Testing Data
CytoSelect LDH Cytotoxicity Assay Kit
CytoSelect LDH Cytotoxicity Assay Kit
Synonyms
CytoSelect LDH Cytotoxicity; CytoSelect LDH Cytotoxicity Assay Kit; CytoSelect LDH Cytotoxicity assay kit
Preparation and Storage
The LDH Cytotoxicity Assay Reagent is a clear, slightly red, ready-to-use solution. Aliquot as needed to avoid repeated freeze-thaw cycles and store at -20 degree C protected from light. If precipitates or turbidity are observed upon thawing, warm the solution to 37 degree C for 5-10 minutes and agitate to dissolve the precipitates. Store the Triton X-100 Solution at Room Temperature.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for CytoSelect LDH Cytotoxicity assay kit
Principle of the assay: CytoSelect LDH Cytotoxicity Assay Kit provides a colorimetric format for measuring and monitoring cell cytotoxicity. The kit contains sufficient reagents for the evaluation of 960 assays in 96-well plates. Cells can be plated and then treated with compounds or agents that affect cell viability. Upon cell death, lactate dehydrogenase (LDH), a soluble enzyme found in the cytoplasm, is released into the growth media. The growth media is then transferred to another plate and the released LDH is then detected with cytotoxicity reagent. In the presence of lactate substrate (included in the LDH Cytotoxicity Reagent) LDH converts lactate to pyruvate and generates nicotinamide adenine dinucleotide (NADH). The WST-1 molecule, also present in the LDH Cytotoxicity Reagent, is converted from WST-1 to the orange formazan form. An increase in cell cytotoxicity is accompanied by increased LDH release and increased colorimetric signal. The assay principles are basic and can be applied to most eukaryotic cell lines, including adherent and non-adherent cells and certain tissues, depending on LDH expression levels. The LDH Cytotoxicity Reagent can be used to detect cytotoxicity in mammalian cells.
Background: The measurement and monitoring of cell cytotoxicity is an essential technique in any laboratory focused on cell-based research. This skill allows for the optimization of cell culture conditions. More importantly, the cytotoxic nature of anticancer compounds in toxicology testing, the toxicity of therapeutic chemicals in drug screening, and cell-mediated cytotoxicity can all be assessed through this assay-based approach. Cell cytotoxicity characteristics include loss of cellular metabolic activity or cell membrane integrity. One method for measuring metabolic activity is to incubate the cells with a tetrazolium salt such as MTT, which is cleaved into a colored formazan product by metabolically active cells. Similarly, the green fluorescent dye Calcein AM can measure intracellular esterase activity in proliferating live cells, while dyes such as trypan blue or propidium iodide can enter and stain cells that have lost membrane integrity.
Product Categories/Family for CytoSelect LDH Cytotoxicity assay kit
References
1. Jacobsen MD, Weil M, Raff MC. (1996) J Cell Biol 133, 1041.
2. Liu X, Cui X, Shan N, Li Y, Fang X, Ding M, Wang X. (2013) Cancer Cell Int. 13, 112
3. Yamaori S, Ishii H, Chiba K, Yamamoto I, Watanabe K (2013) Toxicology 314, 251
4. Wang Y, Qu L, Gong L, Sun L, Gong R, Si J (2013) Cancer Biother Radiopharm. 28, 623
5. Xue TY, He YZ, Zhang JJ, Zhang XY, Xin YJ, Lu Y, Li YH (2013) Clin Lab 59, 613
Chu, J. et al. (2015). Pharmacologic blockade of 12/15-lipoxygenase ameliorates memory deficits, abeta and Tau neuropathology in the triple-transgenic mice. Mol Psychiatry. doi: 10.1038/mp.2014.170.
2. Liu X, Cui X, Shan N, Li Y, Fang X, Ding M, Wang X. (2013) Cancer Cell Int. 13, 112
3. Yamaori S, Ishii H, Chiba K, Yamamoto I, Watanabe K (2013) Toxicology 314, 251
4. Wang Y, Qu L, Gong L, Sun L, Gong R, Si J (2013) Cancer Biother Radiopharm. 28, 623
5. Xue TY, He YZ, Zhang JJ, Zhang XY, Xin YJ, Lu Y, Li YH (2013) Clin Lab 59, 613
Chu, J. et al. (2015). Pharmacologic blockade of 12/15-lipoxygenase ameliorates memory deficits, abeta and Tau neuropathology in the triple-transgenic mice. Mol Psychiatry. doi: 10.1038/mp.2014.170.
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Product Notes
The CytoSelect LDH Cytotoxicity (Catalog #AAA168668) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "CytoSelect LDH Cytotoxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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