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2786 results for " Autophagy Antibodies" - showing 1-50


Immunofluorescence (IF) (Immunofluorescent analysis of U251 cells, using ATG16L Antibody.U251 cells(right) were treated with Chloroquine (50 uM,16h).MBS9208353 was diluted at 1:25 dilution.Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green).DAPI was used to stain the cell nuclear (blue).)

ATG16L, Polyclonal Antibody (Cat# AAA9208353)

Full Name
ATG16L Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, mouse
Applications
IF, EIA, WB, IHC-P
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from A431 cell line, untreated or treated with chloroquine, 100ng/ml, using Cleaved-APG8a (MAP1LC3A) Antibody (upper) or Beta-actin (lower).)

Cleaved LC3A, Polyclonal Antibody (Cat# AAA9202198)

Full Name
Cleaved LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, mouse (Predicted Reactivity: Rat, Bovine)
Applications
WB, EIA, IHC, IF, ICC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Testing Data TD ( All lanes : Anti-SQSTM1 (p62) Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: THP-1 whole cell lysate Lane 5: PANC-1 whole cell lysate Lane 6: A20 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

SQSTM1 (p62), Polyclonal Antibody (Cat# AAA9204361)

Full Name
SQSTM1 (p62) Antibody (C-term)
Gene Names
SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B; FTDALS3
Reactivity
Human,Mouse,Rat

Predicted: Rat
Applications
WB, IHC-P , IF, E
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)

Rubicon, Polyclonal Antibody (Cat# AAA809904)

Full Name
Rubicon Antibody
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB) (Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using phospho-EphA2 Antibody (pS897) (upper) or Beta-actin (lower).)

Phospho-EphA2 (S897), Polyclonal Antibody (Cat# AAA9214716)

Full Name
Phospho-EphA2 (S897) Antibody
Gene Names
EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6
Reactivity
Human (Predicted Reactivity: Monkey, Mouse)
Applications
WB, DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (Western blot analysis of lysate from SH-SY5Y cell line, using APG5L Antibody (D3). AP1812a was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

ATG5, Polyclonal Antibody (Cat# AAA9206419)

Full Name
ATG5 Antibody (N-term)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human;
(Predicted Reactivity: Zebrafish, Bovine, Mouse, Pig, Rat, Rabbit )
Applications
WB, EIA, IHC-P, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG5 5031 (2 μg/mL), ATG5 64-229 (2 μg/mL), ????-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)

ATG5, Polyclonal Antibody (Cat# AAA151098)

Full Name
ATG5 Antibody
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC
Purity
ATG5 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data(TD) (All lanes : Anti-ATG7 Antibody (C-term) at1:500 dilution Lane 1: Caki-1 whole cell lysateLane 2: Hela whole cell lysate Lane 3: HepG2whole cell lysate Lysates/proteins at 40 µg perlane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 78 kDa Blocking/Dilutionbuffer: 5% NFDM/TBST.)

ATG7, Polyclonal Antibody (Cat# AAA9204169)

Full Name
ATG7 Antibody (C-term)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human, Mouse
Predicted reactivity: Chicken, Rat
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.)

WIPI2, Polyclonal Antibody (Cat# AAA809779)

Full Name
WIPI2 Antibody: ATTO 390
Gene Names
WIPI2; Atg21; ATG18B; CGI-50; WIPI-2
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells, using Ubiquilin1 Antibody (Center). MBS9204638 was diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).)

Ubiquilin1, Polyclonal Antibody (Cat# AAA9204638)

Full Name
Ubiquilin1 Antibody (Center)
Gene Names
UBQLN1; DA41; DSK2; UBQN; XDRP1; PLIC-1
Reactivity
Reactivity: Human, Mouse
Predicted: Rat
Applications
EIA, IHC-P, WB, IF
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (The anti-BECN1 Pab is used in Western blot to detect BECN1 in, from left to right, A2058, Hela, mouse brain, Y79, and HL60 tissue lysates. BECN1(arrow) was detected using the purified Pab.)

Beclin 1, Polyclonal Antibody (Cat# AAA9203173)

Full Name
Beclin 1 Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Pig, Rat)
Applications
FC/FACS, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemistry with Small intestine tissue)

PSEN1, Polyclonal Antibody (Cat# AAA3214122)

Full Name
PSEN1 antibody - middle region
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
Applications
IHC, IP, WB
Purity
Affinity Purified
Pricing
$445/0.1 mL | $1,985/5x0.1 mL
Western Blot (WB) (Western blot analysis of lysate from MCF-7 cell line, using RHBDF2 Antibody (N-term). MBS9210526 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

RHBDF2, Polyclonal Antibody (Cat# AAA9210526)

Full Name
RHBDF2 Antibody (N-term)
Gene Names
RHBDF2; TEC; TOC; TOCG; RHBDL5; RHBDL6; iRhom2
Reactivity
Human
Applications
EIA, IHC, WB
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB) (Western blot analysis of Denatured ATG1 in mouse cerebellum tissue lysates (35ug/lane). ATG1 (arrow) was detected using the purified Pab.)

