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565 results for " CD28 Human" - showing 1-50

Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin (MBS210509))

CD28, Monoclonal Antibody (Cat# AAA211263)

Full Name
RAT ANTI HUMAN CD28:Low Endotoxin
Gene Names
CD28; Tp44
Applications
FC/FACS, FN
Pricing
$745/0.5 mg | $3,200/5x0.5 mg

transmembrane and immunoglobulin domain containing 2, ELISA Kit (Cat# AAA9317683)

Full Name
Human Transmembrane and immunoglobulin domain-containing protein 2, TMIGD2 ELISA Kit
Gene Names
TMIGD2; IGPR1; IGPR-1
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)

CD80 molecule, ELISA Kit (Cat# AAA705535)

Full Name
Human Soluble Cluster of Differentiation 80, sCD80 ELISA Kit
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Pricing
$470/48-Strip-Wells | $665/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,750/5x96-Strip-Wells | $5,230/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)

Cytotoxic T-lymphocyte protein 4, ELISA Kit (Cat# AAA765277)

Full Name
Human Cytotoxic T-lymphocyte protein 4 ELISA Kit
Gene Names
Ctla4; Cd152; CTLA-4; sCTLA4
Reactivity
Human
Pricing
$330/48-Strip-Wells | $445/96-Strip-Wells | $1,995/5x96-Strip-Wells | $3,805/10x96-Strip-Wells
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Esophageal Tumor stained with CD86 Monoclonal Antibody (BU63).)

CD86, Monoclonal Antibody (Cat# AAA438121)

Full Name
CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, WB, IP, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western Blot Analysis of Daudi Cell Lysate using CD86 Monoclonal Antibody (C86/1146).)

CD86, Monoclonal Antibody (Cat# AAA438848)

Full Name
CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Typical Testing Data/Standard Curve (for reference only)

CD86 molecule, ELISA Kit (Cat# AAA702416)

Full Name
Human soluble Cluster of differentiation 86, sCD86 ELISA Kit
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human
Pricing
$385/48-Strip-Wells | $545/96-Strip-Wells | $265/24-Strip-Wells (LIMIT 1) | $2,360/5x96-Strip-Wells | $4,490/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)

CD28, ELISA Kit (Cat# AAA2509748)

Full Name
Human CD28 (Cluster of Differentiation 28) ELISA Kit
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Immunofluorescence (IF) (Immunofluorescent staining of Jurkat cells using CD28 Mouse Monoclonal Antibody (CB28) followed by goat anti-Mouse IgG conjugated to CF488 (green). Red Dot is used to label nuclei (red).)

CD28, Monoclonal Antibody (Cat# AAA438325)

Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human
Applications
FC/FACS, IF, FS
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

PD-L1 / PDCD1LG1 / CD274 / B7-H1, Antibody (Cat# AAA4380524)

Full Name
PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Mouse Monoclonal Antibody [Clone PDL1/2746]
Reactivity
Human, Mouse
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin (MBS210509))

CD28, Monoclonal Antibody (Cat# AAA216528)

Full Name
RAT ANTI HUMAN CD28
Gene Names
CD28; Tp44
Applications
FC/FACS
Purity
Purified
Purified IgG - liquid
Pricing
$195/0.025 mg | $730/5x0.025 mg

CD28, Monoclonal Antibody (Cat# AAA438303)

Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human.
Applications
EIA, FC/FACS, IF, IP, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western Blot Analysis of HeLa, Raji, MDA231, MCF-7, NIH3T3, Panc-1, cell lysates using PD-L2 Mouse Monoclonal Antibody (PDL1/2744).)

PD-L1/PDCD1LG1/CD274/B7-H1, Monoclonal Antibody (Cat# AAA4380958)

Full Name
PD-L1/PDCD1LG1/CD274/B7-H1 (Cancer Immunotherapy Target)
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; hPD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse
Applications
EIA, WB
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data (Formalin-fixed, paraffin-embedded human esophageal tumor stained with CD86 MAb (BU63).)

CD86, Monoclonal Antibody (Cat# AAA438204)

Full Name
CD86
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat.
Applications
FC/FACS
Purity
Purified Ab conjugated to FITC
Pricing
$390/0.5 mL | $1,710/5x0.5 mL
Flow Cytometry (FC/FACS) (Human peripheral blood lymphocytes are stained with Anti-Human CD28 Monoclonal Antibody(PerCP/Cyanine5.5 Conjugated)(filled gray histogram). Unstained lymphocytes (empty black histogram) are used as control.)

