SDPR recombinant protein

Recombinant Mouse SDPR Protein (aa 2-180, His tag)

Cat. Num. AAA2546889

• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: SDPR; Recombinant Mouse SDPR Protein (aa 2-180; His tag); SDPR recombinant protein

• Host: E Coli
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 30% glycerol
• Sequence Length: 418

• Application Notes: The recombinant mouse SDPR consisting of 186 amino acids and has a calculated molecular mass of 21 kDa. rmSDPR migrates as an approximately 28 kDa band in SDS-PAGE under reducing conditions
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SDPR recombinant protein

Background: Mouse Serum deprivation-response protein, also known as Phosphatidylserine-binding protein, Cavin-2 and SDPR, is a member of the PTRF / SDPR family. SDPR is highly expressed in heart and lung, and expressed at lower levels in brain, kidney, liver, pancreas, placenta, and skeletal muscle. SDPR is a new regulator of caveolae biogenesis. SDPR is up-regulated in asyncronously growing fibroblasts following serum deprivation but not following contact inhibition and Down-regulated during synchronous cell cycle re-entry. Caveolae are plasma membrane invaginations with a characteristic flask-shaped morphology. They function in diverse cellular processes, including endocytosis. Loss of SDPR causes loss of caveolae. SDPR binds directly to PTRF and recruits PTRF to caveolar membranes. Overexpression of SDPR, unlike PTRF, induces deformation of caveolae and extensive tubulation of the plasma membrane. SDPR overexpression results in increased caveolae size and leads to the formation of caveolae-derived tubules containing Shiga toxin. SDPR is a membrane curvature inducing component of caveolae, and that STB-induced membrane tubulation is facilitated by caveolae. Pleckstrin and SDPR are phosphorylated by protein kinase C (PKC), the interaction between pleckstrin and SDPR was shown to be independent of PKC inhibition or activation. SDPR may facilitate the translocation of nonphosphorylated pleckstrin to the plasma membrane in conjunction with phosphoinositides that bind to the C-terminal PH domain.

Description: A DNA sequence encoding the mouse SDPR (NP_620080.1) N-terminal segment (Gly 2-Ala 180) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 20270267
• NCBI GeneID: 20324
• NCBI Accession #: NP_620080.1
• NCBI GenBank Nucleotide #: NM_138741.1
• UniProt Accession #: Q63918; Q3V1P6; Q78EC3; Q8CBT4
• Molecular Weight: 46,764 Da
• NCBI Official Full Name: serum deprivation-response protein
• NCBI Official Synonym Full Names: serum deprivation response
• NCBI Official Symbol: Sdpr
• NCBI Protein Information: serum deprivation-response protein
• UniProt Protein Name: Serum deprivation-response protein
• Protein Family: Serum deprivation-response protein
• UniProt Gene Name: Sdpr

Uniprot Description

SDPR: May play a role in targeting PRKCA to caveolae. Belongs to the PTRF/SDPR family.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 1|1 C1.1

Cellular Component: caveola; cytoplasm; cytosol; lipid raft

Molecular Function: phosphatidylserine binding; protein kinase C binding

Biological Process: positive regulation of transcription from RNA polymerase II promoter