Western Blot (WB)
(hAPG7L-D555 (PEI 1:100)b western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).)
Rabbit APG7L Polyclonal Antibody | anti-ATG7 antibody
APG7L Antibody (D555)
Western Blot (WB)
(hAPG7L-D555 (PEI 1:100)b western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).)
Western Blot (WB)
(hAPG7L-D555 (PEI 1:100)b western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the APG7L antibody detected the APG7L protein (arrow).)
Immunohistochemistry (IHC)
(APG7L Antibody(D555) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(APG7L Antibody(D555) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of APG7L Antibody(D555) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(APG7L Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(APG7L Antibody flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the
protein appears to be required for fusion of peroxisomal and
vacuolar membranes. The protein shows homology to the ATP-binding
and catalytic sites of the E1 ubiquitin activating enzymes.
Zhao, Y., et al. Nat. Cell Biol. 12(7):665-675(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Xue, L.Y., et al. Autophagy 6(2):248-255(2010)
Zhu, K., et al. Oncogene 29(3):451-462(2010)
NCBI and Uniprot Product Information
NCBI Description
This gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In the yeast, the protein appears to be required for fusion of peroxisomal and vacuolar membranes. The protein shows homology to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes. [provided by RefSeq, Jan 2009]
Uniprot Description
ATG7: Functions as an E1 enzyme essential for multisubstrates such as ATG8-like proteins and ATG12. Forms intermediate conjugates with ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). PE-conjugation to ATG8-like proteins is essential for autophagy. Also acts as an E1 enzyme for ATG12 conjugation to ATG5 and ATG3. Homodimer. Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5. Widely expressed, especially in kidney, liver, lymph nodes and bone marrow. Belongs to the ATG7 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Ubiquitin conjugating system; Autophagy
Chromosomal Location of Human Ortholog: 3p25.3
Cellular Component: cytoplasm; axoneme; cytosol
Molecular Function: protein binding; protein homodimerization activity; APG8 activating enzyme activity; APG12 activating enzyme activity; ubiquitin activating enzyme activity; transcription factor binding
Biological Process: positive regulation of apoptosis; regulation of protein ubiquitination; protein ubiquitination; pyramidal neuron development; adult walking behavior; post-embryonic development; protein transport; cardiac muscle cell development; protein amino acid lipidation; protein catabolic process; positive regulation of autophagy; amino acid metabolic process; central nervous system neuron axonogenesis; cerebellar Purkinje cell layer development; protein modification process; C-terminal protein lipidation; cellular response to starvation; liver development; cellular response to nitrogen starvation; neurological system process; protein modification by small protein conjugation; positive regulation of protein catabolic process; mitochondrion degradation; positive regulation of protein modification process; cerebral cortex development; positive regulation of macroautophagy; negative regulation of apoptosis
Research Articles on ATG7
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Product Notes
The ATG7 atg7 (Catalog #AAA9202368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 540-569. The APG7L Antibody (D555) reacts with Human (Predicted Reactivity: Chicken, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's APG7L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the ATG7 atg7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APG7L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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