Rat CD28 Monoclonal Antibody | anti-CD28 antibody

RAT ANTI HUMAN CD28:Low Endotoxin

Cat. Num. AAA211263

• Gene Names: CD28; Tp44
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Functional Assay
• Synonyms: CD28; Monoclonal Antibody; RAT ANTI HUMAN CD28:Low Endotoxin; anti-CD28 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: YTH913.12
• Form/Format: Low Endotoxin; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 234

• Applicable Applications for anti-CD28 antibody: Immunohistology Frozen, Flow cytometry (FC/FACS), Functional Assays (FN)
• Application Notes: Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. ; Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Buffer Solution: Phosphate buffered saline
• Target Species: Human
• Preparation and Storage: Store at -20 degree C only. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin (MBS210509))

Testing Data

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28:RPE (MBS213865))

Testing Data

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Alexa Fluor 647 (MBS2141859A647))

Testing Data

(Staining of human peripheral blood lymphocytes with Rat anti Human CD28 (MBS213120))

Related Product Information for anti-CD28 antibody

Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompson et al. 1989), responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Viola et al.1996). CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummel et al. 1995) Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauri et al 1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived KK cells using clone YTH913.12 (Wilson et al. 1999).

NCBI and Uniprot Product Information

• NCBI GI #: 340545508
• NCBI GeneID: 940
• NCBI Accession #: NP_001230006.1
• NCBI GenBank Nucleotide #: NM_001243077.1
• UniProt Accession #: P10747; Q13964; Q52M23; Q70WG0; Q8NI54; Q8NI55; Q8NI56; Q8WXJ2; Q9BYV0; A8KAC1
• Molecular Weight: 26,186 Da
• NCBI Official Full Name: T-cell-specific surface glycoprotein CD28 isoform 2
• NCBI Official Synonym Full Names: CD28 molecule
• NCBI Official Symbol: CD28
• NCBI Official Synonym Symbols: Tp44
• NCBI Protein Information: T-cell-specific surface glycoprotein CD28; CD28 antigen
• UniProt Protein Name: T-cell-specific surface glycoprotein CD28
• Protein Family: T-cell-specific surface glycoprotein
• UniProt Gene Name: CD28
• UniProt Entry Name: CD28_HUMAN

NCBI Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

Uniprot Description

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described.

Protein type: Cell surface; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane

Molecular Function: identical protein binding; protein binding; protease binding; SH3/SH2 adaptor activity; coreceptor activity

Biological Process: positive regulation of isotype switching to IgG isotypes; nerve growth factor receptor signaling pathway; positive regulation of translation; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine biosynthetic process; T cell receptor signaling pathway; positive regulation of inflammatory response to antigenic stimulus; cell surface receptor linked signal transduction; positive regulation of T cell proliferation; regulatory T cell differentiation; epidermal growth factor receptor signaling pathway; positive regulation of viral genome replication; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of mitosis; negative thymic T cell selection; regulation of defense response to virus by virus; humoral immune response; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; T cell costimulation; innate immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of alpha-beta T cell proliferation