Rabbit USP6 Polyclonal Antibody | anti-USP6 antibody

USP6 Antibody

Cat. Num. AAA9610309

• Gene Names: USP6; HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: USP6; Polyclonal Antibody; USP6 Antibody; Deubiquitinating enzyme 6; HRP1; Proto-oncogene TRE-2; TRE17; TRE2; Ubiquitin carboxyl-terminal hydrolase 6; Ubiquitin specific protease 6; Ubiquitin thiolesterase 6; Ubiquitin-specific-processing protease 6; UBP6_HUMAN; anti-USP6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: USP6 antibody detects endogenous levels of total USP6
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 1406

• Applicable Applications for anti-USP6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human USP6
• Subcellular Location: Cell Membrane. Cytoplasm. Endosome. Localizes to the plasma membrane and to filamentous structures within the cell corresponding to ARF6 regulated tubular endosomes. Activation of RAC1 and CDC42 can direct the relocalization of USP6 to the plasma membrane in a manner that depends on the integrity of the actin cytoskeleton.
• Tissue Specificity: Testis specific. Expressed in various cancer cell lines.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis USP6 using HeLa whole cell lysates)

Related Product Information for anti-USP6 antibody

Function: Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.
Subunit Structure: Interacts with RAC1 and CDC42. Interacts (via Rab-GAP TBC domain) with ARF6. Interacts with calmodulin (CALM1, CALM2 and/or CALM3); the interaction is calcium-dependent.
Post-translational Modifications: Monubiquitinated; ubiquitination is calmodulin and calcium dependent.
Similarity: The Rab-GAP TBC domain lacks GTPase activator activity but is necessary for interaction with ARF6. Belongs to the peptidase C19 family.

NCBI and Uniprot Product Information

• NCBI GI #: 747165331
• NCBI GeneID: 9098
• NCBI Accession #: NP_001291213.1
• NCBI GenBank Nucleotide #: NM_001304284.1
• UniProt Accession #: P35125; Q15634; Q86WP6; Q8IWT4
• Molecular Weight: Observed: 159 kDa; Predicted: 159 kDa
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 6
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 6
• NCBI Official Symbol: USP6
• NCBI Official Synonym Symbols: HRP1; TRE2; TRE17; Tre-2; TRESMCR; USP6-short
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 6
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 6
• Protein Family: USP6 N-terminal-like protein
• UniProt Gene Name: USP6
• UniProt Synonym Gene Names: HRP1; TRE2

Uniprot Description

Deubiquitinase with an ATP-independent isopeptidase activity, cleaving at the C-terminus of the ubiquitin moiety. Catalyzes its own deubiquitination. In vitro, isoform 2, but not isoform 3, shows deubiquitinating activity. Promotes plasma membrane localization of ARF6 and selectively regulates ARF6-dependent endocytic protein trafficking. Is able to initiate tumorigenesis by inducing the production of matrix metalloproteinases following NF-kappa-B activation.