Rabbit anti-Human RAC1 Polyclonal Antibody | anti-RAC1 antibody

RAC1 Antibody

Cat. Num. AAA7046153

• Gene Names: RAC1; MIG5; Rac-1; TC-25; p21-Rac1
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunofluorescence
• Purity: >95%, Protein G purified
• Synonyms: RAC1; Polyclonal Antibody; RAC1 Antibody; Ras-related C3 botulinum toxin substrate 1; Cell migration-inducing gene 5 protein; Ras-like protein TC25; p21-Rac1; TC25; MIG5; anti-RAC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%, Protein G purified
• Form/Format: Liquid
• Sequence Length: 192

• Applicable Applications for anti-RAC1 antibody: ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Species: Human
• Immunogen: Recombinant human Ras-related C3 botulinum toxin substrate 1 protein
• Conjugate: Non-conjugated
• Buffer: Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-6084 / sc-16698 / sc-34259 / sc-34260 / sc-217 / sc-95
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using MBS7046153 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human spleen tissue using MBS7046153 at dilution of 1:100)

Immunohistochemistry (IHC)

(Immunofluorescent analysis of HeLa cells using MBS7046153 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Related Product Information for anti-RAC1 antibody

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Stimulates PKN2 kinase activity. In concert with RAB7A, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. In glioma cells, promotes cell migration and invasion. In podocytes, promotes nuclear shuttling of NR3C2; this modulation is required for a proper kidney functioning. Required for atypical chemokine receptor ACKR2-induced LIMK1-PAK1-dependent phosphorylation of cofilin (CFL1) and for up-regulation of ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. In synapses, seems to mediate the regulation of F-actin cluster formation performed by SHANK3.

NCBI and Uniprot Product Information

• NCBI GI #: 9845511
• NCBI GeneID: 5879
• NCBI Accession #: NP_008839.2
• NCBI GenBank Nucleotide #: NM_006908.4
• UniProt Accession #: P63000; O95501; P15154; Q3Y4D3; Q5JAA8; Q9BTB4; AAB22206
• Molecular Weight: 23,467 Da
• NCBI Official Full Name: ras-related C3 botulinum toxin substrate 1 isoform Rac1
• NCBI Official Synonym Full Names: ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
• NCBI Official Symbol: RAC1
• NCBI Official Synonym Symbols: MIG5; Rac-1; TC-25; p21-Rac1
• NCBI Protein Information: ras-related C3 botulinum toxin substrate 1
• UniProt Protein Name: Ras-related C3 botulinum toxin substrate 1
• Protein Family: Ras-related protein
• UniProt Gene Name: RAC1
• UniProt Synonym Gene Names: TC25
• UniProt Entry Name: RAC1_HUMAN

NCBI Description

The protein encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Uniprot Description

RAC1: a plasma membrane-associated member of the Rho-GTPase family. Plays a key role in cytoskeletal reorganization, membrane trafficking, transcriptional regulation and cell growth and development. GTP binding stimulates its activity. Phosphorylation by Akt may inhibit GTP binding of Rac1, therefore attenuating the downstream signal transduction pathway. Found in a trimeric complex composed of DOCK1 and ELMO1, which plays a central role in phagocytosis of apoptotic cells. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; G protein, monomeric; G protein, monomeric, Rho; G protein

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: actin filament; cytoplasm; cytosol; endoplasmic reticulum membrane; focal adhesion; lamellipodium; membrane; plasma membrane; trans-Golgi network

Molecular Function: enzyme binding; GTP binding; GTPase activity; protein binding; Rho GDP-dissociation inhibitor binding; thioesterase binding

Biological Process: actin cytoskeleton organization and biogenesis; actin filament polymerization; anatomical structure morphogenesis; blood coagulation; cell adhesion; cell motility; cell motility involved in cell locomotion; ephrin receptor signaling pathway; inflammatory response; lamellipodium biogenesis; localization within membrane; negative regulation of interleukin-23 production; negative regulation of receptor-mediated endocytosis; platelet activation; positive regulation of apoptosis; positive regulation of focal adhesion formation; positive regulation of protein amino acid phosphorylation; positive regulation of Rho protein signal transduction; positive regulation of stress fiber formation; regulation of cell migration; regulation of cell size; regulation of defense response to virus by virus; regulation of hydrogen peroxide metabolic process; regulation of small GTPase mediated signal transduction; response to wounding; ruffle organization and biogenesis; T cell costimulation; vascular endothelial growth factor receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway