Applications
BL, WB, IHC, IF, FC/FACS, ICC, EIA
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488454)
Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Thrombopoietin using anti-Thrombopoietin antibody (MBS1750819).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Thrombopoietin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Thrombopoietin at approximately 38KD. The expected band size for Thrombopoietin is at 38KD. )
Thrombopoietin, Polyclonal Antibody (Cat# AAA1750819)
Full Name
Anti-Thrombopoietin Picoband Antibody
Gene Names
Thpo; Ml; Tpo; Mgdf; Mpllg
Reactivity
Mouse, Rat
No cross reactivity with other proteins
No cross reactivity with other proteins
Applications
EIA, IHC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Activity Data
(The interleukin 4 (IL4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL4, Th2 cells subsequently produce additional IL4 in a positive feedback loop. IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL12. Besides, Interleukin 2 Receptor Gamma (IL2Rg) has been identified as an interactor of IL4, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IL4 and recombinant mouse IL2Rg. Briefly, IL4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100muL were then transferred to IL2Rg-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL4 and IL2Rg was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of IL4 with IL2Rg.)
Interleukin 4 (IL4), Active Protein (Cat# AAA2105070)
Full Name
Active Interleukin 4 (IL4)
Gene Names
Il4; Il-4; BSF-1
Applications
Cell Culture, Activity Assays
Purity
> 97%
Pricing
$195/0.01 mg | $400/0.05 mg | $615/0.1 mg | $755/0.2 mg | $1,460/0.5 mg | $2,175/1 mg | $5,305/5 mg
Western Blot (WB)
(Figure 1. Western blot analysis of NFATC4 using anti-NFATC4 antibody (MBS1750778).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat testis tissue lysates,Lane 2: mouse testis tissue lysates,Lane 3: human Hela whole cell lysates,Lane 4: human placenta tissue lysates,Lane 5: human A549 whole cell lysates,Lane 6: human SK-OV-3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NFATC4 antigen affinity purified polyclonal antibody at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFATC4 at approximately 95120KD. The expected band size for NFATC4 is at 95KD. )
NFATC4, Polyclonal Antibody (Cat# AAA1750778)
Full Name
Anti-NFATC4 Picoband Antibody
Gene Names
NFATC4; NFAT3; NF-AT3; NF-ATC4
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
EIA, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD81 antibody 1D6 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 (MBS488457) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488457, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
CD81, Monoclonal Recombinant Antibody (Cat# AAA488457)
Full Name
Anti-CD81 [1D6]
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Chimpanzee, Goat, Sheep
Applications
EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10 Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 (MBS488455) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488455, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
CD27, Monoclonal Recombinant Antibody (Cat# AAA488455)
Full Name
Anti-CD27 [LG.3A10]
Gene Names
Cd27; S152; Tp55; Tnfrsf7
Reactivity
Mouse
Applications
IP, IF, FC/FACS
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD62L antibody DREG-56 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of DREG-56 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD62L, Monoclonal Recombinant Antibody (Cat# AAA488437)
Full Name
Anti-CD62L [DREG-56]
Gene Names
SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
Reactivity
Human, Chimpanzee, Bovine
Applications
IP, FC/FACS, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
CD80, Monoclonal Recombinant Antibody (Cat# AAA488347)
Full Name
Anti-CD80 [IDEC-114 (Galiximab)]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, FC/FACS, WB, EIA, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Jurkat cells with anti-CD81 antibody 1D6 Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of 1D6 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
CD81, Monoclonal Recombinant Antibody (Cat# AAA488456)
Full Name
Anti-CD81 [1D6]
Gene Names
CD81; S5.7; CVID6; TAPA1; TSPAN28
Reactivity
Human, Chimpanzee, Goat, Sheep
Applications
EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD44 antibody Hermes-1 Human activated T lymphocytes were stained with an isotype control or the rabbit-chimeric version of Hermes-1 (MBS488445, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD44, Monoclonal Recombinant Antibody (Cat# AAA488445)
Full Name
Anti-CD44 [Hermes-1]
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine
Applications
BL, EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488442)
Full Name
Anti-Integrin beta-7 [FIB504]
Gene Names
Itgb7; Ly69
Reactivity
Human, Mouse
Applications
IF, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB504 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB504 (MBS488441) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488441, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488441)