CD28, Monoclonal Antibody (Cat# AAA2559441)

Full Name
Anti-Human CD28 Monoclonal Antibody (PerCP/Cyanine5.5 Conjugated) [CD28.2]
Gene Names
CD28; Tp44
Reactivity
Human
Applications
FC/FACS
Pricing
$190/100 Tests | $245/200 Tests | $165/50 Tests | $140/20 Tests | $1,100/5x200 Tests
Testing Data (Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

CD18, Monoclonal Antibody (Cat# AAA213057)

Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Reactivity
Dog, Guinea Pig
Applications
FC/FACS, IP
Pricing
$475/0.2 mg | $1,995/5x0.2 mg
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Lymph Node stained with CD80 Mouse Monoclonal Antibody (C80/2723).)

CD80, Monoclonal Antibody (Cat# AAA4380739)

Full Name
CD80 (B7-1)
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human. Others not known.
Applications
EIA
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Melanoma stained with CD86 Monoclonal Antibody (SPM600).)

CD86, Monoclonal Antibody (Cat# AAA438773)

Full Name
CD86 (Dendritic Cells Maturation Marker) Mouse Monoclonal Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data (FCM staining of human PBMCs using CD86 MAb (BU63).)

CD86, Monoclonal Antibody (Cat# AAA438023)

Full Name
CD86
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat.
Applications
FC/FACS, IF
Purity
Purified Ab conjugated to PE
Pricing
$390/0.5 mL | $1,710/5x0.5 mL

CD80 (B7-1), Active Protein (Cat# AAA553137)

Full Name
Human CD80 Recombinant (B7-1)
Purity
>90%, as determined by SDS-PAGE and HPLC
Pricing
$245/0.05 mg | $640/0.2 mg | $2,350/1 mg

CD28, Monoclonal Antibody (Cat# AAA438728)

Full Name
CD28 Mouse Monoclonal Antibody
Gene Names
CD28; Tp44
Reactivity
Human, Pig, Cow, Sheep, Mouse
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of extracts of THP-1 cells, using CD86 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

CD86, Polyclonal Antibody (Cat# AAA126270)

Full Name
CD86 Polyclonal Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse
Applications
WB, IHC, ICC, IP
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)

CTLA4, Polyclonal Antibody (Cat# AAA540634)

Full Name
CTLA4 Antibody
Gene Names
CTLA4; CD; GSE; GRD4; ALPS5; CD152; CTLA-4; IDDM12; CELIAC3
Reactivity
Human, Mouse, Rat
Applications
EIA, ICC, IF, IHC, IP, WB
Pricing
$445/0.1 mg | $1,855/5x0.1 mg

CD28, Monoclonal Antibody (Cat# AAA438180)

Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human.
Applications
FC/FACS
Purity
Purified Ab conjugated to PE
Pricing
$390/0.5 mL | $1,710/5x0.5 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Cervical Squamous Cell Carcinoma stained with CD44v4 Mouse Recombinant Monoclonal Antibody (rCD44v4/1219).)

CD28, Monoclonal Antibody (Cat# AAA4380332)

Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human. Others not known.
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded Human Colon stained with CD86 Rabbit Recombinant Monoclonal Antibody (C86/2160R).)

CD86, Monoclonal Antibody (Cat# AAA4380337)

Full Name
CD86 (Dendritic Cells Maturation Marker)
Reactivity
Human, Mouse, Rat.
Applications
FC, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Testing Data (Figure A. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD61 and Alexa Fluor 647 conjugated Mouse anti Human CD29 . All experiments performed on red cell lysed human blood gated on live platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

CD29, Monoclonal Antibody (Cat# AAA225642)

Full Name
Mouse Anti Human CD29: RPE
Gene Names
ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
Reactivity
Human, Mink
Does not react with: Rat, Mouse
Applications
FC/FACS
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Pricing
$500/100 Tests | $2,090/5x100 Tests
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using CD86 Antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

CD86, Polyclonal Antibody (Cat# AAA127350)

Full Name
CD86
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human
Applications
WB, IHC
Purity
Affinity purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Colon stained with CD86 Mouse Recombinant Monoclonal Antibody (rC86/1146).)

CD86, Monoclonal Antibody (Cat# AAA4380336)

Full Name
CD86 (Dendritic Cells Maturation Marker)
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human, Mouse, Rat. Others not known.
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

CD28, Monoclonal Antibody (Cat# AAA438024)

Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human.
Applications
EIA, FC/FACS, IF, IP, IH
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB) (Western blot analysis of extracts from mouse spleen, using CD28 Antibody.The lane on the right is treated with the antigen-specific peptide.)

