Human CD80 molecule ELISA Kit | CD80 elisa kit

Human Soluble Cluster of Differentiation 80, sCD80 ELISA Kit

Cat. Num. AAA705535

• Gene Names: CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• Reactivity: Human
• Synonyms: CD80 molecule; Human Soluble Cluster of Differentiation 80; sCD80 ELISA Kit; Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit; CD28LG; CD28LG1; LAB7; B-lymphocyte activation antigen B7; CD80 antigen; CD80 antigen (CD28 antigen ligand 1; B7-1 antigen) ; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CD80 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human sCD80. No significant cross-reactivity or interference between human sCD80 and analogues was observed.
• Sequence Length: 288

• Samples: Serum, plasma, cell culture supernates
• Assay Type: Sandwich
• Detection Range: 125 pg/ml -8000 pg/ml.
• Sensitivity: The minimum detectable dose of human sCD80 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CD80 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD80 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD80 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD80 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD80 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 4885123
• NCBI GeneID: 941
• NCBI Accession #: NP_005182.1
• NCBI GenBank Nucleotide #: NM_005191.3
• UniProt Accession #: P33681; Q5DTA9; Q5DTB0
• Molecular Weight: 33,048 Da
• NCBI Official Full Name: T-lymphocyte activation antigen CD80
• NCBI Official Synonym Full Names: CD80 molecule
• NCBI Official Symbol: CD80
• NCBI Official Synonym Symbols: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
• NCBI Protein Information: T-lymphocyte activation antigen CD80; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen)
• UniProt Protein Name: T-lymphocyte activation antigen CD80
• Protein Family: T-lymphocyte activation antigen
• UniProt Gene Name: CD80
• UniProt Synonym Gene Names: CD28LG; CD28LG1; LAB7; B7
• UniProt Entry Name: CD80_HUMAN

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Function: Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.1 PublicationManual assertion based on experiment in:Ref.8