Full Name
Anti-Integrin beta-7 [FIB504]
Gene Names
Itgb7; Ly69
Reactivity
Human, Mouse
Applications
IF, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD62L antibody DREG-56 Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of DREG-56 (MBS488438, panel B) at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD62L, Monoclonal Recombinant Antibody (Cat# AAA488438)
Full Name
Anti-CD62L [DREG-56]
Gene Names
SELL; TQ1; LAM1; LEU8; LNHR; LSEL; CD62L; LYAM1; PLNHR; LECAM1
Reactivity
Human, Chimpanzee, Bovine
Applications
IP, FC/FACS, WB, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Flow Cytometry (FC/FACS)
(Flow-cytometry using anti-CD44 antibody Hermes-1 Human activated T lymphocytes were stained with an isotype control or the rabbit-chimeric version of Hermes-1 at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD44, Monoclonal Recombinant Antibody (Cat# AAA488446)
Full Name
Anti-CD44 [Hermes-1]
Gene Names
CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III
Reactivity
Human, Mouse, Rat, Goat, Primate, Rabbit, Sheep, Bovine
Applications
BL, EIA, FC/FACS, ICC, IF, IHC, IP, WB
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
MOMA2, Monoclonal Antibody (Cat# AAA530837)
Full Name
MOMA2 antibody (Mouse)
Reactivity
Murine
Applications
FC/FACS, IHC
Pricing
$710/0.2 mg | $3,150/5x0.2 mg
Immunohistochemistry
(Immunohistochemical staining using Follicular Dendritic Cells antibodyRat spleen frozen tissue section)
Follicular Dendritic Cells, Monoclonal Antibody (Cat# AAA532042)
Full Name
Follicular Dendritic Cells antibody
Gene Names
FDCSP; C4orf7; FDC-SP
Reactivity
Rat
Applications
IHC
Immunohistochemistry (IHC)
CD80, Polyclonal Antibody (Cat# AAA540015)
Full Name
CD80 Antibody
Gene Names
Cd80; B7-1
Reactivity
Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Macrophage, Polyclonal Antibody (Cat# AAA534052)
Full Name
Rat Macrophage antibody
Reactivity
Rat
Applications
User optimized
Purity
Rat Macrophage antibody was purified by Salt fractionation, ion exchange chromatography and gel filtration.
Pricing
$510/10 mg | $2,275/5x10 mg
Immunohistochemistry (IHC)
CD2, Polyclonal Antibody (Cat# AAA540769)
Full Name
CD2 Antibody
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human, Monkey
Predicted:Mouse, Rat
Predicted:Mouse, Rat
Applications
EIA, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry (IHC)
CD2, Polyclonal Antibody (Cat# AAA540898)
Full Name
CD2 Antibody
Gene Names
CD2; T11; SRBC; LFA-2
Reactivity
Human, Monkey
Predicted: Mouse, Rat
Predicted: Mouse, Rat
Applications
EIA, IP, IHC, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunohistochemistry
(Immunohistochemical staining using Mouse Pan Macrophages antibodyMouse spleen frozen tissue section)
Pan Macrophages, Monoclonal Antibody (Cat# AAA530836)
Full Name
Mouse Pan Macrophages antibody
Reactivity
Murine
Applications
IHC
Pricing
$1,075/0.2 mg | $4,770/5x0.2 mg
Typical Testing Data/Standard Curve (for reference only)
CD4 molecule, ELISA Kit (Cat# AAA702845)
Full Name
Human cluster of differentiation 4, CD4 ELISA Kit
Gene Names
CD4; CD4mut
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
MHC class I polypeptide-related sequence A, ELISA Kit (Cat# AAA703262)
Full Name
Human major histocompatibility complex class I chain-related gene A, MICA ELISA Kit
Gene Names
MICA; MIC-A; PERB11.1
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells
tumor necrosis factor receptor superfamily, member 13B, ELISA Kit (Cat# AAA931344)
Full Name
Human Tumor necrosis factor receptor superfamily member 13B, TNFRSF13B ELISA Kit
Gene Names
TNFRSF13B; CVID; RYZN; TACI; CD267; CVID2; TNFRSF14B
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells
Western Blot (WB)
Prlz / TPD52, Polyclonal Antibody (Cat# AAA540126)
Full Name
PrLZ / TPD52 Antibody Affinity Purified
Gene Names
TPD52; D52; N8L; PC-1; PrLZ; hD52
Reactivity
Human
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg
Immunoassay (IA)
(The calibration curve of a sandwich assay for measurement of C3a(desArg) using MBS838793 as the capture antibody and MBS838118 as the biotinylated detection antibody.)
C3a desArg, Monoclonal Antibody (Cat# AAA838793)
Full Name
C3a desArg antibody
Reactivity
Human
Applications
EIA, WB
Purity
C3a desArg antibody was purified from cell culture supernatant by protein A/G chromatography
Pricing
$1,765/0.4 mL | $7,935/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
cytotoxic T-lymphocyte-associated protein 4, ELISA Kit (Cat# AAA700192)
Full Name
Human cytotoxic T lymphocyte associated antigen 4, CTLA-4 ELISA Kit
Gene Names
CTLA4; CD; GSE; GRD4; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells
Western Blot (WB)
(Western blot analysis of lysates from Raji cellline, untreated or treated with TPA, 200nM, 30min, using (MBS9202170)(upper) or Tubulin (lower).)
Bi-Phospho-Syk (Y525/526), Polyclonal Antibody (Cat# AAA9202170)
Full Name
Bi-Phospho-Syk (Y525/526) Antibody
Gene Names
SYK; p72-Syk
Reactivity
Human (Predicted Reactivity: Mouse, Rat, Pig)
Applications
WB, EIA, IHC-P
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
