Mouse anti-Mouse NK1.1 (Ly-55) Monoclonal Antibody
Mouse Anti-Mouse NK1.1-APC
Reactivity
Mouse
Applications
Flow Cytometry, Immunoprecipitation
Synonyms
NK1.1 (Ly-55); Monoclonal Antibody; Mouse Anti-Mouse NK1.1-APC; Mouse Anti-Mouse NK1.1 - Allophycocyanin; Mouse Anti-Mouse NK1.1 (Ly-55); anti-NK1.1 (Ly-55) antibody
Host
Mouse
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Mouse [(C3H x BALB/c)F1] IgG2a kappa
Clone Number
PK136
Specificity
Mouse NK1.1 alloantigen, Mr 80-85 kDa (39 kDa under reducing conditions)
NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL, NZB).1-4, 10 NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.5-9
NK1.1, a member of the NKR-P1 family of cell surface receptors, is a type II integral membrane glycoprotein with a C-type lectin domain. It is expressed as a disulfide-linked homodimer on all NK cells, as well as subsets of thymocytes and peripheral T lymphocytes in selected strains of mice (e.g., C57BL, NZB).1-4, 10 NK1.1 mediates cellular activation and differentiation, and is thought to have a particular role in generating Th2 cells. This product does not react with NK cells of BALB/c mice.5-9
Form/Format
Allophycocyanin (APC) Conjugate
Applicable Applications for anti-NK1.1 (Ly-55) antibody
Flow Cytometry; Immunoprecipitation
Application Notes
Research Application(s): Induction of proliferation of a subset of NK cells
In vivo depletion of NK1.1 cells
In vitro redirected lysis
In vitro cytotoxicity assays
Flow Cytometry Suggested Dilution: <=0.5 ug/10^6 cells
In vivo depletion of NK1.1 cells
In vitro redirected lysis
In vitro cytotoxicity assays
Flow Cytometry Suggested Dilution: <=0.5 ug/10^6 cells
Immunogen
CE mouse spleen and bone marrow cells enriched for NK-1^+ cells
Characterization
To ensure lot-to-lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform to characteristics of a standard reference reagent. Representative data are included in this product insert.
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The R-phycoerythrin-Cyanine 7 (R-PE-CY 7 conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The R-phycoerythrin-Cyanine 7 (R-PE-CY 7 conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 degree C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
References
1. Michie. S.A., P.R. Streeter, E.C. Butcher, and R.V. Rouse. 1995. Am. J. Pathol. 147:412.
2. Garni-Wagner, B.A., A. Purohit, P.A. Matthew, M. Bennett, and V. Kumar. 1993. J. Immunol. 151:60.
3. Koo, G.C., F.J. Dumont, M. Tutt, J. Hackett, Jr., and V. Kumar. 1996. J. Immunol. 137:3742.
4. Yokoyama, W.M., and W.E. Seaman. 1993. Annu, Rev. Immunol. 11:613.
5. Sentman, C.L., V. Kumar, G. Koo, and M. Bennett. 1989. J. Immunol. 142:1847.
6. Yu, Y.Y.L., V. Kumar, and M. Bennett. 1992. Annu. Rev. Immuonol. 10:189.
7. Vicari, A.P., and A. Zlotnik. 1996. Immunol. Today 17:71.
8. Lanier, L.L. 1997. Immunity 6:371.
9. Sentman, C.L., J. Hackett, Jr., T.A. Moore, M.M. Tutt, M. Bennett, and V. Kumar. 1989. Hybridoma 8:605.
10. Koo, G., and J. Peppard. 1984. Hybridoma 3:301.
11. Reichlin, A., and W.M. Yokoyama. 1998. Immunol. Cell Biol. 76:143.
12. Karlhofer, F.M., and W.M. Yokoyama. 1991. J. Immunol. 146:3662.
13. Poulton, L.D., M.J. Smyth, C.G. Hawke, P. Silveria, D. Shepherd, O.V. Naidenko, D.I. Godfrey, and A.G. Baxter. 2001. Int. Immunol. 13:887.
14. Routes, J.M., J.C. Ryan, S. Ryan, and M. Nakamura. 2001. Int. Immunol. 13:1301.
2. Garni-Wagner, B.A., A. Purohit, P.A. Matthew, M. Bennett, and V. Kumar. 1993. J. Immunol. 151:60.
3. Koo, G.C., F.J. Dumont, M. Tutt, J. Hackett, Jr., and V. Kumar. 1996. J. Immunol. 137:3742.
4. Yokoyama, W.M., and W.E. Seaman. 1993. Annu, Rev. Immunol. 11:613.
5. Sentman, C.L., V. Kumar, G. Koo, and M. Bennett. 1989. J. Immunol. 142:1847.
6. Yu, Y.Y.L., V. Kumar, and M. Bennett. 1992. Annu. Rev. Immuonol. 10:189.
7. Vicari, A.P., and A. Zlotnik. 1996. Immunol. Today 17:71.
8. Lanier, L.L. 1997. Immunity 6:371.
9. Sentman, C.L., J. Hackett, Jr., T.A. Moore, M.M. Tutt, M. Bennett, and V. Kumar. 1989. Hybridoma 8:605.
10. Koo, G., and J. Peppard. 1984. Hybridoma 3:301.
11. Reichlin, A., and W.M. Yokoyama. 1998. Immunol. Cell Biol. 76:143.
12. Karlhofer, F.M., and W.M. Yokoyama. 1991. J. Immunol. 146:3662.
13. Poulton, L.D., M.J. Smyth, C.G. Hawke, P. Silveria, D. Shepherd, O.V. Naidenko, D.I. Godfrey, and A.G. Baxter. 2001. Int. Immunol. 13:887.
14. Routes, J.M., J.C. Ryan, S. Ryan, and M. Nakamura. 2001. Int. Immunol. 13:1301.
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Product Notes
The NK1.1 (Ly-55) (Catalog #AAA673564) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Mouse NK1.1-APC reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NK1.1 (Ly-55) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry; Immunoprecipitation. Research Application(s): Induction of proliferation of a subset of NK cells In vivo depletion of NK1.1 cells In vitro redirected lysis In vitro cytotoxicity assays Flow Cytometry Suggested Dilution: <=0.5 ug/10^6 cells. Researchers should empirically determine the suitability of the NK1.1 (Ly-55) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NK1.1 (Ly-55), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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