Rabbit anti-Human, Mouse SUSD3 Polyclonal Antibody | anti-SUSD3 antibody

SUSD3 Antibody

Cat. Num. AAA154369

• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column.
• Synonyms: SUSD3; Polyclonal Antibody; SUSD3 Antibody; Sushi domain containing 3; anti-SUSD3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SUSD3 antibody is human and mouse reactive. Multiple isoforms of SUSD3 are known to exist.
• Purity/Purification: Affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 255

• Applicable Applications for anti-SUSD3 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: SUSD3 antibody can be used for the detection of SUSD3 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL.
• Immunogen: SUSD3 antibody was raised against a 16 amino acid peptide from near the center of human SUSD3.
• Buffer: Supplied in PBS containing 0.02% sodium azide.
• Positive Control 1: 3T3 Cell Lysate
• Conjugate: Unconjugated
• Preparation and Storage: Store at 4 degree C for three months and -20 degree C, stable for up to one year.

Western Blot (WB)

(Western blot analysis of SUSD3 in HeLa cell lysate with SUSD3 antibody at 1 μg/ml.)

Immunocytochemistry (IHC)

(Immunocytochemistry of SUSD3 in 3T3 cells with SUSD3 antibody at 5 μg/ml.)

Related Product Information for anti-SUSD3 antibody

Little is known of the function of the sushi domain containing 3 protein (SUSD3), but its expression has been reported in estrogen receptor-alpha (ERalpha)-positive breast tumors with decreased expression reported in aggressive malignant tumors (1,2). Recently, SUSD3 has been found to promote estrogen-dependent cell proliferation and may regulate cell-cell and cell-substrate interactions and migration in breast cancer (3). Furthermore, elevated SUSD3 mRNA levels were observed in aromatase inhibitor-responsive breast tumors, suggesting that it may also serve as a novel predictor of response to endocrine therapy and a potential therapeutic target (3).

Product Categories/Family for anti-SUSD3 antibody

Cell Cycle; Cancer

References

Abba MC, Hu Y, Sun H, et al. Gene expression signature of estrogen receptor alpha status in breast cancer. BMC Genomics 2005; 6:37. Parris TZ, Danielsson A, Nemes S, et al. Clinical implications of gene dosage and gene expression patterns in diploid breast cancer. Clin. Cancer Res. 2010; 16:3860-74. Moy I, Todorovic V, Dubash AD, et al. Estrogen-dependent sushi domain containing 3 regulates cytoskeleton organization and migration in breast cancer cells. Oncogene 2015; 34:323-33.

NCBI and Uniprot Product Information

• NCBI GI #: 21450717
• NCBI GeneID: 203328
• NCBI Accession #: NP_659443
• NCBI GenBank Nucleotide #: NM_145006.3
• UniProt Accession #: Q96L08; Q49AA6; Q6UXV7
• Molecular Weight: Predicted: 16, 22, 23, 28 kDa; Observed: 23 kDa
• NCBI Official Full Name: sushi domain-containing protein 3 isoform a
• NCBI Official Synonym Full Names: sushi domain containing 3
• NCBI Official Symbol: SUSD3
• NCBI Protein Information: sushi domain-containing protein 3
• UniProt Protein Name: Sushi domain-containing protein 3
• Protein Family: Sushi domain-containing protein
• UniProt Gene Name: SUSD3
• UniProt Entry Name: SUSD3_HUMAN

Uniprot Description

SUSD3: 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 9q22.31

Cellular Component: integral to membrane; plasma membrane