Western Blot (WB)
(APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)
Rabbit APG10L Polyclonal Antibody | anti-ATG10 antibody
APG10L Antibody (C-term S116)
Western Blot (WB)
(APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)
Western Blot (WB)
(APG10L Antibody (C-term S116) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the APG10L antibody detected the APG10L protein (arrow).)
Western Blot (WB)
(Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.)
Western Blot (WB)
(Western blot analysis of APG10L (arrow) using rabbit polyclonal APG10L Antibody (C-term S116). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG10L gene.)
Immunohistochemistry (IHC)
(APG10L Antibody (C-term S116) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(APG10L Antibody (C-term S116) immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of ANKS1B APG10L Antibody (C-term S116) for immunohistochemistry. Clinical relevance has not been evaluated.)
cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme
involved in 2 ubiquitin-like modifications essential for
autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261)
conjugation and modification of a soluble form of MAP-LC3
(MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a
membrane-bound form (Nemoto et al., 2003 [PubMed
12890687]).
Criollo, A., et al. Cell Death Differ. 14(5):1029-1039(2007)
Shao, Y., et al. Autophagy 3(1):10-16(2007)
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Boya, P., et al. Mol. Cell. Biol. 25(3):1025-1040(2005)
NCBI and Uniprot Product Information
NCBI Description
Autophagy is a process for the bulk degradation of cytosolic compartments by lysosomes. ATG10 is an E2-like enzyme involved in 2 ubiquitin-like modifications essential for autophagosome formation: ATG12 (MIM 609608)-ATG5 (MIM 604261) conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A; MIM 601242), a homolog of yeast Apg8, to a membrane-bound form (Nemoto et al., 2003 [PubMed 12890687]).[supplied by OMIM, Mar 2008]
Uniprot Description
ATG10: E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3. Belongs to the ATG10 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.3.2.-; Autophagy; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q14.1
Cellular Component: cytoplasm; intracellular
Molecular Function: APG12 conjugating enzyme activity; ligase activity
Biological Process: protein transport; protein modification by small protein conjugation; positive regulation of protein modification process; protein amino acid lipidation; autophagy
Research Articles on ATG10
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Product Notes
The ATG10 atg10 (Catalog #AAA9209925) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 96-121. The APG10L Antibody (C-term S116) reacts with Human (Predicted Reactivity: Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's APG10L can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the ATG10 atg10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "APG10L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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