CD28, Polyclonal Antibody (Cat# AAA9600003)

Full Name
CD28 Antibody
Gene Names
CD28; Tp44
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

CD18, Monoclonal Antibody (Cat# AAA213058)

Full Name
RAT ANTI HUMAN CD18
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, IP
Pricing
$340/0.1 mg | $1,375/5x0.1 mg
Testing Data (Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

CD18, Monoclonal Antibody (Cat# AAA213845)

Full Name
RAT ANTI HUMAN CD18:RPE
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS
Pricing
$500/100 Tests | $2,090/5x100 Tests
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Cervix Carcinoma stained with CD44v6 Mouse Monoclonal Antibody (CD44V6/2496).)

CD80, Monoclonal Antibody (Cat# AAA4380335)

Full Name
CD80 (B7-1)
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human. Others not known.
Applications
EIA, IF, FC/FACS
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Activity Data (Cluster Of Differentiation 276 (CD276) also known as B7-H3 in human, is a member of the B7/CD28 superfamily of costimulatory molecules serving as an accessory modulator of T-cell response. B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share about 20-40% amino acid (aa) sequence identity. CD276 may play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. It has also been found to enhance the induction of primary cytotoxic T lymphocytes and stimulate IFN-gamma production. Besides, Sialic acid-binding Ig-like lectin 12 (SIGLEC12) has been identified as an interactor of CD276, thus a binding ELISA assay was conducted to detect the interaction of recombinant human CD276 and recombinant human SIGLEC12. Briefly, CD276 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to SIGLEC12-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CD276 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of CD276 and SIGLEC12 was shown in Figure 1, and this effect was in a dose dependent manner.)

Cluster Of Differentiation 276 (CD276), Active Protein (Cat# AAA2097281)

Full Name
Active Cluster Of Differentiation 276 (CD276)
Gene Names
CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3
Reactivity
Human
Applications
Cell culture, Activity Assays
Purity
> 97%
Pricing
$250/0.01 mg | $540/0.05 mg | $840/0.1 mg | $1,045/0.2 mg | $2,040/0.5 mg | $3,020/1 mg | $7,385/5 mg
Flow Cytometry (FC/FACS) ( Flow-cytometry using anti-GITR antibody YGITR765 Mouse activated T cells were stained with an isotype control or the rabbit-chimeric version of YGITR765 at a concentration of 1 ug/ml for 30 mins at RT without (Blue) and with (Grey) stimulation of the lymphocytes with immobilized anti-CD3 and anti-CD28 antibodies. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)

GITR, Monoclonal Recombinant Antibody (Cat# AAA488333)

Full Name
Anti-GITR [YGITR765]
Gene Names
TNFRSF18; GITR
Reactivity
Mouse
Applications
FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC) (MBS9606990 at 1/100 staining Human lymph tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

CD86, Polyclonal Antibody (Cat# AAA9606990)

Full Name
CD86 Antibody
Gene Names
CD86; B70; B7-2; B7.2; LAB72; CD28LG2
Reactivity
Human
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
SDS-Page (Recombinant Human TMIGD2/IGPR1 Protein was determined by SDS-PAGE under reducing conditions with Coomassie Blue.)

TMIGD2/IGPR1, Recombinant Protein (Cat# AAA9140239)

Full Name
Recombinant Human TMIGD2/IGPR1 Protein
Gene Names
TMIGD2; CD28H; IGPR1; IGPR-1
Purity
>95% by SDS-PAGE.
Pricing
$215/0.01 mg | $435/0.05 mg | $1,420/0.5 mg | $1,990/1 mg | $8,900/5x1 mg
Western Blot (WB) (Western blot detection of GRB2 in K562, Raji, Jurkat, Ramos, C6, 3T3 and Hela cell lysates using GRB2 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa.)

GRB2, Monoclonal Antibody (Cat# AAA475234)

Full Name
Anti-GRB2 Mouse mAb
Gene Names
GRB2; ASH; Grb3-3; MST084; NCKAP2; MSTP084; EGFRBP-GRB2
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Western Blot (WB) (Western blot analysis of CD80 using K562 whole lysates.)

CD80, Polyclonal Antibody (Cat# AAA9608264)

Full Name
CD80 Antibody
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data (Published customer image: R-phycoerythrin conjugated Rat anti Mouse CD18 antibody, clone YFC118.3 used for flow cytometryImage caption:The levels of CD11b and CD18 fall dramatically following incubation with Leishmania under serum dependent (SD) culture conditions. Peritoneal macrophages were selected based on forward- versus side-scatter parameters as described in Materials and Methods and Figure 1 using flow cytometry. A) Isotype control with macrophages showing delineation of the M1 marker based on the negative population. B and C represent expression of CD18 (B) or CD11b (C) before incubation with Leishmania. D and E represent expression of CD18 (D) or CD11b (E) after association with Leishmania in a SD assay. In all cases the percent positive cells within the M1 marker are identified. This Figure is a representative diagram of 4 experiments with similar findings.From: Gonçalves R, Vieira ER, Melo MN, Gollob KJ, Mosser DM, Tafuri WL. A sensitive flow cytometric methodology for studying the binding of L. chagasi to canine peritoneal macrophages. BMC Infect Dis. 2005 May 24;5:39.)

