Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using MBS7603298 (RAC1 Antibody) at dilution of 1:400)
Mouse Rac1 Monoclonal Antibody | anti-Rac1 antibody
Rac1 Mouse Monoclonal
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Purity: >=95% as determined by SDS-PAGEPurification: Protein A+G purification
Synonyms
Rac1; Monoclonal Antibody; Rac1 Mouse Monoclonal; MIG5; p21 Rac1; RAC1; RAC1b; Ras like protein TC25; TC 25; TC25; anti-Rac1 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D9
Specificity
Other species are not tested. Please decide the specificity by homology.
Purity/Purification
Purity: >=95% as determined by SDS-PAGE
Purification: Protein A+G purification
Purification: Protein A+G purification
Form/Format
Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3
Sequence Length
192
Applicable Applications for anti-Rac1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF).
Application Notes
WB: 1:1000-1:4000
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:50-1:500
IP: 1:200-1:2000
IHC: 1:50-1:500
IF: 1:50-1:500
Immunogen
ras-related C3 botulinum toxin substrate 1(rho family, small GTP binding protein Rac1)
Preparation and Storage
-20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using MBS7603298 (RAC1 Antibody) at dilution of 1:400)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using MBS7603298 (RAC1 Antibody) at dilution of 1:400)
Immunofluorescence (IF)
(Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using MBS7603298 (Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using MBS7603298 (Rac1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L))
Western Blot (WB)
(HSCT6 cells were subjected to SDS PAGE followed by western blot with MBS7603298 (Rac1 antibody) at dilution of 1:2000)
Western Blot (WB)
(HSCT6 cells were subjected to SDS PAGE followed by western blot with MBS7603298 (Rac1 antibody) at dilution of 1:2000)
Related Product Information for anti-Rac1 antibody
RAC1b, also named as TC25, belongs to the small GTPase superfamily and Rho family. RAC1 is a plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, it binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
21KD
NCBI Official Full Name
Rac1
UniProt Protein Name
Ras-related protein Rac1
Protein Family
UniProt Gene Name
Rac1
UniProt Synonym Gene Names
RacA
UniProt Entry Name
RAC1_DROME
Uniprot Description
Involved in axon outgrowth and myoblast fusion. Plays a role in regulating dorsal closure during embryogenesis. Involved in integrin alpha-PS3/beta-nu-mediated phagocytosis of Gram-positive S.aureus by hemocytes.
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Product Notes
The Rac1 rac1 (Catalog #AAA7603298) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Rac1 Mouse Monoclonal reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Rac1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF). WB: 1:1000-1:4000 IP: 1:200-1:2000 IHC: 1:50-1:500 IF: 1:50-1:500. Researchers should empirically determine the suitability of the Rac1 rac1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Rac1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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