S100A14 recombinant protein

S100A14 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121134

• Gene Names: S100A14; BCMP84; S100A15
• Purity: >85% as determined by SDS-PAGE
• Synonyms: S100A14; S100A14 Protein; Human; Recombinant (His Tag); Human S100A14/S114 Protein (His Tag); BCMP84 Protein; S100A15 Protein; S100 calcium binding protein A14; S100A14 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 20% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly2-His104

• Species: Human
• Predicted N Terminal: Met
• Tag: N-His
• Protein Construction: A DNA sequence encoding the native human S100A14 (NP_065723.1)(Gly 2-His 104) was expressed, with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for S100A14 recombinant protein

Background: S100 protein is a family of low molecular weight protein found in vertebrates characterized by two EF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is 100% soluble in ammonium sulfate at neutral pH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from theneural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A14, also known as S100 calciumbinding protein A14, S114 and S100A14, is a cytoplasm protein which belongs to the S-100 family. It is expressed at highest levels in colon and at moderate levels in thymus, kidney, liver, small intestine, and lung. Low expression in heart and no expression is seen in brain, skeletal muscle, spleen, placenta and peripheral blood leukocytes.

References

Pietas A., et al., 2002, Genomics 79: 513-22. Donato, R. et al., 2003, Microsc. Res. Tech. 60 (6): 540-51. Gebhardt, C. et al., 2006, Biochem Pharmacol. 72 (11):1622-31. Nonaka, D. et al., 2008, J. Cutan. Pathol. 35 (11): 1014-9. Lim, SY. et al., 2008, J Immunol. 181 (8): 5627-36. Heibeck T.H. et al., 2009, J. Proteome Res. 8:3852-61.

NCBI and Uniprot Product Information

• NCBI GI #: 10190712
• NCBI GeneID: 57402
• NCBI Accession #: NP_065723.1
• NCBI GenBank Nucleotide #: NM_020672.2
• UniProt Accession #: Q9HCY8; Q5RHT0
• Molecular Weight: 11,662 Da
• NCBI Official Full Name: protein S100-A14
• NCBI Official Synonym Full Names: S100 calcium binding protein A14
• NCBI Official Symbol: S100A14
• NCBI Official Synonym Symbols: BCMP84; S100A15
• NCBI Protein Information: protein S100-A14; S100 calcium-binding protein A14; S114; breast cancer membrane protein 84
• UniProt Protein Name: Protein S100-A14
• Protein Family: Protein
• UniProt Gene Name: S100A14
• UniProt Synonym Gene Names: S100A15; S114
• UniProt Entry Name: S10AE_HUMAN

NCBI Description

This gene encodes a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011]

Uniprot Description

S100A14: a member of the S100 protein family which contains an EF-hand motif and binds calcium. The gene is located in a cluster of S100 genes on chromosome 1. Levels of the encoded protein have been found to be lower in cancerous tissue and associated with metastasis suggesting a tumor suppressor function (PMID: 19956863, 19351828). [provided by RefSeq, Dec 2011]

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: microtubule cytoskeleton; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; plasma membrane; cell junction

Molecular Function: chemokine receptor binding; calcium ion binding

Biological Process: calcium ion homeostasis; apoptosis; defense response to bacterium; response to lipopolysaccharide; toll-like receptor 4 signaling pathway