Rabbit Macrophages Polyclonal Antibody
Anti-Rat Macrophages, Adsorbed Antiserum, (Polyclonal) (rabbit serum)
Applications
Cytotoxicity Assay, Immunohistochemistry
Synonyms
Macrophages; Polyclonal Antibody; Anti-Rat Macrophages; Adsorbed Antiserum; (Polyclonal) (rabbit serum); Adsorbed Rabbit anti-Rat Macrophage; anti-Macrophages antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
Rabbit Sera
Applicable Applications for anti-Macrophages antibody
Cytotoxic Assay (CA), Immunohistochemistry Frozen/Paraffin (IHC)
Application Notes
This antibody is suitable for use in cytotoxic assays, and immunohistochemistry (frozen and paraffin sections).
Test Type
For Cytotoxic Antibodies:
Modified Colormetric Microtiter Assay 1
Results:
Antisera of this lot(5855AD) diluted 1:80 exhibits >80% cytotoxicity on rat macrophages.
Antisera of this lot(5855AD) diluted 1:80 exhibits <20% cytotoxicity on rat thymocytes.
For Agglutinating Antibodies:
Antisera dilutions in RPMI-1640 incubated with target cells at 4-8°C for 1 hour. Agglutination determined by microscopic observations.
Results: Antisera of this lot (5855AD) strongly agglutinates rat macrophages, but not thymoctyes, at a dilution of 1:128.
Modified Colormetric Microtiter Assay 1
Results:
Antisera of this lot(5855AD) diluted 1:80 exhibits >80% cytotoxicity on rat macrophages.
Antisera of this lot(5855AD) diluted 1:80 exhibits <20% cytotoxicity on rat thymocytes.
For Agglutinating Antibodies:
Antisera dilutions in RPMI-1640 incubated with target cells at 4-8°C for 1 hour. Agglutination determined by microscopic observations.
Results: Antisera of this lot (5855AD) strongly agglutinates rat macrophages, but not thymoctyes, at a dilution of 1:128.
Presentation
2 ml, liquid.
Preservatives
None (0.2 um filtered)
Preparation and Storage
Divide into useful aliquots and store at -20°C.
Related Product Information for anti-Macrophages antibody
MBS524051 is a rabbit antiserum directed against rat macrophages. This antiserum is adsorbed repeatedly with rat red blood cells, thymocytes and spleen cells to remove most antibodies to antigens common among different cell types. This adsorption significantly improves cytotoxic specificity.
Similar Products
Product Notes
The Macrophages (Catalog #AAA524051) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Macrophages can be used in a range of immunoassay formats including, but not limited to, Cytotoxic Assay (CA), Immunohistochemistry Frozen/Paraffin (IHC). This antibody is suitable for use in cytotoxic assays, and immunohistochemistry (frozen and paraffin sections). Researchers should empirically determine the suitability of the Macrophages for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Macrophages, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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