Mouse alpha-Gal Epitope Monoclonal Antibody
alpha-Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R)
Applications
ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Supernatant
Synonyms
alpha-Gal Epitope; Monoclonal Antibody; alpha-Gal Epitope (Galalpha1-3Galbeta1-4GlcNAc-R); Anti -alpha-Gal Epitope (Gal 1-3Gal 1-4GlcNAc-R); anti-alpha-Gal Epitope antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgM
Clone Number
6D407
Specificity
Recognizes synthetic and naturally produced rabbit Gal alpha 1-3 Gal epitopes on glycoproteins and glycolipids.
Purity/Purification
Supernatant
Form/Format
Supplied as a liquid.
Applicable Applications for anti-alpha-Gal Epitope antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Functional Studies (can be used for cytotoxicity assays for alpha-Gal specific pathways)
Other applications not tested
Other applications not tested
Application Notes
ELISA: 1:5
Western Blot: 1:5
Flow Cytometry: 1:5
Immunohistochemistry (Paraffin): 1:5
Note: Do not use non-fat milk for blocking. Many blocking agents from non-human mammal sources will crossreact with the antibody.
Optimal dilutions to be determined by the researcher.
Western Blot: 1:5
Flow Cytometry: 1:5
Immunohistochemistry (Paraffin): 1:5
Note: Do not use non-fat milk for blocking. Many blocking agents from non-human mammal sources will crossreact with the antibody.
Optimal dilutions to be determined by the researcher.
Immunogen
Rabbit red blood cell membrane
Cross Reactivity
Mouse, rat and porcine.
Does not crossreact with beta-Gal glycoproteins or BSA.
Does not crossreact with beta-Gal glycoproteins or BSA.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-alpha-Gal Epitope antibody
Widely described antibody against alpha-galactose residues with broad cross-reactivity among different species. Useful for measuring the alpha-Gal epitope expression on cells, glycolipids and glycoproteins, characterization of hyperacute rejection (HAR) in organ and tissue transplantations and monitoring xenotransplantation experiments. The IgM isotype mimics in vivo reactions and can be used for cytotoxicity assays for alpha -Gal specific pathways (addition or presence of complement necessary). Ideal as a high-throughput screening tool for inhibitors of antibody induced cytotoxicity. Organ transplantation from pig to human result in HAR. Humans naturally produce large quantities of anti-alpha-Gal antibodies, which represent 1-3% of all circulating immunoglobulins and are produced by about 1% of all B cells. When pig organs or tissues are transplanted into the human body, the IgM isotype of anti-Gal binds to alpha-Gal epitopes, which causes activation of the complement cascade, resulting in cell lysis. This rapid activation of complement by anti-Gal IgM is an immunological barrier that poses the greatest risk of initiating HAR.
Product Categories/Family for anti-alpha-Gal Epitope antibody
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Product Notes
The alpha-Gal Epitope (Catalog #AAA603355) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's alpha-Gal Epitope can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Functional Studies (can be used for cytotoxicity assays for alpha-Gal specific pathways) Other applications not tested. ELISA: 1:5 Western Blot: 1:5 Flow Cytometry: 1:5 Immunohistochemistry (Paraffin): 1:5 Note: Do not use non-fat milk for blocking. Many blocking agents from non-human mammal sources will crossreact with the antibody. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the alpha-Gal Epitope for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "alpha-Gal Epitope, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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