Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 (MBS488427) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488427, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
LeY recombinant antibody
Anti-LeY [BR96]
Reactivity
Human
Applications
ELISA, Immunohistochemistry
Purity
Protein A Affinity Purified
Synonyms
LeY; Monoclonal Recombinant Antibody; Anti-LeY [BR96]; Lewis Y; LeY recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Mouse IgG1 kappa
Clone Number
BR96
Specificity
The antibody binds Le(Y) with a Ka of 0.1 uM.
Purity/Purification
Protein A Affinity Purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for LeY recombinant antibody
ELISA (EIA), Immunohistochemistry (IHC)
Antibody Type
Recombinant Antibody
Antibody Species
Mouse
Original Species Name
Mouse
Immunogen
H3396 cultured line established from a metastasis of a human breast adenocarcinoma.
Chimeric Use Statement
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.
Clone Note
The antibody binds specifically to LeY tetrasaccharide, a carbohydrate antigen expressed at the cell surface of selective carcinomas, including those of the colon, breast, ovary and lung. Studies have shown that the monoclonal antibody has toxic effects to antigen-positive tumour cells, performing ADCC at concentrations down to 0.1 ug/ml. The antibody B396 was also shown to perform complement-dependent cytotoxicity at concentrations of 0.1-5.0 ug/ml in certain carcinoma cell lines. The monoclonal antibody also has the ability to be internalised by antigen-positive tumour cells, and can thereby be used to deliver anti-cancer drugs to the specific tumour cells.
Buffer Composition
PBS only with 0.02% Proclin 300.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 (MBS488427) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488427, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 (MBS488427) at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488427, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody followed by Alexa Fluor 488 secondary antibody.)
Related Product Information for LeY recombinant antibody
Recombinant monoclonal antibody to LeY. Manufactured using the recombinant platform with variable regions (i.e. specificity) from the hybridoma BR96.
References
Hellström I, Garrigues HJ, Garrigues U, Hellström KE. Highly tumor-reactive, internalizing, mouse monoclonal antibodies to Le(y)-related cell surface antigens. Cancer Res. 1990 Apr 1;50(7):2183-90. PMID:1690595
Original Published Journal: Cancer Res. 1990 Apr 1;50(7):2183-90.
Original Published Journal: Cancer Res. 1990 Apr 1;50(7):2183-90.
Original Published PMID: 7664109
Publication Note: Describes the generation of two monoclonal antibodies, BR64 and BR69, which are selective for cell surface antigens present in certain carcinomas. The antibodies are internalised by antigen-positive tumour cells.
Original Published Journal: Cancer Res. 1990 Apr 1;50(7):2183-90.
Original Published Journal: Cancer Res. 1990 Apr 1;50(7):2183-90.
Original Published PMID: 7664109
Publication Note: Describes the generation of two monoclonal antibodies, BR64 and BR69, which are selective for cell surface antigens present in certain carcinomas. The antibodies are internalised by antigen-positive tumour cells.
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Product Notes
The LeY (Catalog #AAA488427) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-LeY [BR96] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LeY can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the LeY for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LeY, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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