Typical Testing Data/Standard Curve (for reference only)
Mouse glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) ELISA Kit | GOT2 elisa kit
Mouse Aspartate Aminotransferase, AST ELISA Kit
Gene Names
Got2; Got-2; mAspAT; FABP-pm; AL022787
Reactivity
Mouse
Synonyms
glutamic-oxaloacetic transaminase 2; mitochondrial (aspartate aminotransferase 2); Mouse Aspartate Aminotransferase; AST ELISA Kit; Mouse Aspartate Aminotransferase (AST) ELISA kit; FLJ40994; KAT4; KATIV; mitAAT; aspartate aminotransferase 2; kynurenine aminotransferase IV; GOT2 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mouse GOT2. No significant cross-reactivity or interference between Mouse GOT2 and analogues was observed.
Sequence Length
430
Samples
Serum, plasma, cell culture supernates
Detection Range
1.56 mIU/ml-100 mIU/ml
Sensitivity
0.39 mIU/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Transport
Protein Biological Process 3
Lipid transport
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for GOT2 elisa kit
Introduction: Aspartate transaminase (AST) also called serum glutamic oxaloacetic transaminase (SGOT) or aspartate aminotransferase (ASAT/AAT) is similar to alanine transaminase (ALT) in that it is another enzyme associated with liver parenchymal cells. It facilitates the conversion of aspartate and alpha-ketoglutarate to oxaloacetate and glutamate, and vice-versa. AST is normally found in red blood cells, liver, heart, muscle tissue, pancreas, and kidneys. Low levels of AST are normally found in the blood. When body tissue or an organ such as the heart or liver is diseased or damaged, additional AST is released into the bloodstream. The amount of AST in the blood is directly related to the extent of the tissue damage. After severe damage, AST levels rise in 6 to 10 hours and remain high for about 4 days.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for AST and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,411 Da
NCBI Official Full Name
aspartate aminotransferase, mitochondrial
NCBI Official Synonym Full Names
glutamate oxaloacetate transaminase 2, mitochondrial
NCBI Official Symbol
Got2
NCBI Official Synonym Symbols
Got-2; mAspAT; FABP-pm; AL022787
NCBI Protein Information
aspartate aminotransferase, mitochondrial; FABP-1; FABPpm; transaminase A; fatty acid-binding protein; kynurenine aminotransferase 4; kynurenine aminotransferase IV; kynurenine--oxoglutarate transaminase 4; kynurenine--oxoglutarate transaminase IV; mitochondrial aspartate aminotransferase; plasma membrane fatty acid binding protein; plasma membrane-associated fatty acid-binding protein
UniProt Protein Name
Aspartate aminotransferase, mitochondrial
UniProt Gene Name
Got2
UniProt Synonym Gene Names
Got-2; mAspAT; FABP-1; FABPpm
UniProt Entry Name
AATM_MOUSE
Uniprot Description
Function: Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.2 PublicationsManual assertion based on experiment in:Ref.7
Catalytic activity: L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.2 PublicationsManual assertion based on experiment in:Ref.9
Cofactor: Pyridoxal phosphate.1 PublicationManual assertion based on experiment in:Ref.13
Research Articles on GOT2
Similar Products
Product Notes
The Mouse GOT2 got2 (Catalog #AAA703700) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA703700 ELISA Kit recognizes Mouse GOT2. It is sometimes possible for the material contained within the vial of "glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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