Western Blot (WB)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG5 5031 (2 μg/mL), ATG5 64-229 (2 μg/mL), ????-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)
Chicken ATG5 Polyclonal Antibody | anti-ATG5 antibody
ATG5 Antibody
Western Blot (WB)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG5 5031 (2 μg/mL), ATG5 64-229 (2 μg/mL), ????-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)
Western Blot (WB)
(Figure 2 Independent Antibody Validation (IAV) via Protein Expression Profile in Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG5 5031 (2 μg/mL), ATG5 64-229 (2 μg/mL), ????-actin (1 μg/mL), and GAPDH (0.02 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken or goat anti-mouse IgG HRP conjugate at 1:10000 and 1:5000 dilution, respectively.)
Western Blot (WB)
(Figure 1 Western Blot Validation of ATG5 in Mouse SpleenLoading: 15 μg of lysates per lane.Antibodies: ATG5, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.Lane A: ATG5 5031, 1 μg/mLLane B: ATG5 5031, 2 μg/mL)
Western Blot (WB)
(Figure 1 Western Blot Validation of ATG5 in Mouse SpleenLoading: 15 μg of lysates per lane.Antibodies: ATG5, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.Lane A: ATG5 5031, 1 μg/mLLane B: ATG5 5031, 2 μg/mL)
Western Blot (WB)
(Figure 3 Western Blot Validation of ATG5 in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG 5031 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.)
Western Blot (WB)
(Figure 3 Western Blot Validation of ATG5 in Human Cell LinesLoading: 15 μg of lysates per lane.Antibodies: ATG 5031 (2 μg/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-chicken IgG HRP conjugate at 1:10000 dilution.)
Immunohistochemistry (IHC)
(Figure 3 Immunohistochemistry Validation of ATG5 in Human SpleenImmunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG5 antibody (5031) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-chicken IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
Immunohistochemistry (IHC)
(Figure 3 Immunohistochemistry Validation of ATG5 in Human SpleenImmunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ATG5 antibody (5031) at 2.5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-chicken IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.)
NCBI and Uniprot Product Information
Uniprot Description
ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; Ubiquitin conjugating system; Apoptosis
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme
Molecular Function: protein binding; APG8 conjugating enzyme activity
Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; negative regulation of protein ubiquitination; blood vessel remodeling; negative regulation of interferon type I production; otolith development; autophagic vacuole formation; negative regulation of apoptosis
Research Articles on ATG5
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Product Notes
The ATG5 atg5 (Catalog #AAA151098) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The ATG5 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATG5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). ATG5 antibody can be used for the detection of ATG5 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the ATG5 atg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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