TLR2 recombinant protein

TLR2 Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123463

• Purity: >90% as determined by SDS-PAGE
• Synonyms: TLR2; TLR2 Protein; Rat; Recombinant (His Tag); Rat TLR2 Protein (His Tag); toll-like receptor 2; TLR2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH7.4, 10% gly. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gln587

• Species: Rat
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 17
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mature form of rat TLR2 (Q6YGU2)(Met1-Gln587) was expressed,with a polyhistide tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for TLR2 recombinant protein

Background: TLR2, also known as CD282, is a member of the Toll-like receptor (TLR) family. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They play a fundamental role in pathogen recognition and activation of innate immunity. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. TLR2 contains 14 LRR (leucine-rich) repeats and 1 TIR domain. TLR2 gene is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. CD282 cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. It also cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. CD282 acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. It may also promote apoptosis in response to lipoproteins.

Product Categories/Family for TLR2 recombinant protein

Neuroinflammation

References

Do KN, et al. (2012) TLR2 controls intestinal carcinogen detoxication by CYP1A1. PLoS ONE. 7 (3): e32309.Dziarski R, et al. (2001) Role of MD-2 in TLR2- and TLR4-mediated recognition of Gram-negative and Gram-positive bacteria and activation of chemokine genes. J Endotoxin Res. 6 (5): 401-5.Lorenz E, (2007) TLR2 and TLR4 expression during bacterial infections. Curr Pharm Des. 12 (32): 4185-93.

NCBI and Uniprot Product Information

• NCBI GI #: 38454274
• NCBI GeneID: 310553
• NCBI Accession #: NP_942064.1
• NCBI GenBank Nucleotide #: NM_198769.2
• UniProt Accession #: Q6YGU2
• Molecular Weight: 89,413 Da
• NCBI Official Full Name: toll-like receptor 2
• NCBI Official Synonym Full Names: toll-like receptor 2
• NCBI Official Symbol: Tlr2
• NCBI Protein Information: toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2
• UniProt Protein Name: Toll-like receptor
• Protein Family: Toll-like receptor
• UniProt Gene Name: Tlr2
• UniProt Entry Name: Q6YGU2_RAT

NCBI Description

receptor for whom the bell...; involved in the response to bacteria and injury [RGD]

Uniprot Description

TLR2: Cooperates with LY96 to mediate the innate immune response to bacterial lipoproteins and other microbial cell wall components. Cooperates with TLR1 to mediate the innate immune response to bacterial lipoproteins or lipopeptides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. May also promote apoptosis in response to lipoproteins. Recognizes mycoplasmal macrophage- activating lipopeptide-2kD (MALP-2), soluble tuberculosis factor (STF), phenol-soluble modulin (PSM) and B.burgdorferi outer surface protein A lipoprotein (OspA-L) cooperatively with TLR6. Interacts with LY96, TLR1 and TLR6 (via extracellular domain). Binds MYD88 (via TIR domain). Interacts with TICAM1. Ligand binding induces the formation of a heterodimer with TLR1. Interacts with CNPY3. Highly expressed in peripheral blood leukocytes, in particular in monocytes, in bone marrow, lymph node and in spleen. Also detected in lung and in fetal liver. Levels are low in other tissues. Belongs to the Toll-like receptor family.

Protein type: Apoptosis; Motility/polarity/chemotaxis; Receptor, misc.; Cell surface; Membrane protein, integral

Cellular Component: cell surface; cell projection; cytoplasm; plasma membrane; integral to membrane; external side of plasma membrane

Molecular Function: protein binding; peptidoglycan binding; transmembrane receptor activity; protein heterodimerization activity; triacylated lipoprotein binding; receptor activity; pattern recognition receptor activity; diacylated lipoprotein binding

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of interleukin-12 production; response to toxin; positive regulation of leukocyte migration; microglial cell activation; leukotriene metabolic process; response to lipopolysaccharide; positive regulation of NF-kappaB import into nucleus; response to molecule of fungal origin; detection of triacylated bacterial lipoprotein; positive regulation of interleukin-18 production; nitric oxide metabolic process; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; response to insulin stimulus; positive regulation of interleukin-8 production; negative regulation of cell proliferation; response to peptidoglycan; positive regulation of interferon-beta production; response to bacterial lipoprotein; positive regulation of oligodendrocyte differentiation; inflammatory response; positive regulation of Wnt receptor signaling pathway; positive regulation of tumor necrosis factor biosynthetic process; negative regulation of interleukin-12 production; detection of diacylated bacterial lipoprotein; cell surface pattern recognition receptor signaling pathway; positive regulation of interleukin-6 production; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of toll-like receptor signaling pathway; toll-like receptor 2 signaling pathway; induction by symbiont of defense-related host nitric oxide production; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; negative regulation of interleukin-17 production; myelin formation in the central nervous system; response to bacterium; response to hypoxia; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; immune response; I-kappaB phosphorylation; response to progesterone stimulus; chloramphenicol transport; positive regulation of nitric-oxide synthase biosynthetic process; positive regulation of inflammatory response; positive regulation of cytokine secretion