anti-Human Mesothelin Monoclonal Antibody | anti-MSLN antibody

Mesothelin

Cat. Num. AAA600014

• Gene Names: MSLN; MPF; SMRP
• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Mesothelin; Monoclonal Antibody; Anti -Mesothelin; anti-MSLN antibody

• Host: Host: Rat; Source: Human
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2A
• Clone Number: 8B35
• Specificity: Recognizes human mesothelin.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
• Concentration: ~0.5mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-MSLN antibody: ELISA (EL/EIA), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry and Direct ELISA.; Dilution: Flow Cytometry: 2.5ug/10e6 cells. Validated using HeLa human cervical carcinoma cells.
• Immunogen: Recombinant protein corresponding to aa296-580 from human mesothelin, expressed in NSO cells (ABW03459).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Detection of Mesothelin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with M300-01C or isotype control antibody followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody.)

Related Product Information for anti-MSLN antibody

Mesothelin is a 40kD glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinoma. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1kD) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.

Product Categories/Family for anti-MSLN antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 293651530
• NCBI GeneID: 10232
• NCBI Accession #: AAH09272
• NCBI GenBank Nucleotide #: NM_001177355.1
• NCBI Official Full Name: mesothelin isoform 1 preproprotein
• NCBI Official Synonym Full Names: mesothelin
• NCBI Official Symbol: MSLN
• NCBI Official Synonym Symbols: MPF; SMRP
• NCBI Protein Information: mesothelin; CAK1 antigen; megakaryocyte potentiating factor; soluble MPF mesothelin related protein; pre-pro-megakaryocyte-potentiating factor
• UniProt Protein Name: Mesothelin
• Protein Family: Mesothelin
• UniProt Gene Name: MSLN
• UniProt Synonym Gene Names: MPF; MPF
• UniProt Entry Name: MSLN_HUMAN