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1425 results for " Rac1" - showing 1-50
Ras-Related C3 Botulinum Toxin Substrate 1, ELISA Kit (Cat# AAA19715)
Full Name
Human Ras-Related C3 Botulinum Toxin Substrate 1 ELISA Kit
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Ras-Related C3 Botulinum Toxin substrate 1, Recombinant Protein (Cat# AAA143122)
Full Name
Recombinant Human Ras-Related C3 Botulinum Toxin substrate 1
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Purity
Greater than 95.0% as determined by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $1,925/1 mg | $8,435/5x1 mg
Testing Data
RhoA/Rac1/CDC42 Activation, Assay Kit (Cat# AAA168321)
Full Name
RhoA/Rac1/CDC42 Activation Assay Combo Kit
Pricing
$1,175/3x10 Assays
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MUSK Antibody (MBS9205728) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
MUSK, Polyclonal Antibody (Cat# AAA9205728)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
WB, EIA, FC, IF
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Typical Testing Data/Standard Curve (for reference only)
Rac1, ELISA Kit (Cat# AAA2510810)
Full Name
Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit
Reactivity
Human
Pricing
$440/48-Strip-Wells | $525/96-Strip-Wells | $2,215/5x96-Strip-Wells | $3,965/10x96-Strip-Wells | $250/24-Strip-Wells
Western Blot (WB)
(Western Blot analysis of 293T and K562 cell using PAK1 Polyclonal Antibody at dilution of 1:700)
PAK1, Polyclonal Antibody (Cat# AAA2517277)
Full Name
PAK1 Polyclonal Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
Antigen affinity purification
Pricing
$125/0.02 mL | $405/0.2 mL | $255/0.12 mL | $180/0.06 mL | $1,825/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using RAC1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
RAC1, Polyclonal Antibody (Cat# AAA9127955)
Full Name
RAC1 Polyclonal Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Typical Testing Data/Standard Curve (for reference only)
Ras-related C3 Botulinum Toxin Substrate 1, ELISA Kit (Cat# AAA749874)
Full Name
Mouse Ras-related C3 Botulinum Toxin Substrate 1 ELISA Kit
Gene Names
Rac1; AL023026; D5Ertd559e
Reactivity
Mouse
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210328)
Full Name
MOUSE ANTI V5-TAG:Biotin
Applications
EIA, WB
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
Testing Data
Rac1/Cdc42 Activation, Assay Kit (Cat# AAA168431)
Full Name
Rac1/Cdc42 Activation Assay Combo Kit
Pricing
$1,175/2x20 Assays
Western Blot (WB)
(Western Blot: Sample: Human Jurkat cell lysate; Primary Ab: 5ug/ml Rabbit Anti-Human PAK2 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
p21 Protein Activated Kinase 2, Polyclonal Antibody (Cat# AAA2026762)
Full Name
Polyclonal Antibody to p21 Protein Activated Kinase 2 (PAK2)
Gene Names
PAK2; PAK65; PAKgamma
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western Blot: Lane1: Human Hela Cells; Lane2: Human Jurkat Cells; Lane3: Rat Brain Tissue.)
p21 Protein Activated Kinase 1, Polyclonal Antibody (Cat# AAA2026367)
Full Name
Polyclonal Antibody to p21 Protein Activated Kinase 1 (PAK1)
Gene Names
PAK1; PAKalpha
Reactivity
Human
Applications
WB, ICC, IHC, EIA
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
p21 Protein Activated Kinase 4 (PAK4), Polyclonal Antibody (Cat# AAA2013051)
Full Name
Polyclonal Antibody to p21 Protein Activated Kinase 4 (PAK4)
Reactivity
Human
Applications
ICC, IHC, EIA, WB
Purity
Affinity Chromatography
Pricing
$165/0.1 mL | $205/0.2 mL | $345/0.5 mL | $425/1 mL | $1,220/5x1 mL
PAK2, ELISA Kit (Cat# AAA9501638)
Full Name
Phospho-PAK2 (Ser141) Colorimetric Cell-Based ELISA Kit
Gene Names
PAK2; PAK65; PAKgamma
Reactivity
Human, Mouse, Rat
Pricing
$575/2x96 Wells | $2,580/10x96 Wells | $5,065/20x96 Wells
Western Blot (WB)
(Western blotAll lanes: ARFIP2 antibody at 2ug/ml+mouse brain tissueSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 38,30,34 kDaObserved band size: 38 kDa)
ARFIP2, Polyclonal Antibody (Cat# AAA7002089)
Full Name
ARFIP2 Antibody
Reactivity
Human, mouse
Applications
EIA, WB, IHC
Purity
Affinity Purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with STAT3 Mouse Monoclonal Antibody (STAT3/2409).)
