CD64 recombinant antibody
Anti-CD64 [H22]
Gene Names
FCGR1A; CD64; FCRI; CD64A; IGFR1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Protein A Affinity Purified
Synonyms
CD64; Monoclonal Recombinant Antibody; Anti-CD64 [H22]; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA; FcgammaRI; CD64 recombinant antibody
Reactivity
Human
Clonality
Monoclonal
Isotype
Human IgG1, Kappa
Clone Number
H22
Specificity
This immunotoxin is specific for human CD64, and specifically an epitope outside the Fc binding domain. The binding activity of the VH/VL antibody format was unaffected by fusion of the m22(scFv) coding regions to the truncated ETA coding sequences
Purity/Purification
Protein A Affinity Purified
Concentration
1 mg/ml (varies by lot)
Sequence Length
374
Applicable Applications for CD64 recombinant antibody
CM-ELISA (EIA), Flow Cytometry (FC/FACS), Toxicity Assays, Competition Studies
Application Notes
This immunotoxin binds human CD64, and displays specific cytotoxicity against, and thus can be used to selectively eliminate, AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis. Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs.
Immunogen
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A.
Buffer Composition
PBS only with 0.02% Proclin 300.
Chimeric Use Statement
This chimeric human antibody was made using the variable domain sequences of the original IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Labeled Substance
None
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Related Product Information for CD64 recombinant antibody
Recombinant monoclonal antibody to CD64. Manufactured using a proprietary Recombinant Platform with variable regions (i.e. specificity) from the hybridoma H22.
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA).
This recombinant antibody was prepared by cloning the single chain fragment (scFv) derived from the murine anti-human CD64 mAb m22 into the bacterial expression vector pBM1.1, and fusing it to a deletion mutant of Pseudomonas exotoxin A (ETA).
Product Categories/Family for CD64 recombinant antibody
References
Guyre et al., Tur et al. Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function & Recombinant CD64-Specific Single Chain Immunotoxin Exhibits Specific Cytotoxicity against Acute Myeloid Leukemia Cells Journal of immunology, 1 September 1989, Vol.143(5), pp.1650-5 & CANCER RESEARCH 63, 8414-8419, December 1, 2003 PMID:
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,795 Da
NCBI Official Full Name
high affinity immunoglobulin gamma Fc receptor I
NCBI Official Synonym Full Names
Fc fragment of IgG receptor Ia
NCBI Official Symbol
FCGR1A
NCBI Official Synonym Symbols
CD64; FCRI; CD64A; IGFR1
NCBI Protein Information
high affinity immunoglobulin gamma Fc receptor I
UniProt Protein Name
High affinity immunoglobulin gamma Fc receptor I
UniProt Gene Name
FCGR1A
UniProt Synonym Gene Names
FCG1; FCGR1; IGFR1; IgG Fc receptor I; FcRI; FcgammaRIa
NCBI Description
This gene encodes a protein that plays an important role in the immune response. This protein is a high-affinity Fc-gamma receptor. The gene is one of three related gene family members located on chromosome 1. [provided by RefSeq, Jul 2008]
Uniprot Description
High affinity receptor for the Fc region of immunoglobulins gamma. Functions in both innate and adaptive immune responses.
Research Articles on CD64
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Product Notes
The CD64 fcgr1a (Catalog #AAA488343) is a Recombinant Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD64 [H22] reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD64 can be used in a range of immunoassay formats including, but not limited to, CM-ELISA (EIA), Flow Cytometry (FC/FACS), Toxicity Assays, Competition Studies. This immunotoxin binds human CD64, and displays specific cytotoxicity against, and thus can be used to selectively eliminate, AML cells; FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis. Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs. Researchers should empirically determine the suitability of the CD64 fcgr1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD64, Monoclonal Recombinant Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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