Denatured ATG1, Polyclonal Antibody (Cat# AAA9201567)

Full Name
Denatured ATG1 Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human, mouse
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa)

Phospho-LC3C (S12), Polyclonal Antibody (Cat# AAA9204824)

Full Name
Phospho-LC3C (S12) Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human
Predicted Reactivity: Zebrafish, Bovine, Mouse, Rat
Applications
WB, DB, EIA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ULK2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

ULK2, Polyclonal Antibody (Cat# AAA9135229)

Full Name
ULK2 Polyclonal Antibody
Gene Names
ULK2; ATG1B; Unc51.2
Reactivity
Human, Rat
Applications
WB
Purity
Affinity Purification
Pricing
$225/0.05 mL | $305/0.1 mL | $465/0.2 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-ATG7 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

ATG7, Polyclonal Antibody (Cat# AAA809427)

Full Name
ATG7 Antibody: Alkaline Phosphatase
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB) (Western blot analysis of anti-ULK3 Antibody (N-term) in mouse heart tissue lysates (35ug/lane).ULK3(arrow) was detected using the purified Pab.)

ULK3, Polyclonal Antibody (Cat# AAA9207520)

Full Name
ULK3 Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~60 in Jurkat.)

SQSTM1, Polyclonal Antibody (Cat# AAA809653)

Full Name
SQSTM1 Antibody: ATTO 390
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human; Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with GABARAP (N-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9204011 GABARAP (N-term) primary antibody (1:500, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). GABARAP immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

GABARAP, Polyclonal Antibody (Cat# AAA9204011)

Full Name
GABARAP Antibody (N-term)
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rabbit, Rat)
Applications
IF, EIA, WB, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Anti-CLN3 Antibody (Center) at 1:1000 dilution + Neuro-2a whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 48 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

CLN3, Polyclonal Antibody (Cat# AAA9211565)

Full Name
CLN3 Antibody (Center)
Gene Names
CLN3; BTS; JNCL
Reactivity
Human, mouse (Predicted Reactivity: Monkey)
Applications
FC/FACS, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of lysates from 293, T47D cell line (from left to right), using APG8c (MAP1LC3C) Antibody ?M1?. MBS9212772 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

LC3, Polyclonal Antibody (Cat# AAA9212772)

Full Name
LC3 Antibody (APG8C) (N-term)
Gene Names
MAP1LC3C; LC3C; ATG8J
Reactivity
Human
Applications
WB, EIA, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (PRKAA2 (Thr172) Antibody western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the PRKAA2 antibody detected the PRKAA2 protein (arrow).)

PRKAA2 (Thr172), Polyclonal Antibody (Cat# AAA9207580)

Full Name
PRKAA2 (Thr172) Antibody (Center)
Gene Names
PRKAA2; AMPK; AMPK2; PRKAA; AMPKa2
Reactivity
Human (Predicted Reactivity: Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (All lanes : Anti-CAT Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Mouse kidney lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

CAT, Polyclonal Antibody (Cat# AAA9205639)

Full Name
CAT Antibody (Center)
Reactivity
Human (Predicted Reactivity: Bovine, Zebrafish, Mouse, Pig, Rat)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)

Rubicon, Polyclonal Antibody (Cat# AAA809917)

Full Name
Rubicon Antibody: HRP
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of mouse brain, using MAP1LC3B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 2min.)

MAP1LC3B, Polyclonal Antibody (Cat# AAA9135571)

Full Name
MAP1LC3B Polyclonal Antibody
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)

Rubicon, Polyclonal Antibody (Cat# AAA809905)

Full Name
Rubicon Antibody: ATTO 390
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-ATG7 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

ATG7, Polyclonal Antibody (Cat# AAA809433)

Full Name
ATG7 Antibody: PerCP
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB) (Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~60 in Jurkat.)

SQSTM1, Polyclonal Antibody (Cat# AAA809661)

Full Name
SQSTM1 Antibody: Alkaline Phosphatase
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human; Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MAP1LC3A antibody.)

MAP1LC3A, Polyclonal Antibody (Cat# AAA9410125)

Full Name
MAP1LC3A Antibody
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL
Western Blot (WB) (hAPG7L-D555 (PEI 1:100)b western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).)

APG7L, Polyclonal Antibody (Cat# AAA9202368)

Full Name
APG7L Antibody (D555)
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human (Predicted Reactivity: Chicken, Mouse, Rat)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-ATG7 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

ATG7, Polyclonal Antibody (Cat# AAA809431)

Full Name
ATG7 Antibody: HRP
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)

IRGM, Polyclonal Antibody (Cat# AAA8000013)

Full Name
IRGM Antibody
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded H. kidney section using ATG3 Antibody. MBS9200107 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

ATG3, Monoclonal Antibody (Cat# AAA9200107)

Full Name
ATG3 Antibody
Gene Names
ATG3; APG3; APG3L; PC3-96; APG3-LIKE
Reactivity
Human, mouse
Applications
EIA, IHC, WB
Purity
Purified Mouse Monoclonal Antibody (Mab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). MBS9203257 was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)

ATG5, Polyclonal Antibody (Cat# AAA9203257)

Full Name
ATG5 Antibody (C-term)
Gene Names
ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)

IRGM, Polyclonal Antibody (Cat# AAA8000029)

Full Name
IRGM Antibody: RPE
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with ATG9A (C-term) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9208371 ATG9A (C-term) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

ATG9A, Polyclonal Antibody (Cat# AAA9208371)

Full Name
ATG9A Antibody (C-term)
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-IRGM Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-IRGM Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Cytoplasmic Vesicle, Phagosome Membrane, Autophagosome Membrane. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) IRGM Antibody. (D) Composite.)

