Quality Control (Western blot analysis of immunized recombinant protein, using anti-ABCF2 monoclonal antibody. )
Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Western Blot (WB) (Detection of ABCF2 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)
Immunoprecipitation (IP) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-ABCF2 mAb. [Lot No. 2001C1-1]Predicted molecular weight: 71 kDa)
Flow Cytometry (FC/FACS) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-ABCF2 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2001C1-1])
Western Blot (WB) (All lanes: Phosphatidylserine synthase 1 antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 30 kDaObserved band size: 30kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human colorectal carcinoma using MBS7000195 at dilution of 1:100)
Western Blot (WB) (Western blotAll lanes: Endoplasmic reticulum-Golgi intermediate compartment protein 3 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:293T whole cell lysateLane 3:Hela whole cell lysateLane 4:mcf-7 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 44,27 kDaObserved band size: 43 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human rectum tissue using MBS7000269 at dilution 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of Hela cells using MBS7000269 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human pancreas using MBS7001732 at dilution 1:100)
Western Blot (WB) (Western BlotPositive WB detected in: U87 whole cell lysate,Rat heart tissue,Mouse liver tissueAll lanes: GRIN2A antibody at 3.2ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 166,145 KDaObserved band size: 166 KDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human brain tissue at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of HepG2 cells at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: Aspartate aminotransferase, mitochondrial antibody at 2ug/mllane 1:EC109 whole cell lysatelane 2:293T whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 48,44 kDaObserved band size: 47 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human liver tissue using MBS7006066 at dilution 1:100)
Immunohistochemistry (IHC) (Immunofluorescent analysis of MCF-7 cells using MBS7006066 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (All lanes: Long-chain fatty acid transport protein 4 antibody at 8ug/ml+mouse heart tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 73,27 kDaObserved band size: 73 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate tissue using MBS968905 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human placenta tissue using MBS968905 at dilution 1:100)
Immunofluorescence (IF) (MBS9603737 staining Hela by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9603737 1:200) and mouse anti-beta tubulin Ab(MBS9610328 1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Western Blot (WB) (Western blot analysis of extracts from HepG2, using xCT Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry-Paraffin (MBS9603737at 1/100 staining human mammary cancer tissue byIHC-P. The sample was formaldehyde fixed and a heatmediated antigen retrieval step in citrate buffer wasperformed. The sample was then blocked and incubated withthe antibody for 1.5 hours at 22°C. An HRP conjugated goatanti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney using MBS7003777 at dilution of 1:20)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7003777 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: Acetylcholine receptor subunit alpha antibody at 2ug/ml+rat skeletal muscleSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 55,52 kDaObserved band size: 55 kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using MBS7003178 at dilution of 1:100)
Western Blot (WB) (Western blotAll lanes: ATP-sensitive inward rectifier potassium channel 1 antibody at 2ug/mlLane 1:HepG2 whole cell lysateLane 2:Hela whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 45,43 kDaObserved band size: 45 kDa)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7006796 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial Antibody at 2ug/ml+293T whole cell lysateat 20 ugSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 73,68,57 kDaObserved band size: 73 kDa,60 kDa)
Immunocytochemistry (ICC) (Immunocytochemistry analysis of human skeletal muscle tissue using MBS7004714 at dilution of 1:100)
Immunohistochemistry (IHC) (Immunocytochemistry analysis of human lung tissue using MBS7004714 at dilution of 1:100)
Immunofluorescence (IF) (Immunofluorescent analysis of PC3 cells using MBS7004714 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blotAll lanes: NADH dehydrogenase flavoprotein 2, mitochondrial antibody at 2ug/mlLane 1: EC109 whole cell lysateLane 2: 293T whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilutionPredicted band size: 27 kDaObserved band size: 27kDaAdditional bands at: 40kDa)
Immunohistochemistry (IHC) (Amyloid (Abeta) staining on Alzheimer's Disease-Hypothalamus. Tissue pretreated with Citrate, pH 6.0. beta Amyloid antibody was diluted to 1:80. Immunoreactivity is seen as staining on plaque deposits (dark brown).)
