CD80 (B7-1) Active Protein | CD80 active protein

Human CD80 Recombinant (B7-1)

Cat. Num. AAA553137

• Purity: >90%, as determined by SDS-PAGE and HPLC
• Synonyms: CD80 (B7-1); Human CD80 Recombinant (B7-1); Human CD80 Recombinant (B7-1) ; CD80; B7-1; CD80 active protein

• Host: HEk293 Cells
• Purity/Purification: >90%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant CD80 was lyophilized from 0.2um filtered 50mM Tris, 100mM NaCl, pH 8.0.
• Sequence Length: 214

• Domain: Ig-like C2
• Host Note: Optimized DNA sequence encoding Human extracellular domain of human CD80 including a C-terminal 6His tag was expressed in HEK293 cells.
• Biological Activity: The activity was tested by immobilized recombinant CD80 (1ug/ml) ability to bind recombinant human CD28 in functional ELISA.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Molecular Weight Note: Recombinant CD80 is a monomer protein consisting of 214 amino acid residue subunits, due to glycosylation migrates as an approximately 45 kDa protein on SDS-PAGE.
• Preparation and Storage: Recombinant CD80, as supplied, can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C to -70 degree C for twelve months.; ; Avoid repeated freeze/thaw cycles.

Related Product Information for CD80 active protein

CD80 is a monomeric surface protein found on activated APCs. The extracellular region of CD80 consists of two highly glycosylated Ig- like extracellular domains, which have defined regions for binding its ligands, CD28 or CTLA-4, on T cells.One ligand of CD80, CD28, provides critical costimulatory signals to activated T cells to increase cytokine production, cell proliferation, and cell viability, while the other ligand, CTLA-4, may mediate a growth-inhibitory signal.The same regions of CD80 are involved in binding to both CD28 and CTLA-4, but CTLA-4 binds to CD80 with about a 20-fold higher avidity than CD28 does. This suggests that low levels of CTLA-4 expressed on the cell surface can compete for CD80 binding even when CD28 is expressed at much higher levels.

Product Categories/Family for CD80 active protein

Receptors CD

References

• tumor cell programmed death ligand 1-mediated t cell suppression is overcome by coexpression of CD80 .J. Immunol., Jun 2011; 186: 6822 - 6829.
• toll-like receptor 3 ligands induce CD80 expression in human podocytes via an nf-b-dependent pathway .Nephrol. Dial. Transplant., May 2011; 10.1093/ndt/gfr271.
• trans-endocytosis of CD80 and cd86: a molecular basis for the cell-extrinsic function of ctla-4 .Science, Apr 2011; 332: 600 - 603.
• critical role of transcription factor pu.1 in the expression of CD80 and cd86 on dendritic cells .Blood, Feb 2011; 117: 2211 - 2222.
• enhancement of suboptimal cd8 cytotoxic t cell effector function in vivo using antigen-specific CD80 defective t cells .J. Immunol., Jan 2011; 186: 291 - 304.