STAT3/Signal Transducer and Activator of Transcription 3, Monoclonal Antibody (Cat# AAA4380497)
Full Name
STAT3/Signal Transducer and Activator of Transcription 3
Gene Names
STAT3; APRF; HIES; ADMIO; ADMIO1
Reactivity
Human. Others not tested.
Applications
IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
RAC1, cDNA Clone (Cat# AAA1269588)
Full Name
RAC1 cDNA Clone
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Western Blot (WB)
(Western Blot analysis of various cells with Rac1/2/3/CDC42 Polyclonal Antibody.)
Rac1/2/3/CDC42, Polyclonal Antibody (Cat# AAA2536486)
Full Name
Rac1/2/3/CDC42 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
Antigen Affinity Purified
Pricing
$130/0.02 mL | $395/0.2 mL | $250/0.12 mL | $170/0.06 mL | $1,715/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7046153 at dilution of 1:100)
RAC1, Polyclonal Antibody (Cat# AAA7046153)
Full Name
RAC1 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
EIA, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
Western Blot (WB)
(Western BlotPositive WB detected in:Mouse lung tissue,Mouse skeletal muscle tissueAll lanes: PAK3 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 63,61,65 kDaObserved band size: 63 kDa)
PAK3, Polyclonal Antibody (Cat# AAA7048826)
Full Name
PAK3 Antibody
Gene Names
PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
>95%, Protein G purified
Pricing
$180/0.05 mg | $260/0.1 mg | $1,160/5x0.1 mg
PAK6, cDNA Clone (Cat# AAA1269516)
Full Name
PAK6 cDNA Clone
Gene Names
PAK6; PAK5
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
PAK4, cDNA Clone (Cat# AAA1266670)
Full Name
PAK4 cDNA Clone
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
RAC1, cDNA Clone (Cat# AAA1271762)
Full Name
RAC1 cDNA Clone
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lymphoma reacted with MERTK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
MERTK, Polyclonal Antibody (Cat# AAA9200853)
Full Name
MERTK Antibody
Gene Names
MERTK; MER; RP38; c-Eyk; c-mer; Tyro12
Reactivity
Human
Applications
EIA, IHC, FC/FACS, IF, WB
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA214958)
Full Name
MOUSE ANTI V5-TAG:HRP
Applications
EIA, WB
Pricing
$490/0.1 mg | $2,060/5x0.1 mg
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA210664)
Full Name
MOUSE ANTI V5-TAG:FITC
Applications
IF
Pricing
$445/0.1 mg | $1,845/5x0.1 mg
PAK7, cDNA Clone (Cat# AAA1268108)
Full Name
PAK7 cDNA Clone
Gene Names
PAK5; PAK7
Pricing
$845/5x0.01 mg Plasmid + 5x0.2 mL Glycerol-Stock | $190/0.01 mg Plasmid + 0.2 mL Glycerol-Stock
Flow Cytometry (FC)
(Flow cytometric analysis of U-87 MG cells using RAC1(green) compared to an isotype control of mouse IgG2b(blue). AP20600c was diluted at 1:100 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)
RAC1, Monoclonal Antibody (Cat# AAA9200589)
Full Name
RAC1 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, rat (Predicted Reactivity: Bovine, Mouse)
Applications
WB, EIA, FC, IHC
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of A431 cells, using Phospho-Rac1/cdc42 (Ser71) Antibody.)
Rac1/cdc42, Polyclonal Antibody (Cat# AAA9600721)
Full Name
Phospho-Rac1/cdc42 (Ser71) Antibody
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded M. skeletal muscle section using Musk Antibody. MBS9200303 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Musk, Monoclonal Antibody (Cat# AAA9200303)
Full Name
Musk Antibody
Gene Names
Musk; Mlk; Mdk4; Nsk1; Nsk2; Nsk3
Reactivity
Mouse, Rat
Applications
EIA, IHC, WB
Purity
This antibody is purified through a protein G column, followed by dialysis against PBS.
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of Rac 1 expression in BT474 (A), mouse liver (B), rat brain (C) whole cell lysates.)
Rac 1, Polyclonal Antibody (Cat# AAA8245759)
Full Name
Anti-Rac 1 Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$200/0.05 mL | $245/0.1 mL | $325/0.2 mL | $1,410/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA216888)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$1,350/2 mg | $5,915/5x2 mg
Immunohistochemistry (IHC)
(MBS9607731 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PAK4, Polyclonal Antibody (Cat# AAA9607731)
Full Name
PAK4 Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Testing Data
Rac1 G15A Agarose Beads (Active Rac-GEF), Accessory Reagent (Cat# AAA168525)
Full Name
Rac1 G15A Agarose Beads (Active Rac-GEF)
Purity
Purity >90% by SDS-PAGE and Coomassie blue staining. Specifically interacts and precipitaes active Rac-GEF from cell lysate.