Immunohistochemistry (IHC) (Amyloid (Abeta) staining on Alzheimer's Disease-Hypothalamus (left). Tissue pretreated with Citrate, pH 6.0. beta Amyloid antibody diluted to 1:80. Immunoreactivity is seen as cerebral vascular amyloid deposition in the walls of blood vessels (brown). Negative Control staining on Alzheimer's Disease-Hypothalamus (right). Tissue pretreated with Citrate, pH 6.0.)
Immunohistochemistry (IHC) (Negative Control staining on Alzheimer's Disease-Hypothalamus. Tissue pretreated with Citrate, pH 6.0. Negative Control Reagent, IHC-Select Detection with HRP-DAB.)
Western Blot (WB) (APP antibody western blot of endogenous APPs from SH-SY5Y conditioned medium.)
Western Blot (WB) (Western blotAll lanes: Ubiquitin-conjugating enzyme E2 D1 antibody at 2ug/mlLane 1:Hela whole cell lysateLane 2:NIH3T3 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 17kDaObserved band size: 17kDa)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human prostate cancer using MBS7006023 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using MBS7006023 at dilution 1:100)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embeded human rectum tissue using MBS7001038 at dilution of 1:50)
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7001038 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Western Blot (WB) (Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysates,Lane 2: mouse liver tissue lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- SLC10A1 antigen affinity purified polyclonal antibody (MBS177905) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit ( MBS176460 ) with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
Immunofluorescence (IF) (IF analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).SLC10A1 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- SLC10A1 Antibody (MBS177905) overnight at 4°C. DyLight®550 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Immunofluorescence (IF) (IF analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).SLC10A1 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/mL rabbit anti- SLC10A1 Antibody (MBS177905) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
Flow Cytometry (FC/FACS) (Flow Cytometry analysis of BRL cells using anti-SLC10A1 antibody (MBS177905). Overlay histogram showing BRL cells stained with MBS177905 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC10A1 Antibody (MBS177905,1ug/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Immunohistochemistry (IHC) (IHC analysis of SLC10A1 using anti-SLC10A1 antibody (MBS177905).SLC10A1 was detected in frozen section of mouse liver tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC10A1 Antibody (MBS177905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)( MBS176451 ) with DAB as the chromogen.)
Immunohistochemistry (IHC) (IHC analysis of SLC10A1 using anti-SLC10A1 antibody (MBS177905). SLC10A1 was detected in paraffin-embedded section of Human Liver Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti- SLC10A1 Antibody (MBS177905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen.)
Immunohistochemistry (IHC) (IHC analysis of SLC10A1 using anti-SLC10A1 antibody (MBS177905).SLC10A1 was detected in paraffin-embedded section of Rat Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC10A1 Antibody (MBS177905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen.)
Immunohistochemistry (IHC) (IHC analysis of SLC10A1 using anti-SLC10A1 antibody (MBS177905).SLC10A1 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SLC10A1 Antibody (MBS177905) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(MBS176451) with DAB as the chromogen.)
Western Blot (WB) (Western blot analysis of SLC10A1 using anti- SLC10A1 antibody (MBS177905).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates (positive control), Lane 2: rat kidney tissue lysates, (negative control)After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC10A1 antigen affinity purified polyclonal antibody (MBS177905) at 0.25
ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SLC10A1 at approximately 50KD. The expected band size for SLC10A1 is at 38KD.)
Western Blot (WB) (Western blot detection of EGFR in A549, MDA-MB-468 and COS7 cell lysates using EGFR mouse mAb (dilution 1:1000).Predicted band size:134 Kda.Observed band size:175KDa.)
Immunocytochemistry (ICC) (Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).)
Immunoprecipitation (IP) (Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.)
Immunocytochemistry (ICC) (Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).)
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