Rabbit Bi-Phospho-Syk (Y525/526) Polyclonal Antibody | anti-SYK antibody

Bi-Phospho-Syk (Y525/526) Antibody

Cat. Num. AAA9202170

• Gene Names: SYK; p72-Syk
• Reactivity: Human (Predicted Reactivity: Mouse, Rat, Pig)
• Applications: Western Blot, ELISA, Immunohistochemistry
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: Bi-Phospho-Syk (Y525/526); Polyclonal Antibody; Bi-Phospho-Syk (Y525/526) Antibody; Tyrosine-protein kinase SYK; Spleen tyrosine kinase; p72-Syk; SYK; anti-SYK antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Mouse, Rat, Pig)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Syk Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Y525/526 of human Syk.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Length: 612

• Applicable Applications for anti-SYK antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P)
• Application Notes: WB~~1:250; IHC-P~~1:50~100
• Function: Non-receptor tyrosine kinase which mediatessignal transduction downstream of a varietyof transmembrane receptors includingclassical immunoreceptors like the B-cellreceptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclastmaturation, platelet activation and vasculardevelopment. Assembles into signalingcomplexes with activated receptors at theplasma membrane via interaction between itsSH2 domains and the receptor tyrosine-phosphorylated ITAM domains. Theassociation with the receptor can also beindirect and mediated by adapter proteins containing ITAM or partial hemITAM domains.The phosphorylation of the ITAM domains isgenerally mediated by SRC subfamily kinases upon engagement of the receptor. Morerarely signal transduction via SYK could be ITAM-independent. Direct down stream effectors phosphorylated by SYK include VAV1, PLCG1, PI-3-kinase, LCP2 and BLNK. Initially identified as essential in B-cellreceptor (BCR) signaling, it is necessary forthe maturation of B-cells most probably at the pro-B to pre-B transition. Activated upon BCR engagement, it phosphorylates and activates BLNK an adapter linking the activated BCR to downstream signaling adapters and effectors. It also phosphorylates and activates PLCG1 and the PKC signaling pathway. It also phosphorylates BTK and regulates its activity in B-cell antigenreceptor (BCR)-coupled signaling. In addition to its function downstream of BCR plays alsoa role in T- cell receptor signaling. Plays alsoa crucial role in the innate immune response to fungal, bacterial and viral pathogens. It isfor instance activated by the membranelectin CLEC7A. Upon stimulation by fungalproteins, CLEC7A together with SYK activates immune cells inducing the production of ROS. Also activates the inflammasome and NF-kappa-B-mediated transcription of chemokines and cytokines in presence ofpathogens. Regulates neutrophildegranulation and phagocytosis throughactivation of the MAPK signaling cascade (Bysimilarity). Required for the stimulation ofneutrophil phagocytosis by IL15 (PubMed:15123770). Alsomediates the activation of dendritic cells bycell necrosis stimuli. Also involved in mastcells activation. Involved in interleukin-3/IL3-mediated signaling pathway in basophils (Bysimilarity). Also functions downstream ofreceptors mediating cell adhesion. Relays forinstance, integrin-mediated neutrophils andmacrophages activation and P-selectinreceptor/SELPG-mediated recruitment ofleukocytes to inflammatory loci. Plays also arole in non-immune processes. It is for instance involved in vascular development where it may regulate blood and lymphaticvascular separation. It is also required forosteoclast development and function.Functions in the activation of platelets bycollagen, mediating PLCG2 phosphorylation and activation. May be coupled to thecollagen receptor by the ITAMdomain-containing FCER1G. Also activated bythe membrane lectin CLEC1B that is required for activation of platelets byPDPN/podoplanin. Involved in platelet adhesion being activated by ITGB3 engagedby fibrinogen. Together with CEACAM20,enhances production of the cytokineCXCL8/IL-8 via the NFKB pathway and maythus have a role in the intestinal immuneresponse (By similarity).
• Cellular Location: Cell membrane. Cytoplasm, cytosol
• Tissue Location: Widely expressed in hematopoietic cells (atprotein level) (PubMed:8163536). Expressedin neutrophils (at protein level)(PubMed:15123770). Within the B-cellcompartment, expressed from pro- and pre-Bcells to plasma cells (PubMed:8163536).
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from Raji cellline, untreated or treated with TPA, 200nM, 30min, using (MBS9202170)(upper) or Tubulin (lower).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-SYK antibody

