Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). MBS9203257 was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
Rabbit anti-Human, mouse ATG5 Polyclonal Antibody | anti-ATG5 antibody
ATG5 Antibody (C-term)
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). MBS9203257 was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Fluorescent image of U251 cells stained with ATG5 Antibody (C-term). MBS9203257 was diluted at 1:25 dilution. U251 cells were treated with Chloroquine (50 uM, 16h), An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue).)
Western Blot (WB)
(Western blot analysis of APG5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates.)
Western Blot (WB)
(Western blot analysis of APG5L Pab in Y79 cell line, mouse liver tissue, and Hela cell line lysates.)
Western Blot (WB)
(Cos7, HEK293, MEF, and Hela cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.)
Western Blot (WB)
(Cos7, HEK293, MEF, and Hela cells left to right, respectively. Data courtesy of Drs. Jiefei Geng and Dan Klionsky, University of Michigan.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.)
Western Blot (WB)
(Western blot analysis of APG5L (arrow) using rabbit polyclonal APG5L Antibody (P224). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APG5L gene.)
NCBI and Uniprot Product Information
Uniprot Description
ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Autophagy; Apoptosis; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 6q21
Cellular Component: membrane; cytoplasm; pre-autophagosomal structure membrane; autophagic vacuole; axoneme
Molecular Function: protein binding; APG8 conjugating enzyme activity
Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; autophagy; innate immune response; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation
Research Articles on ATG5
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Product Notes
The ATG5 atg5 (Catalog #AAA9203257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 209-238. The ATG5 Antibody (C-term) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ATG5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF). IF~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the ATG5 atg5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATG5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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