Pricing
$750/0.8 mg | $3,220/5x0.8 mg
Immunohistochemistry (IHC)
(MBS9601974 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PAK1, Polyclonal Antibody (Cat# AAA9601974)
Full Name
PAK1 Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of CYFIP1 using HT-29 whole cell lysates)
CYFIP1, Polyclonal Antibody (Cat# AAA9602918)
Full Name
CYFIP1 Antibody
Gene Names
CYFIP1; SHYC; SRA1; SRA-1; P140SRA-1
Reactivity
Human
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9604417 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PAK1/2/3, Polyclonal Antibody (Cat# AAA9604417)
Full Name
Phospho-PAK1/2/3 (Ser144+Ser141+Ser139) Antibody
Gene Names
PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600708 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PAK1, Polyclonal Antibody (Cat# AAA9600708)
Full Name
Phospho-PAK1 (Ser199/204)/PAK2 (Ser192/197) Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Sample: Recombinant Rac1, Human.)
Ras Related C3 Botulinum Toxin Substrate 1, Polyclonal Antibody (Cat# AAA2028730)
Full Name
Polyclonal Antibody to Ras Related C3 Botulinum Toxin Substrate 1 (Rac1)
Gene Names
RAC1; MIG5; MRD48; Rac-1; TC-25; p21-Rac1
Reactivity
Human
Applications
WB, ICC, IHC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Pricing
$175/0.1 mL | $220/0.2 mL | $375/0.5 mL | $465/1 mL | $1,295/5x1 mL
Western Blot (WB)
(Western blot analysis USP6 using HeLa whole cell lysates)
USP6, Polyclonal Antibody (Cat# AAA9610309)
Full Name
USP6 Antibody
Gene Names
USP6; HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
Reactivity
Human, Mouse, Rat
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of PAK5/6 using COLO whole cell lysates)
PAK5/6, Polyclonal Antibody (Cat# AAA9602192)
Full Name
PAK5/6 Antibody
Gene Names
PAK5; PAK7
Reactivity
Human
Applications
WB, IF, ICC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis on rat heart cell lysate using Phospho-PAK3(Ser154) Antibody.The lane on the left is treated with the antigen-specific peptide.)
PAK3, Polyclonal Antibody (Cat# AAA9600018)
Full Name
Phospho-PAK3(Ser154) Antibody
Gene Names
PAK3; ARA; bPAK; MRX30; MRX47; OPHN3; PAK-3; PAK3beta; beta-PAK
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts from rat brain cells, using PAK7 antibody.The lane on the left is treated with the antigen-specific peptide.)
PAK7, Polyclonal Antibody (Cat# AAA9606241)
Full Name
PAK7 Antibody
Gene Names
PAK5; PAK7
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9607815 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PARD6A, Polyclonal Antibody (Cat# AAA9607815)
Full Name
PARD6A Antibody
Gene Names
PARD6A; PAR6; PAR6C; TAX40; PAR-6A; TIP-40; PAR6alpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9603682 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PIP5K1A, Polyclonal Antibody (Cat# AAA9603682)
Full Name
PIP5K1A Antibody
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(MBS9600709 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
PAK2, Polyclonal Antibody (Cat# AAA9600709)
Full Name
Phospho-PAK2 (Ser20) Antibody
Gene Names
PAK2; PAK65; PAKgamma
Reactivity
Human, Mouse, Rat
Applications
WB, IHC
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $310/0.2 mL | $1,330/5x0.2 mL
Western Blot (WB)
(Western blot analysis of PAK1 phosphorylation expression in mouse brain tissue lysates, The lane on the right is treated with the antigen-specific peptide.)
PAK1, Polyclonal Antibody (Cat# AAA9601193)
Full Name
Phospho-PAK1 (Thr212) Antibody
Gene Names
PAK1; PAKalpha
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, ICC, EIA
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using MBS7603298 (RAC1 Antibody) at dilution of 1:400)
Rac1, Monoclonal Antibody (Cat# AAA7603298)
Full Name
Rac1 Mouse Monoclonal
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC, IP, IF
Purity
Purity: >=95% as determined by SDS-PAGE
Purification: Protein A+G purification
Purification: Protein A+G purification
Pricing
$320/0.1 mg | $1,360/5x0.1 mg
Western Blot (WB)
(Western blot analysis of extracts of A-431 cells, using Phospho-RAC1-S71 antibody at 1:1000 dilution. A431 cells were treated by EGF (100ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.)
RAC1-S71, Antibody (Cat# AAA125366)
Full Name
Phospho-RAC1-S71 Polyclonal Antibody
Gene Names
RAC1; MIG5; Rac-1; TC-25; p21-Rac1
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $250/0.05 mL | $350/0.1 mL | $525/0.2 mL | $2,210/5x0.2 mL