CD18, Monoclonal Antibody (Cat# AAA219819)

Full Name
RAT ANTI HUMAN CD18:Low Endotoxin
Gene Names
ITGB2; LAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Applications
FC/FACS, FN, IP
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
SDS-Page (SDS-PAGE Analysis Purified CD28 Mouse Monoclonal Antibody (CB28). Confirmation of Integrity and Purity of Antibody.)

CD28, Monoclonal Antibody (Cat# AAA4381417)

Full Name
CD28
Gene Names
CD28; Tp44
Reactivity
Human
Applications
FC/FACS, IF
Purity
Purified Ab conjugated to CF488
Pricing
$390/0.5 mL | $1,710/5x0.5 mL
Immunohistochemistry (IHC) (MBS9600688 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

LCP1, Polyclonal Antibody (Cat# AAA9600688)

Full Name
Phospho-LCP1 (Tyr28) Antibody
Gene Names
LCP1; LPL; CP64; PLS2; LC64P; HEL-S-37; L-PLASTIN
Reactivity
Human, Mouse
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB) (Western blot analysis of Phospho-CD28 (Tyr218) using Jurkat whole cell lysates)

CD28, Polyclonal Antibody (Cat# AAA9609052)

Full Name
Phospho-CD28 (Tyr218) Antibody
Gene Names
CD28; Tp44
Reactivity
Human, Mouse, Rat
Applications
WB, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunofluorescence (IF) ( Immunofluorescence staining of fixed Daudi cells with anti-CD80 antibody IDEC-114 (Galiximab) Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of IDEC-114 (Galiximab) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)

CD80, Monoclonal Recombinant Antibody (Cat# AAA488348)

Full Name
Anti-CD80 [IDEC-114 (Galiximab)]
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
BL, FC/FACS, WB, EIA, IP, IHC
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB) (Western blot analysis of Jurkat whole cell lysates, using ICOS Antibody. The lane on the left is treated with the antigen-specific peptide.)

ICOS, Polyclonal Antibody (Cat# AAA9607287)

Full Name
ICOS Antibody
Gene Names
ICOS; AILIM; CD278; CVID1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC) (Formalin-fixed, paraffin-embedded human Tonsil stained with CD80 Mouse Monoclonal Antibody (C80/2776).)

CD80, Monoclonal Antibody (Cat# AAA4380740)

Full Name
CD80 (B7-1)
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human. Others not known.
Applications
EIA, Formalin-Fixed
Purity
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)

CD28-Fc, Active Protein (Cat# AAA692481)

Full Name
Human CD28-Fc
Gene Names
CD28; Tp44
Reactivity
Human
Purity
> 95% by SDS-PAGE & HPLC analysis
Pricing
$250/0.02 mg | $395/0.1 mg | $1,565/5x0.1 mg
Testing Data #1 (Detection of Mouse anti Rabbit CD44 (MBS224526) in a flow cytometric analysis of rabbit peripheral blood lymphocytes using Goat anti Rabbit IgG H+L:FITC (MBS235142))

IgG, Polyclonal Antibody (Cat# AAA235142)

Full Name
GOAT ANTI MOUSE IgG (H/L):FITC (MULTI SPECIES ADSORBED)
Applications
FC/FACS
Pricing
$300/0.5 mg | $1,200/5x0.5 mg
Western Blot (WB) (Figure 1. Western blot analysis of ICOS using anti-ICOS antibody (MBS1750365).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: mouse stomach tissue lysates,Lane 2: mouse spleen tissue lysates,Lane 3: mouse thymus tissue lysates,Lane 4: mouse small intestine tissue lysates,Lane 5: rat thymus tissue lysates,Lane 6: human placenta tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ICOS antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ICOS at approximately 20KD. The expected band size for ICOS is at 22KD. )

ICOS, Polyclonal Antibody (Cat# AAA1750365)

Full Name
Anti-ICOS Picoband antibody
Gene Names
Icos; H4; CCLP; AILIM; CRP-1; Ly115
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
Applications
EIA, IHC, WB
Pricing
$345/0.1 mg | $1,485/5x0.1 mg

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