MSRB3 recombinant protein

MSRB3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121819

• Gene Names: MSRB3; DFNB74
• Purity: >95% as determined by SDS-PAGE
• Synonyms: MSRB3; MSRB3 Protein; Human; Recombinant (His Tag); Human MSRB3 Protein (His Tag); DFNB74 Protein; methionine sulfoxide reductase B3; MSRB3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly26-Leu185

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human MSRB3(Q8IXL7-2)(Gly26-Leu185) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MSRB3 recombinant protein

Background: MSRB3 (Methionine Sulfoxide Reductase B3) is a Protein Coding gene. The protein encoded by this gene catalyzes the reduction of methionine sulfoxide to methionine. This enzyme acts as a monomer and requires zinc as a cofactor. This cysteine residue of MSRB3 is conserved in orthologs from yeast to humans and is involved in binding structural zinc. The affected individuals of six unrelated families were homozygous for the same transversion (c.265T>G) in MSRB3. In the inner ear, it is found in the sensory epithelium of the organ of Corti and vestibular end organs as well as in cells of the spiral ganglion. MSRB3-catalyzed reduction of methionine sulfoxides to methionine is essential for hearing. Diseases associated with MSRB3 include Deafness, Autosomal Recessive 74, and Autosomal Recessive Non-Syndromic Sensorineural Deafness Type Dfnb.

NCBI and Uniprot Product Information

• NCBI GI #: 73089054
• NCBI GeneID: 253827
• NCBI Accession #: NP_001026849.1
• NCBI GenBank Nucleotide #: NM_001031679.2
• UniProt Accession #: Q8IXL7; Q6UXS2; B4DR19; B7ZAQ0
• Molecular Weight: 20,010 Da
• NCBI Official Full Name: methionine-R-sulfoxide reductase B3 isoform 2
• NCBI Official Synonym Full Names: methionine sulfoxide reductase B3
• NCBI Official Symbol: MSRB3
• NCBI Official Synonym Symbols: DFNB74
• NCBI Protein Information: methionine-R-sulfoxide reductase B3; methionine-R-sulfoxide reductase B3, mitochondrial
• UniProt Protein Name: Methionine-R-sulfoxide reductase B3
• Protein Family: Methionine-R-sulfoxide reductase
• UniProt Gene Name: MSRB3
• UniProt Synonym Gene Names: MsrB3
• UniProt Entry Name: MSRB3_HUMAN

NCBI Description

The protein encoded by this gene catalyzes the reduction of methionine sulfoxide to methionine. This enzyme acts as a monomer and requires zinc as a cofactor. Several transcript variants encoding two different isoforms have been found for this gene. One of the isoforms localizes to mitochondria while the other localizes to endoplasmic reticula. [provided by RefSeq, Jul 2010]

Uniprot Description

MSRB3: Catalyzes the reduction of free and protein-bound methionine sulfoxide to methionine. Isoform 2 is essential for hearing. Defects in MSRB3 are the cause of deafness autosomal recessive type 74 (DFNB74). A form of non-syndromic sensorineural deafness characterized by prelingual, bilateral, profound hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. A nonsense mutation affecting exclusively mitochondrial isoform 2 is sufficient to produce hearing loss. Belongs to the MsrB Met sulfoxide reductase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; EC 1.8.4.-; Oxidoreductase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: mitochondrion; endoplasmic reticulum

Molecular Function: zinc ion binding; peptide-methionine (R)-S-oxide reductase activity

Biological Process: protein repair; response to oxidative stress

Disease: Deafness, Autosomal Recessive 74