Syk is a positive effector of BCR-stimulated responses. This protein couples the B-cell antigen receptor (BCR) to the mobilization of calcium ion either through a phosphoinositide 3-kinase-dependent pathway, when not phosphorylated on tyrosines of the linker region, or through a phospholipase C-gamma-dependent pathway, when phosphorylated on Tyr-342 and Tyr-346. Thus the differential phosphorylation of Syk can determine the pathway by which BCR is coupled to the regulation of intracellular calcium ion.

Product Categories/Family for anti-SYK antibody

Cardiovascular; Immunology; Signal Transduction; Phospho Antibodies

References

Steinberg, M., et al., Blood 104(3):760-767 (2004). Wang, S., et al., World J. Gastroenterol. 10(12):1815-1818 (2004). Hayes, M.J., et al., Mol. Immunol. 41(4):457-468 (2004). Arndt, P.G., et al., J. Biol. Chem. 279(12):10883-10891 (2004). Ding, Y.B., et al., Zhonghua Yi Xue Za Zhi 84(4):290-293 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 205277435
• NCBI GeneID: 6850
• NCBI Accession #: NP_001128524.1; NP_001167638.1; NP_001167639.1; NP_003168.2
• NCBI GenBank Nucleotide #: NM_001135052.3
• UniProt Accession #: P43405
• NCBI Official Full Name: tyrosine-protein kinase SYK isoform Syk(S)
• NCBI Official Synonym Full Names: spleen tyrosine kinase
• NCBI Official Symbol: SYK
• NCBI Official Synonym Symbols: p72-Syk
• NCBI Protein Information: tyrosine-protein kinase SYK
• UniProt Protein Name: Tyrosine-protein kinase SYK
• Protein Family: Tyrosine-protein kinase
• UniProt Gene Name: SYK
• UniProt Entry Name: KSYK_HUMAN

NCBI Description

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]

Uniprot Description

Syk: a cytoplasmic tyrosine kinase of the SYK family containing two SH2 domains. Plays a central role in the B cell receptor (BCR) response. An upstream activator of the PI3K, PLCgamma2, and Rac/cdc42 pathways in the BCR response. Required for the sequential events of Fc gamma IIa receptor-mediated phagocytosis. Expression highest in murine spleen, heart, mammary gland and thymus. Two splice variant isoforms have been described.

Protein type: Protein kinase, tyrosine (non-receptor); Protein kinase, TK; Kinase, protein; EC 2.7.10.2; TK group; Syk family

Chromosomal Location of Human Ortholog: 9q22

Cellular Component: T cell receptor complex; extrinsic to internal side of plasma membrane; protein complex; cytoplasm; plasma membrane; cytosol; nucleus; B cell receptor complex

Molecular Function: integrin binding; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: viral reproduction; transcription factor import into nucleus; positive regulation of interleukin-3 biosynthetic process; positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; positive regulation of calcium-mediated signaling; regulation of phagocytosis; protein amino acid phosphorylation; activation of JNK activity; B cell receptor signaling pathway; leukocyte adhesion; regulation of transcription factor activity; positive regulation of bone resorption; beta selection; positive regulation of gamma-delta T cell differentiation; angiogenesis; inflammatory response; integrin-mediated signaling pathway; platelet activation; neutrophil chemotaxis; adaptive immune response; regulation of superoxide release; positive regulation of cell adhesion mediated by integrin; positive regulation of mast cell degranulation; regulation of neutrophil degranulation; leukotriene biosynthetic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; organ morphogenesis; neutrophil activation during immune response; serotonin secretion by platelet; leukocyte activation during immune response; macrophage activation during immune response; defense response to bacterium; blood vessel morphogenesis; positive regulation of B cell differentiation; innate immune response; positive regulation of alpha-beta T cell proliferation; lymph vessel development; blood coagulation; positive regulation of alpha-beta T cell differentiation; transmembrane receptor protein tyrosine kinase signaling pathway; positive regulation of cytokine secretion