Rabbit anti-Pig LDH Polyclonal Antibody | anti-LDH antibody

LDH antibody

Cat. Num. AAA534030

• Reactivity: Pig
• Applications: User optimized
• Synonyms: LDH; Polyclonal Antibody; LDH antibody; Polyclonal LDH; Anti-LDH; LDH-A; LDH-M; LDH muscle subunit; L-lactate dehydrogenase A chain; anti-LDH antibody

• Host: Rabbit
• Reactivity: Pig
• Clonality: Polyclonal
• Specificity: Pig
• Form/Format: Serum with 0.1% NaN3.

• Applicable Applications for anti-LDH antibody: User optimized
• Sterility: This reagent is not sold as sterile, but can be sterilized by filtration if necessary. To minimize loss of volume during filtration, dilute to the final working concentration in the appropriate medium before filtration and filter through a 0.22 um filter.
• Biological Significance: Lactate dehydrogenase catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. It converts pyruvate, the final product of glycolysis to lactate when oxygen is absent or in short supply, and it performs the reverse reaction during the Cori cycle in the liver. Plasmodium falciparum is a protozoan parasite, one of the species of Plasmodium that cause malaria in humans. It is transmitted by the female Anopheles mosquito.
• Cross-Reactivity: LDH: 100%
• RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF MOUSE LYMPHOCYTES: 1.-Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium1 or equivalent. Remove red cells and dead cells (where necessary) by purification of viable lymphocytes on Lympholyte®-M2 density cell separation medium. After washing, adjust the cell concentration to 1x107 cells per ml in Cytotoxicity Medium.; ; 2.Add the antiserum to a final concentration of 1:40 and mix.; ; 3.Incubate for 60 minutes at 4°C.; ; 4.Centrifuge to pellet the cells and discard the supernatant; ; 5.-Resuspend to the original volume in Low-Tox®-M3 Rabbit Complement, diluted to the appropriate concentration in Cytotoxicity Medium. (Recommended concentration included with each batch of Low-Tox®-M Complement).; ; 6.-Incubate for 60 minutes at 37°C.; ; 7.-Monitor for percent cytotoxicity at this stage, before further processing. For this purpose remove a small sample from each tube, dilute 1:10 with medium, and add 1/10 volume of 1% Trypan Blue. After 3-5 minutes, score live versus dead cells in a hemacytometer. ; ; 8.- For functional studies, remove the dead cells from the treated groups before further processing, particularly if the treated cells are to be cultured. Layering the suspension on cell separation medium and centrifuging at room temperature as per the instructions provided can do this. Live cells will form a layer at the interface, while dead cells pellet. The interface can then be collected and washed in Cytotoxicity Medium before being resuspended in the appropriate medium for further processing.; ; Alternatively, the cells can then be washed and resuspended in the appropriate medium for further processing immediately after Step #6, provided that the dead cells will not interfere with subsequent assays.
• LOTS SPECIFICATIONS: Antiserum Titration: ; ; Cell Source: Thymus; ; Donors :BALB/C; ; Cell concentration: 1.1x10⁶ cells per ml.; ; Complement Low Tox- M RabbitComplement³; ; Complement Concentration: 1:10; ; Procedure: Two Stage cytotoxicity as described. "Recommended Method for Determining Percent Cytotoxicity with Anti-Mouse Lymphocyte Serum Plus Complement ".
• Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
• Immunogen: LDH antibody was raised in rabbit using porcine lactate dehydrogenase-H4 as the immunogen.
• Preparation and Storage: Lyophilized from stable at 4°C or -20°C . Reconstitute with 1.0mL or 5.0 mL distilled water. After reconstitution, aliquot and freeze unused portions at -70°C in volumes appropiate for single usage. Avoid repeated freeze/thaw cycles.

Related Product Information for anti-LDH antibody

Rabbit polyclonal LDH antibody

Product Categories/Family for anti-LDH antibody

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 312132468
• NCBI GeneID: 9958570
• NCBI Accession #: YP_003999807.1
• NCBI GenBank Nucleotide #: NC_014656.1
• NCBI Official Full Name: ldh
• Protein Family: Leucine dehydrogenase