Western Blot (WB)
(Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa)
Rabbit Phospho-LC3C (S12) Polyclonal Antibody | anti-MAP1LC3A antibody
Phospho-LC3C (S12) Antibody
Predicted Reactivity: Zebrafish, Bovine, Mouse, Rat
Predicted Reactivity: Zebrafish, Bovine, Mouse, Rat
DB~~1:500
Western Blot (WB)
(Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa)
Western Blot (WB)
(Immunoblots of phosphorylated LC3 (phospho-LC3) in CHO cell culture. LC3 and LC3 S12A mutant vectors were transfected into CHO cells. The cell lysates were separated with SDS-PAGE and blotted with anti-phospho-LC3 S12 antibody. LC3 = microtubule-associated protein light chain-3; S12A = replacement of the amino acid position 12 serine of LC3 with alanine. WT = wildtype LC3-transfected cell lysates; S12A = LC3 S12A mutant-transfected cell lysates; Empty vector = vector with no LC3 gene. Molecular size: LC3-I = 16kDa, and LC3-II = 14 kDa)
Dot Blot (DB)
(Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Dot Blot (DB)
(Dot blot analysis of Phospho-LC3 (APG8a) - S12 Antibody and Nonphospho-LC3 (APG8a) Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Western Blot (WB)
(Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with MBS9204824. The data shows that treatment with rapamycin showed no significant change in level of LC3.)
Western Blot (WB)
(Immunoblots of SH-SY5Y cells treated with rapamycin for 1 h was probed with MBS9204824. The data shows that treatment with rapamycin showed no significant change in level of LC3.)
Western Blot (WB)
(Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with MBS9204824. The data shows that treatment with MPP+ showed no significant change in level of LC3.)
Western Blot (WB)
(Immunoblots of SH-SY5Y cells treated with MPP+ for 24h was probed with MBS9204824. The data shows that treatment with MPP+ showed no significant change in level of LC3.)
Western Blot (WB)
(Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.)
Western Blot (WB)
(Something like SH-SY5Y cells expressing GFP-LC3-WT or-S12D treated with rapamycin or vehicle for 1h.)
NCBI and Uniprot Product Information
NCBI Description
MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012]
Uniprot Description
LC3A: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Two isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; Ubiquitin-like modifier; Microtubule-binding; Vesicle
Chromosomal Location of Human Ortholog: 20q11.22
Cellular Component: microtubule; extrinsic to membrane; late endosome; autophagic vacuole; cytoplasmic vesicle; cytosol
Molecular Function: protein binding; phosphatidylethanolamine binding; microtubule binding; GABA receptor binding; phospholipid binding
Biological Process: mitochondrion degradation; cellular response to nitrogen starvation; autophagic vacuole formation
Research Articles on MAP1LC3A
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Product Notes
The MAP1LC3A map1lc3a (Catalog #AAA9204824) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-LC3C (S12) Antibody reacts with Human Predicted Reactivity: Zebrafish, Bovine, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Phospho-LC3C (S12) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Dot Blot (DB), ELISA (EIA). WB~~1:1000 DB~~1:500. Researchers should empirically determine the suitability of the MAP1LC3A map1lc3a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phospho-LC3C (S12), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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