IRGM, Polyclonal Antibody (Cat# AAA8000014)

Full Name
IRGM Antibody: ATTO 390
Gene Names
IRGM; IFI1; IRGM1; LRG47; LRG-47
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB) (APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)

APG10L, Polyclonal Antibody (Cat# AAA9209925)

Full Name
APG10L Antibody (C-term S116)
Gene Names
ATG10; APG10; APG10L; pp12616
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
WB, EIA, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB) (Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 47.7 kDa SQSTM1 protein using Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-SQSTM1 Polyclonal Antibody (SPC-637) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 47.7 kDa. Other Band(s): ~60 in Jurkat.)

SQSTM1, Polyclonal Antibody (Cat# AAA809663)

Full Name
SQSTM1 Antibody: Biotin
Gene Names
SQSTM1; p60; p62; A170; DMRV; OSIL; PDB3; ZIP3; p62B; NADGP; FTDALS3
Reactivity
Human; Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB) (Western blot analysis of extracts of 293T cells, using ATG9A antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

ATG9A, Polyclonal Antibody (Cat# AAA9135595)

Full Name
ATG9A Polyclonal Antibody
Gene Names
ATG9A; mATG9; APG9L1; MGD3208
Reactivity
Human, Mouse, Rat
Applications
WB, IF
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB) (Western blot analysis of Beclin1-BH3 Domain Antibody in mouse stomach tissue lysates (35ug/lane). BECN1 (arrow) was detected using the purified Pab.)

Beclin1-BH3 Domain, Polyclonal Antibody (Cat# AAA9201004)

Full Name
Beclin1-BH3 Domain Antibody
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, mouse (Predicted Reactivity: Bovine, Chicken, Pig, Rat, Xenopus)
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-ATG7 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

ATG7, Polyclonal Antibody (Cat# AAA809420)

Full Name
ATG7 Antibody: ATTO 488
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)

Rubicon, Polyclonal Antibody (Cat# AAA809916)

Full Name
Rubicon Antibody: FITC
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,900/5x0.1 mg
Immunofluorescence (IF) (Fluorescent image of U251 cells stained with MBS9203938 LC3 (APG8A) (P45) antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9203938 LC3 (APG8A) (P45) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.)

LC3, Polyclonal Antibody (Cat# AAA9203938)

Full Name
LC3 Antibody (APG8A) (P45)
Gene Names
MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
EIA, IHC, WB, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using ATG16L1 antibody.)

ATG16L1, Polyclonal Antibody (Cat# AAA9404105)

Full Name
ATG16L1 Antibody
Gene Names
ATG16L1; IBD10; WDR30; APG16L; ATG16A; ATG16L
Reactivity
Human, Mouse, Rat
Applications
WB, IHC, IF, IP
Purity
Antibodies were purified by affinity purification using immunogen.
Pricing
$300/0.05 mL | $390/0.1 mL | $1,610/5x0.1 mL
Immunohistochemistry (IHC) (Immunohistochemistry analysis using Rabbit Anti-ATG7 Polyclonal Antibody . Tissue: Kidney. Species: Human. Fixation: Formalin Fixed Paraffin-Embedded. Primary Antibody: Rabbit Anti-ATG7 Polyclonal Antibody at 1:50 for 30 min at RT. Counterstain: Hematoxylin. Magnification: 10X.)

ATG7, Polyclonal Antibody (Cat# AAA809429)

Full Name
ATG7 Antibody: Biotin
Gene Names
ATG7; GSA7; APG7L; APG7-LIKE
Reactivity
Human; Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Rubicon Polyclonal Antibody . Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Rubicon Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Late Endosome, Lysosome, Early Endosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Rubicon Antibody. (D) Composite.)

Rubicon, Polyclonal Antibody (Cat# AAA809908)

Full Name
Rubicon Antibody: ATTO 594
Gene Names
RUBCN; SCAR15; RUBICON; KIAA0226
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.)

WIPI2, Polyclonal Antibody (Cat# AAA809782)

Full Name
WIPI2 Antibody: ATTO 594
Gene Names
WIPI2; Atg21; ATG18B; CGI-50; WIPI-2
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry (ICC) (Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-WIPI2 Polyclonal Antibody . Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-WIPI2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm . Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) WIPI2 Antibody (D) Composite.)

WIPI2, Polyclonal Antibody (Cat# AAA809793)

Full Name
WIPI2 Antibody: PerCP
Gene Names
WIPI2; Atg21; ATG18B; CGI-50; WIPI-2
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
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